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Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots

E. Jiskrová, O. Novák, H. Pospíšilová, K. Holubová, M. Karády, P. Galuszka, S. Robert, I. Frébort,

. 2016 ; 33 (5 Pt B) : 735-42. [pub] 20160108

Language English Country Netherlands

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc17014157

The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.

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$a Jiskrová, Eva $u Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
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$a Extra- and intracellular distribution of cytokinins in the leaves of monocots and dicots / $c E. Jiskrová, O. Novák, H. Pospíšilová, K. Holubová, M. Karády, P. Galuszka, S. Robert, I. Frébort,
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$a The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.
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$a Novák, Ondřej $u Department of Metabolomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
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$a Pospíšilová, Hana $u Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
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$a Holubová, Katarína $u Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
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$a Karády, Michal $u Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic; Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå SE-90183, Sweden.
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$a Galuszka, Petr $u Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.
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$a Robert, Stéphanie $u Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå SE-90183, Sweden.
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$a Frébort, Ivo $u Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic. Electronic address: ivo.frebort@upol.cz.
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