In my article I tried to present the results of early experiments suggesting a significant role for cell association in Rous sarcoma virus transformation of non-permissive cells and revealing that infectious virus can be efficiently rescued from such cells by their fusion with permissive chicken fibroblasts.

• MeSH
• krysa rodu rattus MeSH
• kur domácí virologie   MeSH
• proviry patogenita   fyziologie   MeSH
• ptačí sarkom virologie   MeSH
• replikace viru MeSH
• virová transformace buněk MeSH
• virus Rousova sarkomu patogenita   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

This article summarizes the essential steps in understanding the chicken Rous sarcoma virus (RSV) genome association with a nonpermissive rodent host cell genome. This insight was made possible by in-depth study of RSV-transformed rat XC cells, which were called virogenic because they indefinitely carry virus genetic information in the absence of any infectious virus production. However, the virus was rescued by association of XC cells with chicken fibroblasts, allowing cell fusion between both partners. This and additional studies led to the interpretation that the RSV genome gets integrated into the host cell genome as a provirus. Study of additional rodent virogenic cell lines provided evidence that the transcript of oncogene v-src can be transmitted to other retroviruses and produce cell transformation by itself. As discussed in the text, two main questions related to nonpermissiveness to retrovirus infection remain to be solved. The first is changes in the retrovirus envelope gene allowing virus entry into a nonpermissive cell. The second is the nature of the permissive cell functions required by the nonpermissive cell to ensure infectious virus production. Both lines of investigation are being pursued.

• MeSH
• buněčné linie MeSH
• fúze buněk * MeSH
• genom virový genetika   MeSH
• genové produkty env genetika   MeSH
• krysa rodu rattus MeSH
• kur domácí virologie   MeSH
• onkogenní protein pp60(v-src) genetika   MeSH
• proviry genetika   růst a vývoj   MeSH
• virová transformace buněk MeSH
• virus Rousova sarkomu genetika   růst a vývoj   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH