The anoxygenic phototrophic bacteria (APB) are an active component of aquatic microbial communities. While DNA-based studies have delivered a detailed picture of APB diversity, they cannot provide any information on the activity of individual species. Therefore, we focused on the expression of a photosynthetic gene by APB communities in two freshwater lakes (Cep lake and the Římov Reservoir) in the Czech Republic. First, we analyzed expression levels of pufM during the diel cycle using RT-qPCR. The transcription underwent a strong diel cycle and was inhibited during the day in both lakes. Then, we compared DNA- (total) and RNA-based (active) community composition by sequencing pufM amplicon libraries. We observed large differences in expression activity among different APB phylogroups. While the total APB community in the Římov Reservoir was dominated by Betaproteobacteria, Alphaproteobacteria prevailed in the active library. A different situation was encountered in the oligotrophic lake Cep where Betaproteobacteria (order Burkholderiales) dominated both the DNA and RNA libraries. Interestingly, in Cep lake we found smaller amounts of highly active uncultured phototrophic Chloroflexi, as well as phototrophic Gemmatimonadetes. Despite the large diversity of APB communities, light repression of pufM expression seems to be a common feature of all aerobic APB present in the studied lakes.
- MeSH
- Alphaproteobacteria izolace a purifikace fyziologie účinky záření MeSH
- bakteriální proteiny genetika metabolismus MeSH
- Betaproteobacteria izolace a purifikace fyziologie účinky záření MeSH
- DNA bakterií genetika izolace a purifikace MeSH
- fotoperioda * MeSH
- fotosyntetické reakční centrum - proteinové komplexy genetika metabolismus MeSH
- fototrofní procesy genetika účinky záření MeSH
- fylogeneze MeSH
- jezera mikrobiologie MeSH
- mikrobiota fyziologie účinky záření MeSH
- regulace genové exprese u bakterií fyziologie účinky záření MeSH
- světlo škodlivé účinky MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
