A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed. This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material. Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques. Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells. Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde. Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
- MeSH
- Candida enzymologie MeSH
- chitosan chemie MeSH
- enzymy imobilizované chemie MeSH
- fungální proteiny chemie MeSH
- imobilizované buňky cytologie metabolismus MeSH
- kyseliny polymethakrylové chemie MeSH
- lipasa chemie MeSH
- magnetismus * MeSH
- Saccharomyces cerevisiae cytologie metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
