Staphylococcus aureus ssp anaerobius strain S10 was isolated from an outbreak of sheep abscess disease. Sequence of the catalase gene of this strain showed 99% identity to the catalase gene (katB) sequence of the reference strain (S. aureus ssp. anaerobius strain MVF213) with mismatching of three base pairs. An important substitution located 1036 nucleotides upstream of the initiation codon from "C" in katB to "T" in the catalase gene of strain S10 originated a stop codon. The deduced protein (345 amino acids) is 105 amino acids shorter than that of katB. Partial sequence of the catalase gene of other 8 local isolates in addition to another reference strain (DSM 20714/ATCC 35844) revealed the same mutations in all local (African) strains, whereas the sequence of the reference (European) strain was typical to that of katB. Sequence of the catalase gene of S. aureus ssp. anaerobius strain S10 was deposited in GenBank under accession no. EU281993.
- MeSH
- absces epidemiologie mikrobiologie veterinární MeSH
- bakteriální proteiny genetika MeSH
- DNA bakterií genetika chemie MeSH
- epidemický výskyt choroby MeSH
- financování organizované MeSH
- katalasa genetika MeSH
- molekulární sekvence - údaje MeSH
- nemoci ovcí epidemiologie mikrobiologie MeSH
- nesmyslný kodon MeSH
- ovce MeSH
- polymorfismus genetický MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie nukleových kyselin MeSH
- stafylokokové infekce epidemiologie mikrobiologie veterinární MeSH
- Staphylococcus aureus enzymologie izolace a purifikace klasifikace MeSH
- substituce aminokyselin genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
