- MeSH
- experimentální nádory genetika MeSH
- geneticky modifikovaná zvířata MeSH
- geny env MeSH
- geny src MeSH
- hlavní histokompatibilní komplex MeSH
- inbrední kmeny zvířat MeSH
- kur domácí * MeSH
- laboratorní zvířata MeSH
- metastázy nádorů MeSH
- modely nemocí na zvířatech * MeSH
- onkogeny MeSH
- ptačí sarkom * MeSH
- rejekce štěpu MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- dějiny 20. století MeSH
- dějiny 21. století MeSH
- geny src * MeSH
- heterografty MeSH
- integrace viru MeSH
- krysa rodu rattus MeSH
- kur domácí virologie MeSH
- nádorové buněčné linie MeSH
- nemoci drůbeže dějiny virologie MeSH
- periodika jako téma dějiny MeSH
- proviry genetika fyziologie MeSH
- ptačí sarkom dějiny virologie MeSH
- transplantace nádorů MeSH
- virová transformace buněk MeSH
- virus Rousova sarkomu genetika izolace a purifikace fyziologie MeSH
- zvířata MeSH
- Check Tag
- dějiny 20. století MeSH
- dějiny 21. století MeSH
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- autobiografie MeSH
- biografie MeSH
- historické články MeSH
- úvodníky MeSH
- Geografické názvy
- Československo MeSH
- Maryland MeSH
- O autorovi
- Rous, Francis Payton, 1879-1970 Autorita
UNLABELLED: Comparing the gene expression profiles of metastatic and nonmetastatic cells has the power to reveal candidate metastasis-associated genes, whose involvement in metastasis can be experimentally tested. In this study, differentially expressed genes were explored in the v-src-transformed metastatic cell line PR9692 and its nonmetastatic subclone PR9692-E9. First, the contribution of homeodomain only protein X (HOPX) in metastasis formation and development was assessed. HOPX-specific knockdown decreased HOPX expression in the nonmetastatic subclone and displayed reduced cell motility in vitro. Critically, HOPX knockdown decreased the in vivo metastatic capacity in a syngeneic animal model system. Genomic analyses identified a cadre of genes affected by HOPX knockdown that intersected significantly with genes previously found to be differentially expressed in metastatic versus nonmetastatic cells. Furthermore, 232 genes were found in both screens with at least a two-fold change in gene expression, and a number of high-confidence targets were validated for differential expression. Importantly, significant changes were demonstrated in the protein expression level of three metastatic-associated genes (NCAM, FOXG1, and ITGA4), and knockdown of one of the identified HOPX-regulated metastatic genes, ITGA4, showed marked inhibition of cell motility and metastasis formation. These data demonstrate that HOPX is a metastasis-associated gene and that its knockdown decreases the metastatic activity of v-src-transformed cells through altered gene expression patterns. IMPLICATIONS: This study provides new mechanistic insight into a HOPX-regulated metastatic dissemination signature.
- MeSH
- buněčný cyklus MeSH
- down regulace MeSH
- experimentální sarkom genetika patologie sekundární MeSH
- forkhead transkripční faktory genetika metabolismus MeSH
- genový knockdown MeSH
- geny src MeSH
- homeodoménové proteiny genetika metabolismus MeSH
- kur domácí MeSH
- metastázy nádorů genetika MeSH
- molekuly buněčné adheze nervové genetika metabolismus MeSH
- nádorová transformace buněk genetika MeSH
- nádorové buněčné linie MeSH
- pohyb buněk MeSH
- ptačí proteiny genetika metabolismus MeSH
- regulace genové exprese u nádorů MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
- stanovení celkové genové exprese MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This chapter provides a personal insight into the scientific and social atmosphere in former Czechoslovakia. It covers the period of the rise of Hasek's immunologic school and application of immunologic tolerance to Rous sarcoma virus (RSV) heterotransmission. These approaches permitted establishment of a new model of mammalian cells transformed by RSV (virogenic XC cells), where the noninfectious viral genome was kept indefinitely as new genetic information (provirus). RSV was rescued from nonpermissive mammalian cells by fusion (complementation) with permissive chicken fibroblasts; this opened the way to understanding virus nonpermissiveness. Mammalian cells transformed by the reverse transcript of v-src mRNA were characterized, and the resulting provirus was shown to be highly oncogenic for chickens and to carry tumor-specific transplantation antigen. Other areas covering epigenetic reversion of RSV-transformed cells and long-term persistence of chicken leucosis viruses in foreign avian species are discussed.
- MeSH
- dějiny 20. století MeSH
- financování organizované MeSH
- geny src MeSH
- integrace viru MeSH
- lékařská onkologie dějiny MeSH
- onkogenní viry fyziologie MeSH
- virová transformace buněk MeSH
- virus Rousova sarkomu fyziologie MeSH
- výzkum MeSH
- zvířata MeSH
- Check Tag
- dějiny 20. století MeSH
- zvířata MeSH
- Publikační typ
- historické články MeSH
- Geografické názvy
- Československo MeSH
