Heteroduplex Analysis [heteroduplexní analýza]

topical
7
Persistent link   https://www.medvik.cz/link/D020180
Definition

A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.

DUI
D020180 MeSH Browser
CUI
M0029895
Previous indexing
Nucleic Acid Heteroduplexes (1980-1998); Nucleic Acid Hybridization (1973-1979)
History note
1999
Public note
1999

Allowable subheadings

CL
classification 0
EC
economics 0
ES
ethics 0
HI
history 0
IS
instrumentation 0
MT
methods 4
ST
standards 0
SN
statistics & numerical data 0
TD
trends 0
VE
veterinary 0

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E01 Diagnosis 1 790
E02 Therapeutics 2 575
E03 Anesthesia and Analgesia 168
E04 Surgical Procedures, Operative 4 387
E05 Investigative Techniques 209
E05.393 Genetic Techniques 579
E05.393.661 Nucleic Acid Hybridization 171
E05.393.661.124 Branched DNA Signal Amplification Assay 1
E05.393.661.187 Comparative Genomic Hybridization 118
E05.393.661.250 Heteroduplex Analysis 7
E05.393.661.475 In Situ Hybridization 159
E05.393.661.640 Oligonucleotide Array Sequence Analysis 256
E05.393.661.820 Subtractive Hybridization Techniques 1
E06 Dentistry 1 666
E07 Equipment and Supplies 4 035

Add