Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Förster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Förster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (λex 400 nm). The saturation curve of sarcosine based on FRET efficiency (F₆₀₄/F₅₁₀ ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.

• MeSH
• dextrany chemie   ultrastruktura   MeSH
• lidé MeSH
• magnetické nanočástice chemie   ultrastruktura   MeSH
• molekulární zobrazování metody   MeSH
• monoklonální protilátky chemie   imunologie   MeSH
• nádorové biomarkery analýza   MeSH
• nádorové buněčné linie MeSH
• nádory prostaty chemie   diagnóza   imunologie   MeSH
• nanokapsle chemie   ultrastruktura   MeSH
• reprodukovatelnost výsledků MeSH
• rezonanční přenos fluorescenční energie metody   MeSH
• sarkosin analýza   imunologie   MeSH
• senzitivita a specificita MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH