An arsenic (ars) four-gene operon, containing genes encoding a putative membrane permease (ArsP), a transcriptional repressor (ArsR), an arsenate reductase (ArsC) and an arsenical-resistance membrane transporter (Acr3) was first identified in urease-positive thermophilic Campylobacter (UPTC) isolate, CF89-12. UPTC CF89-12 and some other Campylobacter lari isolates contained their ars four-genes, similarly, differing from that in the reference C. lari RM2100 strain. Two putative promoters and a putative terminator were identified for the operon in UPTC CF89-12. In vivo transcription of the operon was confirmed in the UPTC cells. PCR experiments using two primer pairs designed in silico to amplify two arsR and arsC-acr3 segments, respectively, generated two amplicons, approximately 200 and 350 base pairs, with all 31 of 31 and 19 of 31 C. lari isolates (n = 17 for UPTC; n = 14 for UN C. lari), respectively. An inverted repeat forming a dyad structure, a potential binding site for a transcriptional repressor, was identified in the promoter region. Within the deduced 61 amino acids sequence of the putative arsR open reading frame from the UPTC CF89-12, a metal binding box and a DNA-binding helix-turn-helix motif were identified. The UPTC CF89-12 and some other UPTC isolates isolated from natural environment were resistant to arsenate.
- MeSH
- arsen * MeSH
- arsenát reduktasy genetika MeSH
- bakteriální geny * MeSH
- bakteriální RNA genetika MeSH
- Campylobacter lari genetika izolace a purifikace MeSH
- DNA bakterií genetika MeSH
- DNA primery MeSH
- genetické lokusy MeSH
- konformace nukleové kyseliny MeSH
- molekulární sekvence - údaje MeSH
- motiv helix-turn-helix genetika MeSH
- operon genetika MeSH
- otevřené čtecí rámce MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza DNA MeSH
- sekvenční seřazení MeSH
- ureasa genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
