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Austrian Agency for Health and Food S... 1 Department of Food Engineering Univer... 1 Department of Nutrition and Public He... 1 FoodMicrobe com Ltd Adams Hill Keywor... 1 Intestinal Bacteriology Research Labo... 1 Ptacy s r o Valasska Bystrice 194 756... 1 Science and Research Center Faculty o... 1
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Austrian Agency for Health and Food S... 1 Department of Food Engineering Univer... 1 Department of Nutrition and Public He... 1 FoodMicrobe com Ltd Adams Hill Keywor... 1 Intestinal Bacteriology Research Labo... 1 Ptacy s r o Valasska Bystrice 194 756... 1 Science and Research Center Faculty o... 1
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PubMed Central od 2012
Europe PubMed Central od 2012
ProQuest Central od 2012-01-01
Open Access Digital Library od 2012-01-01
Open Access Digital Library od 2012-01-01
ROAD: Directory of Open Access Scholarly Resources od 2012
PubMed
37237754
DOI
10.3390/antibiotics12050851
Knihovny.cz E-zdroje
The objective of this study was to use whole-genome sequencing (WGS) to screen for genes encoding for antibiotic resistance, fitness and virulence in Cronobacter sakazakii strains that had been isolated from food and powdered-milk-producing environments. Virulence (VGs) and antibiotic-resistance genes (ARGs) were detected with the Comprehensive Antibiotic Resistance Database (CARD) platform, ResFinder and PlasmidFinder tools. Susceptibility testing was performed using disk diffusion. Fifteen presumptive strains of Cronobacter spp. were identified by MALDI-TOF MS and ribosomal-MLST. Nine C. sakazakii strains were found in the meningitic pathovar ST4: two were ST83 and one was ST1. The C. sakazakii ST4 strains were further distinguished using core genome MLST based on 3678 loci. Almost all (93%) strains were resistant to cephalotin and 33% were resistant to ampicillin. In addition, 20 ARGs, mainly involved in regulatory and efflux antibiotics, were detected. Ninety-nine VGs were detected that encoded for OmpA, siderophores and genes involved in metabolism and stress. The IncFIB (pCTU3) plasmid was detected, and the prevalent mobile genetic elements (MGEs) were ISEsa1, ISEc52 and ISEhe3. The C. sakazakii isolates analyzed in this study harbored ARGs and VGs, which could have contributed to their persistence in powdered-milk-producing environments, and increase the risk of infection in susceptible population groups.
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Po ukončení testovacího provozu bude odkaz přesměrován adresu produkční verze portálu Medvik.