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Paramagnetic nanoparticles as a platform for FRET-based sarcosine picomolar detection
Z. Heger, N. Cernei, S. Krizkova, M. Masarik, P. Kopel, P. Hodek, O. Zitka, V. Adam, R. Kizek,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Directory of Open Access Journals
od 2011
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Nature Open Access
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PubMed Central
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od 2011-01-01
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od 2011-01-01
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od 2011-01-01
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Springer Nature OA/Free Journals
od 2011-12-01
PubMed
25746688
DOI
10.1038/srep08868
Knihovny.cz E-zdroje
- MeSH
- dextrany chemie ultrastruktura MeSH
- lidé MeSH
- magnetické nanočástice chemie ultrastruktura MeSH
- molekulární zobrazování metody MeSH
- monoklonální protilátky chemie imunologie MeSH
- nádorové biomarkery analýza MeSH
- nádorové buněčné linie MeSH
- nádory prostaty chemie diagnóza imunologie MeSH
- nanokapsle chemie ultrastruktura MeSH
- reprodukovatelnost výsledků MeSH
- rezonanční přenos fluorescenční energie metody MeSH
- sarkosin analýza imunologie MeSH
- senzitivita a specificita MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Förster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Förster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (λex 400 nm). The saturation curve of sarcosine based on FRET efficiency (F₆₀₄/F₅₁₀ ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.
Citace poskytuje Crossref.org
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- $a Heger, Zbynek $u 1] Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00, Czech Republic, European Union [2] Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic, European Union.
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- $a Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Förster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Förster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (λex 400 nm). The saturation curve of sarcosine based on FRET efficiency (F₆₀₄/F₅₁₀ ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.
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