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Autor
Colleoni, Silvia 1 Diecke, Sebastian 1 Drukker, Micha 1 Galli, Cesare 1 Göritz, Frank 1 Hayashi, Katsuhiko 1 Hayashi, Masafumi 1 Hermes, Robert 1 Hildebrandt, Thomas B 1 Holtze, Susanne 1 Izsvák, Zsuzsanna 1 Lazzari, Giovanna 1 Michel, Geert 1 Rishko, Valentyna 1 Ruiz-Orera, Jorge 1 Rusha, Ejona 1 Shaposhnikov, Dmitry 1 Stejskal, Jan 1 Telugu, Narasimha 1 Wang, Jichang 1
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Pracoviště
Cardiovascular and Metabolic Sciences... 1 Department of Developmental Genetics ... 1 Department of Stem Cell Biology and M... 1 Division of Drug Discovery and Safety... 1 FEMTransgenic Technologies Charité 13... 1 Faculty of Veterinary Medicine Freie ... 1 Fondazione Avantea 26100 Cremona Italy 1 Induced Pluripotent Stem Cell Core Fa... 1 Laboratory of Reproductive Technologi... 1 Leibniz Institute for Zoo and Wildlif... 1 Mobile DNA Max Delbrück Center for Mo... 1 Technology Platform Pluripotent Stem ... 1 ZOO Dvůr Králové Štefánikova 1029 544... 1
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Autor
Colleoni, Silvia 1 Diecke, Sebastian 1 Drukker, Micha 1 Galli, Cesare 1 Göritz, Frank 1 Hayashi, Katsuhiko 1 Hayashi, Masafumi 1 Hermes, Robert 1 Hildebrandt, Thomas B 1 Holtze, Susanne 1 Izsvák, Zsuzsanna 1 Lazzari, Giovanna 1 Michel, Geert 1 Rishko, Valentyna 1 Ruiz-Orera, Jorge 1 Rusha, Ejona 1 Shaposhnikov, Dmitry 1 Stejskal, Jan 1 Telugu, Narasimha 1 Wang, Jichang 1
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Pracoviště
Cardiovascular and Metabolic Sciences... 1 Department of Developmental Genetics ... 1 Department of Stem Cell Biology and M... 1 Division of Drug Discovery and Safety... 1 FEMTransgenic Technologies Charité 13... 1 Faculty of Veterinary Medicine Freie ... 1 Fondazione Avantea 26100 Cremona Italy 1 Induced Pluripotent Stem Cell Core Fa... 1 Laboratory of Reproductive Technologi... 1 Leibniz Institute for Zoo and Wildlif... 1 Mobile DNA Max Delbrück Center for Mo... 1 Technology Platform Pluripotent Stem ... 1 ZOO Dvůr Králové Štefánikova 1029 544... 1
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NLK
Directory of Open Access Journals
od 2011
Free Medical Journals
od 2011
Nature Open Access
od 2011-12-01
PubMed Central
od 2011
Europe PubMed Central
od 2011
ProQuest Central
od 2011-01-01
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od 2011-01-01
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Health & Medicine (ProQuest)
od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2011
PubMed
35260583
DOI
10.1038/s41598-022-07059-w
Knihovny.cz E-zdroje
The northern white rhinoceros (NWR) is probably the earth's most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro. To elucidate the regulation of pluripotency and differentiation of NWR PSCs, we generated iPSCs from a deceased NWR female using episomal reprogramming, and observed surprising similarities to human PSCs. NWR iPSCs exhibit a broad differentiation potency into the three germ layers and trophoblast, and acquire a naïve-like state of pluripotency, which is pivotal to differentiate PSCs into primordial germ cells (PGCs). Naïve culturing conditions induced a similar expression profile of pluripotency related genes in NWR iPSCs and human ESCs. Furthermore, naïve-like NWR iPSCs displayed increased expression of naïve and PGC marker genes, and a higher integration propensity into developing mouse embryos. As the conversion process was aided by ectopic BCL2 expression, and we observed integration of reprogramming factors, the NWR iPSCs presented here are unsuitable for gamete production. However, the gained insights into the developmental potential of both primed and naïve-like NWR iPSCs are fundamental for in future PGC-specification in order to rescue the species from extinction using cryopreserved somatic cells.
- MeSH
- buněčná diferenciace genetika MeSH
- indukované pluripotentní kmenové buňky * MeSH
- myši MeSH
- Perissodactyla genetika MeSH
- zárodečné buňky metabolismus MeSH
- zárodečné listy MeSH
- zvířata MeSH
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- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
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