Pig is a domestic species of major importance in the agro-economy and in biomedical research. Mononuclear phagocytes (MNP) are organized in subsets with specialized roles in the orchestration of the immune response and new tools are awaited to improve MNP subset identification in the pig. We cloned pig CD11c cDNA and generated a monoclonal antibody to pig CD11c which showed a pattern of expression by blood and skin MNP subsets similar to humans. We also developed a porcine XCL1-mCherry dimer which specifically reacted with the XCR1-expressing dendritic cell subset of the type 1 lineage in blood and skin. These original reagents will allow the efficient identification of pig MNP subsets to study their role in physiological and pathological processes and also to target these cells in novel intervention and vaccine strategies for veterinary applications and preclinical evaluations.
- MeSH
- antigeny CD11c imunologie metabolismus MeSH
- dendritické buňky fyziologie MeSH
- fagocyty imunologie MeSH
- imunologické testy metody MeSH
- klonování DNA MeSH
- kůže metabolismus MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- monoklonální protilátky metabolismus MeSH
- mononukleární fagocytární systém * MeSH
- prasata imunologie MeSH
- receptory spřažené s G-proteiny imunologie metabolismus MeSH
- veterinární lékařství MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
