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Autor
Aggarwal, Abhi 1 Bergerson, Samuel J 1 Chen, Yang 1 Hanson, Timothy L 1 Hasseman, Jeremy P 1 Hoppa, Michael B 1 Ji, Xiang 1 Kleinfeld, David 1 Kloos, Marinus 1 Konnerth, Arthur 1 Liu, Rui 1 Looger, Loren L 1 Marvin, Jonathan S 1 Mohar, Boaz 1 Mohr, Manuel A 1 Patel, Ronak 1 Podgorski, Kaspar 1 Ralowicz, Amelia J 1 Reep, Daniel 1 Schreiter, Eric R 1
- Organizace
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Pracoviště
Allen Institute for Neural Dynamics S... 1 Department of Biological Sciences Dar... 1 Department of Biosystems Science and ... 1 Department of Physics University of C... 1 Department of Physiology 2nd Faculty ... 1 Howard Hughes Medical Institute Depar... 1 Institute of Neuroscience and Cluster... 1 Janelia Research Campus Howard Hughes... 1 Neurosciences Graduate Program Univer... 1 Section of Neurobiology University of... 1
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Autor
Aggarwal, Abhi 1 Bergerson, Samuel J 1 Chen, Yang 1 Hanson, Timothy L 1 Hasseman, Jeremy P 1 Hoppa, Michael B 1 Ji, Xiang 1 Kleinfeld, David 1 Kloos, Marinus 1 Konnerth, Arthur 1 Liu, Rui 1 Looger, Loren L 1 Marvin, Jonathan S 1 Mohar, Boaz 1 Mohr, Manuel A 1 Patel, Ronak 1 Podgorski, Kaspar 1 Ralowicz, Amelia J 1 Reep, Daniel 1 Schreiter, Eric R 1
- Organizace
-
Pracoviště
Allen Institute for Neural Dynamics S... 1 Department of Biological Sciences Dar... 1 Department of Biosystems Science and ... 1 Department of Physics University of C... 1 Department of Physiology 2nd Faculty ... 1 Howard Hughes Medical Institute Depar... 1 Institute of Neuroscience and Cluster... 1 Janelia Research Campus Howard Hughes... 1 Neurosciences Graduate Program Univer... 1 Section of Neurobiology University of... 1
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- Kategorie
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NLK
ProQuest Central
od 2004-10-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2004-10-01 do Před 1 rokem
PubMed
37142767
DOI
10.1038/s41592-023-01863-6
Knihovny.cz E-zdroje
The fluorescent glutamate indicator iGluSnFR enables imaging of neurotransmission with genetic and molecular specificity. However, existing iGluSnFR variants exhibit low in vivo signal-to-noise ratios, saturating activation kinetics and exclusion from postsynaptic densities. Using a multiassay screen in bacteria, soluble protein and cultured neurons, we generated variants with improved signal-to-noise ratios and kinetics. We developed surface display constructs that improve iGluSnFR's nanoscopic localization to postsynapses. The resulting indicator iGluSnFR3 exhibits rapid nonsaturating activation kinetics and reports synaptic glutamate release with decreased saturation and increased specificity versus extrasynaptic signals in cultured neurons. Simultaneous imaging and electrophysiology at individual boutons in mouse visual cortex showed that iGluSnFR3 transients report single action potentials with high specificity. In vibrissal sensory cortex layer 4, we used iGluSnFR3 to characterize distinct patterns of touch-evoked feedforward input from thalamocortical boutons and both feedforward and recurrent input onto L4 cortical neuron dendritic spines.
- MeSH
- kinetika MeSH
- kyselina glutamová * metabolismus MeSH
- myši MeSH
- nervový přenos * MeSH
- neurony fyziologie MeSH
- synapse fyziologie MeSH
- zvířata MeSH
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- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
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