heteroduplexní analýza [Heteroduplex Analysis]

tematický
7
Perzistentní odkaz   https://www.medvik.cz/link/D020180
Definice

A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.

DUI
D020180 MeSH Prohlížeč
CUI
M0029895
Předchozí užití
Nucleic Acid Heteroduplexes (1980-1998); Nucleic Acid Hybridization (1973-1979)
Historická pozn.
1999
Veřejná pozn.
1999