heteroduplexní analýza [Heteroduplex Analysis]
A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions.
- DUI
- D020180 MeSH Prohlížeč
- CUI
- M0029895
- Předchozí užití
- Nucleic Acid Heteroduplexes (1980-1998); Nucleic Acid Hybridization (1973-1979)
- Historická pozn.
- 1999
- Veřejná pozn.
- 1999
Povolená podhesla
- HI
- dějiny 0
- EC
- ekonomika 0
- ES
- etika 0
- CL
- klasifikace 0
- MT
- metody 4
- ST
- normy 0
- IS
- přístrojové vybavení 0
- SN
- statistika a číselné údaje 0
- TD
- trendy 0
- VE
- veterinární 0