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Autor
Bobál, P 2 Strouhalová, D 2 Bobálová, J 1 Bobáľ, P 1 Bobáľová, Janette 1 Brtko, Július 1 Chalupová, Marta 1 Horská, Kateřina 1 Horváthová, Eva 1 Hunáková, Ľubica 1 Klusáková, J 1 Kružicová, A 1 Kuzmínová, G 1 Laštovičková, M 1 Macejová, Dana 1 Mosná, B 1 Otevřel, J 1 Pížová, H 1 Račanská, Eva 1 Suchý, Pavel 1
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Pracoviště
Cancer Research Institute BMC Univers... 1 Department of Chemical Drugs Faculty ... 1 Department of Chemical Drugs Faculty ... 1 Department of Chemical Drugs Universi... 1 Department of Human Pharmacology and ... 1 Department of NaturalDruags Universit... 1 Department of Pharmacology and Toxico... 1 Institute of Analytical Chemistry of ... 1 Institute of Analytical Chemistry of ... 1 Institute of Experimental Endocrinolo... 1 MDgK spol s r o Brno Czech Republic 1
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Autor
Bobál, P 2 Strouhalová, D 2 Bobálová, J 1 Bobáľ, P 1 Bobáľová, Janette 1 Brtko, Július 1 Chalupová, Marta 1 Horská, Kateřina 1 Horváthová, Eva 1 Hunáková, Ľubica 1 Klusáková, J 1 Kružicová, A 1 Kuzmínová, G 1 Laštovičková, M 1 Macejová, Dana 1 Mosná, B 1 Otevřel, J 1 Pížová, H 1 Račanská, Eva 1 Suchý, Pavel 1
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Pracoviště
Cancer Research Institute BMC Univers... 1 Department of Chemical Drugs Faculty ... 1 Department of Chemical Drugs Faculty ... 1 Department of Chemical Drugs Universi... 1 Department of Human Pharmacology and ... 1 Department of NaturalDruags Universit... 1 Department of Pharmacology and Toxico... 1 Institute of Analytical Chemistry of ... 1 Institute of Analytical Chemistry of ... 1 Institute of Experimental Endocrinolo... 1 MDgK spol s r o Brno Czech Republic 1
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E-zdroje NLK Online
Free Medical Journals od 2008PubMed Central od 2008 do 2019
ProQuest Central od 2008-06-01 do 2019-01-31
Open Access Digital Library od 2008-01-01
Open Access Digital Library od 2008-01-01
Open Access Digital Library od 2009-06-19
Nursing & Allied Health Database (ProQuest) od 2008-06-01 do 2019-01-31
Health & Medicine (ProQuest) od 2008-06-01 do 2019-01-31
Public Health Database (ProQuest) od 2008-06-01 do 2019-01-31
Sciendo od 2009-06-19
ROAD: Directory of Open Access Scholarly Resources od 2008
- Publikační typ
- abstrakt z konference MeSH
E-zdroje NLK Online
Free Medical Journals od 2008PubMed Central od 2008 do 2019
ProQuest Central od 2008-06-01 do 2019-01-31
Open Access Digital Library od 2008-01-01
Open Access Digital Library od 2008-01-01
Open Access Digital Library od 2009-06-19
Nursing & Allied Health Database (ProQuest) od 2008-06-01 do 2019-01-31
Health & Medicine (ProQuest) od 2008-06-01 do 2019-01-31
Public Health Database (ProQuest) od 2008-06-01 do 2019-01-31
Sciendo od 2009-06-19
ROAD: Directory of Open Access Scholarly Resources od 2008
- Publikační typ
- abstrakt z konference MeSH
PubMed
30660590
DOI
10.1016/j.ab.2019.01.004
Knihovny.cz E-zdroje
The main objective of this study was to develop an effective in-gel trypsin digestion protocol using aqueous-acetonitrile solvent system to facilitate MS analysis and maximize the number of identified proteins from biological samples. The procedure, where 80% acetonitrile was present in the trypsin reaction mixture, increased the number of matched peptides, and allowed the identification of more proteins with higher coverage than the common digestion protocol. Vimentin, annexins, tubulin, actin, peptidyl-prolyl cis-trans isomerase or alpha-enolase are examples of important proteins that change during the progress cancer. These were isolated from human breast cancer cells and were used for this study.
- MeSH
- acetonitrily chemie MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádorové proteiny analýza izolace a purifikace metabolismus MeSH
- nádory metabolismus patologie MeSH
- peptidy analýza MeSH
- proteomika metody MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- trypsin metabolismus MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
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Název dokumentu
Po ukončení testovacího provozu bude odkaz přesměrován adresu produkční verze portálu Medvik.