The protective effect of Enterococcus faecium EFAL41 on chicken's caecum in relation to the TLR (TLR4 and TLR21) activation and production of luminal IgA challenged with Campylobacter jejuni CCM6191 was assessed. The activation of MIF, IFN-β, MD-2 and CD14 was followed-up after bacterial infection. Day-old chicks (40) were divided into four groups (n = 10): control (C), E. faecium AL41 (EFAL41), C. jejuni (CJ) and combined E. faecium AL41+C. jejuni (EFAL41+CJ). Relative mRNA expression of TLR4, TLR21 and CD14 was upregulated in the probiotic strain and infected (combined) group on day 4 and 7 post infection (p.i.). The caecal relative MD-2 mRNA expression was upregulated on day 4 p.i. in the EFAL41+CJ and CJ groups. MIF and IFN-β reached the highest levels in the combined groups on day 7 p.i. The concentration of the sIgA in intestinal flush was upregulated in EFAL41+CJ group on day 4 p.i. The results demonstrated that E. faecium EFAL41 probiotic strain can modulate the TLRs expression and modify the activation of MIF, IFN-β, MD-2 and CD14 molecules in the chickens caecum challenged with C. jejuni CCM 6191. The counts of EFAL41 were sufficient and high, similarly the counts of enterococci in both, caecum and faeces but without reduction of Campylobacter counts.

• MeSH
• antigeny CD14 genetika   imunologie   MeSH
• Campylobacter jejuni růst a vývoj   MeSH
• cékum imunologie   mikrobiologie   MeSH
• Enterococcus faecium růst a vývoj   imunologie   MeSH
• feces mikrobiologie   MeSH
• imunoglobulin A sekreční genetika   MeSH
• interakce hostitele a patogenu MeSH
• interferon beta genetika   imunologie   MeSH
• kampylobakterové infekce dietoterapie   imunologie   mikrobiologie   veterinární   MeSH
• kur domácí MeSH
• lymfocytární antigen 96 genetika   imunologie   MeSH
• nemoci drůbeže dietoterapie   genetika   imunologie   mikrobiologie   MeSH
• novorozená zvířata MeSH
• probiotika farmakologie   MeSH
• protein - isoformy genetika   imunologie   MeSH
• receptory imunologické genetika   imunologie   MeSH
• regulace genové exprese MeSH
• signální transdukce MeSH
• toll-like receptor 4 genetika   imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH