A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.

Annotation

technique for exploring an unknown region of a CHROMOSOME

DUI

D016386 MeSH Browser

CUI

M0025064

Previous indexing

Chromosome Mapping (1983-1990); Cloning, Molecular (1983-1990)

History note

91

Public note

91