Azo dyes are used as coloring agent in textile industries at larger scale. As a result, large quantity of dye-enriched waste water is generated which subsequently poses environmental problems. Biological tool involving bacteria having azoreductase enzyme has proved to be more effective and efficient in dye effluent treatment. Current work focuses on Staphylococcus caprae (S. caprae) for degradation and decolorization of Reactive Red-195 (RR-195) azo dye. For this purpose, factors such as pH, temperature, inoculums, carbon and nitrogen sources, and dye concentrations have been optimized for maximum decolorization and degradation. S. caprae (4 mg/mL) efficiently resulted into 90% decolorization of RR-195 dye under static condition at 100 μg/mL concentration, 30 °C and pH 7.0 at a 12-h contact period. FTIR analysis has revealed the formation of new functional groups in the treated dye such as O-H stretch at 3370 cm-1, C-H band stretching at 2928 cm-1, and new band at 1608 cm-1 which specify the degradation of aromatic ring, 1382 and 1118 cm-1 represents desulfonated peaks. Biodegraded metabolites of RR-195 dye such as phenol, 3, 5-di-tert-butylphenol, and phthalic acid have been identified respectively that find industrial applications. Phytotoxicity test has shown non-toxic effects of treated dye on germination of Vigna radiata and Triticum aestivum seeds. Further, antibiotic diffusion assay has confirmed the biosafety of S. caprae.
- MeSH
- azosloučeniny * metabolismus toxicita MeSH
- barvicí látky * metabolismus MeSH
- biodegradace * MeSH
- chemické látky znečišťující vodu metabolismus MeSH
- dusík metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- odpadní voda * mikrobiologie chemie MeSH
- průmyslový odpad MeSH
- Staphylococcus capitis metabolismus izolace a purifikace MeSH
- Staphylococcus metabolismus MeSH
- teplota MeSH
- textilie MeSH
- textilní průmysl MeSH
- uhlík metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
Byl popsán nový druh koaguláza pozitivního stafylokoka, který byl izolován z humánního klinického materiálu. Staphylococcus cornubiensis je fenotypově téměř shodný s druhem S. pseudintermedius (patří do skupiny „SIG“ – S. intermedius group), genotypově je ale od zástupců této skupiny odlišný. Zatím byl izolován jeden kmen z kůže 64letého muže s celulitidou v ordinaci primární lékařské péče v Cornwallu ve Spojeném království.
A novel species of coagulase-positive Staphylococcus isolated from human clinical material has been described. It is named Staphylococcus cornubiensis and is phenotypically highly similar to Staphylococcus pseudintermedius (from the S. intermedius group, SIG) but differs genotypically from the SIG members. A single strain has been isolated so far from the skin of a 64-year-old man with cellulitis in a primary health care service setting in Cornwall, UK.
- Klíčová slova
- Staphylococcus cornubiensis,
- MeSH
- celulitis diagnóza etiologie mikrobiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- stafylokokové infekce * diagnóza mikrobiologie MeSH
- Staphylococcus * izolace a purifikace klasifikace metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- tabulky MeSH
In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococci from ostriches and pheasants and their sensitivity to lantibiotic bacteriocin gallidermin. Gallidermin belongs in a group of polycyclic proteinaceous antimicrobial substances. Thirty-six faecal staphylococci (24 strains from 140 ostriches, 12 from 60 pheasants) comprising different species were tested. Staphylococci from ostriches and pheasants did not produce tryptamine-TRYP, putrescine-PUT, cadaverine-CAD or histamine-HIS. Production of tyramine-TYM, phenylethylamine-PEA was high or very high (100-1000 mg/L). Production of spermine-SPM and spermidine-SPD by staphylococci was very low or low although in the case of staphylococci from pheasants medium production of SPM was found. Because of the risk posed by BAs for consumers, the control of BA-producing bacteria is important from the points of view not only of safety assessment of food-producing animals but also of human health safety. The sensitivity to gallidermin in biogenic amine-producing staphylococci from ostriches and pheasants detected here is the most promising indication for further application of gallidermin for veterinary purposes. The novelty of our study lies in testing the ability of faecal staphylococci from ostriches and pheasants to produce BAs and in their treatment with gallidermin which has so far not been tested in this way.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriociny farmakologie MeSH
- biogenní aminy metabolismus MeSH
- feces mikrobiologie MeSH
- Galliformes mikrobiologie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- peptidy farmakologie MeSH
- Staphylococcus účinky léků izolace a purifikace metabolismus MeSH
- Struthioniformes mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Thirteen coagulase-negative, oxidase-negative, and novobiocin-susceptible staphylococci were isolated from human clinical specimens. The isolates were differentiated from known staphylococcal species on the basis of 16S rRNA, hsp60, rpoB, dnaJ, tuf, and gap gene sequencing, automated ribotyping, (GTG)5-PCR fingerprinting, and MALDI-TOF MS analysis. Phylogenetic analysis based on the 16S rRNA gene sequence indicated phylogenetic relatedness of the analyzed strains to Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus devriesei, and Staphylococcus lugdunensis. DNA-DNA hybridization experiments between representative strains CCM 8418(T), CCM 8421(T), and the closest phylogenetic neighbors confirmed that the isolates represent novel Staphylococcus species, for which the name Staphylococcus petrasii sp. nov. is proposed. Genotypic and phenotypic analyses unambiguously split the strains into two closely related subclusters. Based on the results, two novel subspecies S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov. are proposed, with type strains CCM 8418(T) (=CCUG 62727(T)) and CCM 8421(T) (=CCUG 62728(T)), respectively.
- MeSH
- antibakteriální látky metabolismus MeSH
- bakteriální proteiny genetika MeSH
- DNA bakterií chemie genetika MeSH
- fylogeneze MeSH
- hybridizace nukleových kyselin MeSH
- koagulasa metabolismus MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- novobiocin metabolismus MeSH
- oxidoreduktasy metabolismus MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus klasifikace genetika izolace a purifikace metabolismus MeSH
- techniky typizace bakterií MeSH
- ucho mikrobiologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of the study was to explore production of seven biogenic amines (phenylethylamine, histamine, cadaverine, tyramine, putrescine, spermine and/or spermidine) by selected staphylococci and enterococci. Thirty three enterococcal strains isolated from rabbit meat (Oryctolagus cuniculus f. domesticus) and 21 staphylococcal strains isolated from intestinal content of trout (Salmo trutta morpha fario) were tested. Production of biogenic amines was evaluated after cultivation of the tested microorganisms in the de Man, Rogosa and Sharpe Broth (enterococci) or in the Brain Heart Infusion Broth (staphylococci). Both the above cultivation media were enriched with selected amino acids (histidine, tyrosine, arginine, ornithine and lysine; 2g/L each) serving as precursors of biogenic amines. After cultivation, levels of the monitored biogenic amines in broths were analysed by a high performance liquid chromatography equipped with a UV/VIS DAD detector. Among 21 staphylococci, 18 strains produced tyramine or cadaverine, 13 strains formed putrescine or phenylethylamine and only one strain generated histamine. Two staphylococcal strains produced cadaverine levels above 1000 mg/L. Among 33 enterococcal strains, 27 formed cadaverine, 18 strains produced tyramine, 10 strains generated phenylethylamine, and 2 strains gave putrescine. Most of the tyramine producing enterococci generated more than 1000 mg/L of this biogenic amine. Production of spermine or spermidine by the studied strains was not proved.
- MeSH
- biogenní aminy analýza biosyntéza MeSH
- dekarboxylace MeSH
- Enterococcus klasifikace růst a vývoj izolace a purifikace metabolismus MeSH
- králíci * MeSH
- maso mikrobiologie MeSH
- pstruh * MeSH
- Staphylococcus klasifikace růst a vývoj izolace a purifikace metabolismus MeSH
- střeva mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- králíci * MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- fenotyp MeSH
- lidé MeSH
- mnohočetná léková rezistence MeSH
- novobiocin MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus klasifikace metabolismus účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
73 l. : il. ; 32 cm
Studium charakteristik kmenů koaguláza-negativních stafylokoků,dosud spíše opomíjených infekčních původců,které se stále častěji uplatňují jako etiologická agens řady vážných onemocnění,především u imunokompromitovaných pacientů (př.smrtelné sepse).
- Konspekt
- Mikrobiologie
- NLK Obory
- mikrobiologie, lékařská mikrobiologie
- NLK Publikační typ
- závěrečné zprávy o řešení grantu IGA MZ ČR
