The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.

• MeSH
• Arabidopsis genetika   metabolismus   MeSH
• biotechnologie MeSH
• cytokininy metabolismus   MeSH
• extracelulární prostor metabolismus   MeSH
• geneticky modifikované rostliny MeSH
• intracelulární prostor metabolismus   MeSH
• ječmen (rod) růst a vývoj   metabolismus   MeSH
• kompartmentace buňky MeSH
• listy rostlin metabolismus   MeSH
• membránové transportní proteiny genetika   metabolismus   MeSH
• mutace MeSH
• proteiny huseníčku genetika   metabolismus   MeSH
• protoplasty metabolismus   MeSH
• regulátory růstu rostlin metabolismus   MeSH
• vakuoly metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH