The molecular weight (Mw) of dextran derivatives, such as regioselectively oxidized dicarboxydextran (DXA), is greatly influencing their faith in an organism, which could be possibly used to improve anticancer drug delivery. Here we present a modified method of sulfonation-induced chain scission allowing direct and accurate control over the Mw of DXA without increasing its polydispersity. Prepared DXA derivatives (Mw = 10-185 kDa) have been conjugated to cisplatin and the Mw of the carrier found to have a significant impact on cisplatin release rates, in vitro cytotoxicity, and migrastatic potential. Conjugates with the high-Mw DXA showed particularly increased anticancer efficacy. The best conjugate was four times more effective against malignant prostatic cell lines than free cisplatin and significantly inhibited the ovarian cancer cell migration. This was traced to the characteristics of spontaneously formed cisplatin-crosslinked DXA nanogels influenced by Mw of DXA and amount of loaded cisplatin.
- MeSH
- adenokarcinom farmakoterapie metabolismus MeSH
- antitumorózní látky chemie farmakologie MeSH
- buňky A549 MeSH
- cisplatina chemie farmakologie MeSH
- dextrany chemie MeSH
- lidé MeSH
- molekulová hmotnost MeSH
- nádory prostaty farmakoterapie metabolismus MeSH
- nádory vaječníků farmakoterapie metabolismus MeSH
- nádory farmakoterapie metabolismus MeSH
- nanogely chemie MeSH
- nosiče léků chemie MeSH
- oxidace-redukce MeSH
- pohyb buněk účinky léků MeSH
- systémy cílené aplikace léků metody MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Publikační typ
- abstrakt z konference MeSH
