Cardiolipin and phosphatidylglycerol are anionic phospholipids localized to the inner mitochondrial membrane. In this study, it is demonstrated by fluorescence and transmission electron microscopy that atp2.1pgs1Δ mutant mitochondria lacking anionic phospholipids contain fragmented and swollen mitochondria with a completely disorganized inner membrane. In the second part of this study, it was shown that the temperature sensitivity of the atp2.1pgs1Δ mutant was not suppressed by the osmotic stabilizer glucitol but by glucosamine, a precursor of chitin synthesis. The atp2.1pgs1Δ mutant was hypersensitive to Calcofluor White and caffeine, resistant to Zymolyase, but its sensitivity to caspofungin was the same as the strains with the standard PGS1 gene. The distribution of chitin in the mutant cell wall was impaired. The glucan level in the cell wall of the atp2.1pgs1Δ mutant was reduced by 4-8 %, but the level of chitin was almost double that in the wild-type strain. The cell wall of the atp2.1pgs1Δ mutant was about 20 % thinner than the wild type, but its morphology was not significantly altered.
- MeSH
- aerobióza MeSH
- buněčná stěna chemie ultrastruktura MeSH
- delece genu MeSH
- fluorescenční mikroskopie MeSH
- fosfolipidy nedostatek MeSH
- glukany analýza MeSH
- Kluyveromyces cytologie genetika růst a vývoj metabolismus MeSH
- mitochondrie ultrastruktura MeSH
- teplota MeSH
- transmisní elektronová mikroskopie MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- antifungální látky farmakologie MeSH
- fungální proteiny genetika metabolismus MeSH
- Kluyveromyces metabolismus růst a vývoj MeSH
- membránové transportní proteiny genetika metabolismus MeSH
- mnohočetná fungální léková rezistence MeSH
- regulace genové exprese u hub MeSH
- techniky in vitro MeSH
- transkripční faktory genetika metabolismus MeSH
- MeSH
- eukaryotické buňky genetika metabolismus ultrastruktura MeSH
- fosfolipidy biosyntéza MeSH
- geny hub fyziologie MeSH
- Kluyveromyces genetika růst a vývoj ultrastruktura MeSH
- mitochondriální DNA fyziologie MeSH
- mitochondrie genetika metabolismus ultrastruktura MeSH
- respirační komplex IV genetika MeSH
