30276834 OR Amniotic fluid cell-free DNA in preterm prelabor rupture of membranes Dotaz Zobrazit nápovědu
INTRODUCTION: We evaluated the levels of cell-free nuclear DNA (nDNA) and cell-free mitochondrial DNA (mtDNA) in the amniotic fluid supernatant from pregnancies complicated by preterm prelabor rupture of membranes (PPROM) based on evidence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). MATERIAL AND METHODS: A total of 155 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. The levels of cell-free nDNA and mtDNA in the amniotic fluid supernatant were assessed and quantified by real-time polymerase chain reaction. RESULTS: The levels of cell-free nDNA and mtDNA were higher in women with MIAC and IAI than in women without these conditions (nDNA: with MIAC: median 3.9 × 104 genome equivalent [GE]/mL vs without MIAC: median 1.2 × 104 GE/mL, with IAI: median: 5.3 × 104 GE/mL vs without IAI: median 1.2 × 104 GE/mL; mtDNA: with MIAC: median 9.2 × 105 GE/mL vs without MIAC: median 2.5 × 105 GE/mL, with IAI: median 1.1 × 106 GE/mL vs without IAI: median 2.5 × 105 ; all P values ≤ 0.01). Women with the microbial-associated IAI showed the highest levels of cell-free nDNA and mtDNA. CONCLUSIONS: Cell-free nDNA and mtDNA are constituents of the amniotic fluid supernatant from PPROM pregnancies. Both cell-free nDNA and mtDNA are involved in the intra-amniotic inflammatory response in women with PPROM.
- MeSH
- amniocentéza MeSH
- bakteriální infekce metabolismus MeSH
- Chlamydia trachomatis MeSH
- chorioamnionitida metabolismus MeSH
- dospělí MeSH
- gestační stáří MeSH
- interleukin-6 metabolismus MeSH
- kohortové studie MeSH
- kultivační techniky MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé MeSH
- mitochondriální DNA metabolismus MeSH
- Mycoplasma hominis MeSH
- plodová voda metabolismus mikrobiologie MeSH
- polymerázová řetězová reakce MeSH
- předčasný odtok plodové vody metabolismus MeSH
- retrospektivní studie MeSH
- RNA ribozomální 16S analýza MeSH
- těhotenství MeSH
- Ureaplasma MeSH
- volné cirkulující nukleové kyseliny metabolismus MeSH
- zánět metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
