Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20mg fresh weight, FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA.

• MeSH
• chromatografie kapalinová MeSH
• cyklopentany analýza   chemie   MeSH
• extrakce na pevné fázi MeSH
• fyziologický stres MeSH
• kyselina abscisová analýza   chemie   MeSH
• kyselina salicylová analýza   chemie   MeSH
• kyseliny indoloctové analýza   MeSH
• listy rostlin chemie   MeSH
• mastné kyseliny analýza   chemie   MeSH
• molekulární struktura MeSH
• oxylipiny analýza   chemie   MeSH
• regulátory růstu rostlin chemie   izolace a purifikace   fyziologie   MeSH
• tandemová hmotnostní spektrometrie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH