Comet Assay [kometový test]

topical
96
Terms

alkalická elektroforéza genomu jedné buňky
alkalický kometový test
gelová elektroforéza genomu jedné buňky

 

Alkaline Comet Assay
Alkaline Single-Cell Gel Electrophoresis Assay
Electrophoresis, Gel, Single-Cell
Gel Electrophoresis, Single-Cell

Persistent link   https://www.medvik.cz/link/D020552
Definition

A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a comet with tail formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.

DUI
D020552 MeSH Browser
CUI
M0328445
Previous indexing
Electrophoresis (1990-1999); Electrophoresis, Agar Gel (1990-1999); Mutagenicity Tests (1992-1999)
History note
2000
Public note
2000

Allowable subheadings

CL
classification
EC
economics 1
ES
ethics
HI
history
IS
instrumentation 1
MT
methods 26
ST
standards 1
SN
statistics & numerical data 3
TD
trends 3
VE
veterinary 1

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E05 Investigative Techniques 209
E05.196 Chemistry Techniques, Analytical 1 182
E05.196.401 Electrophoresis 878
E05.196.401.153 Electrophoresis, Agar Gel 100
E05.196.401.153.150 Comet Assay 96
E05.301 Electrochemical Techniques 208
E05.301.300 Electrophoresis 878
E05.301.300.100 Electrophoresis, Agar Gel 100
E05.301.300.100.150 Comet Assay 96
E05.393 Genetic Techniques 579
E05.393.560 Mutagenicity Tests 441
E05.393.560.150 Comet Assay 96
E05.393.560.598 Micronucleus Tests 68
E05.940 Toxicity Tests 408
E05.940.560 Mutagenicity Tests 441
E05.940.560.150 Comet Assay 96

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