Ulcerative colitis and Crohn's disease constitute the two main forms of inflammatory bowel disease. Prevalence of these diseases increases. In the present day, inadequate and inefficient therapy causes complications and frequent relapse. Extracellular DNA (ecDNA) is the DNA that is outside of cells and may be responsible for activation of the inflammatory response. To determine whether colitis is associated with higher concentration of ecDNA we used male mice of the C57BL/6 strain. Colitis was induced by 2% dextran sulphate sodium (DSS). After 7 days, mice exhibited considerable weight loss compared to the control group. Also, there was a higher stool consistency score and the colon was significantly shorter in comparison to the control group. Higher concentration of ecDNA was found in the DSS group. Interestingly, deoxyribonuclease activity was lower in the colon of the DSS group compared with the negative control. These findings may point to ecDNA as a potential pathogenetic factor and marker of inflammation.
- MeSH
- biologické markery metabolismus MeSH
- deoxyribonukleasy metabolismus MeSH
- DNA krev metabolismus MeSH
- gastrointestinální trakt enzymologie MeSH
- kolitida krev metabolismus patologie MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední C57BL MeSH
- síran dextranu MeSH
- tělesná hmotnost MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Autorky podávají přehled o možnostech screeningových vyšetření prováděných na novorozeneckých oddělení.
The authors present an overview of available screening examinations performed at neonate departments.
- MeSH
- antidota klasifikace terapeutické užití MeSH
- diferenciální diagnóza MeSH
- jedovaté rostliny MeSH
- jedy chemie otrava MeSH
- lidé MeSH
- nežádoucí účinky léčiv etiologie patofyziologie terapie MeSH
- otrava diagnóza etiologie terapie MeSH
- první pomoc metody normy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
