Plum pox virus (PPV, family Potyviridae) is one of the most important viral pathogens of Prunus spp. causing considerable damage to stone-fruit industry worldwide. Among the PPV strains identified so far, only PPV-C, PPV-CR, and PPV-CV are able to infect cherries under natural conditions. Herein, we evaluated the pathogenic potential of two viral isolates in herbaceous host Nicotiana benthamiana. Significantly higher accumulation of PPV capsid protein in tobacco leaves infected with PPV-CR (RU-30sc isolate) was detected in contrast to PPV-C (BY-101 isolate). This result correlated well with the symptoms observed in the infected plants. To further explore the host response upon viral infection at the molecular level, a comprehensive proteomic profiling was performed. Using reverse-phase ultra-high-performance liquid chromatography followed by label-free mass spectrometry quantification, we identified 38 unique plant proteins as significantly altered due to the infection. Notably, the abundances of photosynthesis-related proteins, mainly from the Calvin-Benson cycle, were found more aggressively affected in plants infected with PPV-CR isolate than those of PPV-C. This observation was accompanied by a significant reduction in the amount of photosynthetic pigments extracted from the leaves of PPV-CR infected plants. Shifts in the abundance of proteins that are involved in stimulation of photosynthetic capacity, modification of amino acid, and carbohydrate metabolism may affect plant growth and initiate energy formation via gluconeogenesis in PPV infected N. benthamiana. Furthermore, we suggest that the higher accumulation of H2O2 in PPV-CR infected leaves plays a crucial role in plant defense and development by activating the glutathione synthesis.
- MeSH
- chlorofyl biosyntéza MeSH
- chromatografie s reverzní fází MeSH
- energetický metabolismus genetika MeSH
- fotosyntéza genetika MeSH
- genotyp MeSH
- glutathion biosyntéza MeSH
- hmotnostní spektrometrie MeSH
- interakce hostitele a patogenu genetika MeSH
- karotenoidy biosyntéza MeSH
- listy rostlin genetika metabolismus virologie MeSH
- nemoci rostlin genetika virologie MeSH
- oxidace-redukce MeSH
- peroxid vodíku metabolismus MeSH
- proteiny teplotního šoku klasifikace genetika metabolismus MeSH
- Prunus avium virologie MeSH
- regulace genové exprese u rostlin * MeSH
- rostlinné proteiny klasifikace genetika metabolismus MeSH
- slivoň švestka virologie MeSH
- tabák genetika metabolismus virologie MeSH
- virus šarky švestky klasifikace genetika růst a vývoj patogenita MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The complete genomic sequence of a new virus from cherry trees was determined. Its genome is 5857 nt long and resembles that of members of the genus Luteovirus in its genomic organization and nucleotide sequence. Based on the species demarcation criteria for luteoviruses, the virus represents a new luteovirus species. Furthermore, a 47-nt-long inverted repeat was found at the 3' end of its genome. The virus has been provisionally named cherry-associated luteovirus (ChALV) and is the fourth member of the family Luteoviridae reported to naturally infect woody plants.
- MeSH
- 3' přiléhající oblast DNA MeSH
- délka genomu MeSH
- fylogeneze * MeSH
- genom virový * MeSH
- Luteovirus klasifikace genetika izolace a purifikace MeSH
- mapování chromozomů MeSH
- nemoci rostlin virologie MeSH
- obrácené repetice MeSH
- otevřené čtecí rámce MeSH
- RNA virová genetika MeSH
- sekvence nukleotidů MeSH
- slivoň švestka virologie MeSH
- Publikační typ
- časopisecké články MeSH
Plant NAD(+)-dependent aminoaldehyde dehydrogenases (AMADHs, EC 1.2.1.19) belong to the family 10 of aldehyde dehydrogenases. They participate in the metabolism of polyamines or osmoprotectants. The enzymes are characterized by their broad substrate specificity covering ω-aminoaldehydes, aliphatic and aromatic aldehydes as well as nitrogen-containing heterocyclic aldehydes. The isoenzyme 1 from tomato (Solanum lycopersicum; SlAMADH1) oxidizes aliphatic aldehydes very efficiently and converts also furfural, its derivatives or benzaldehyde, which are present at low concentrations in alcoholic distillates such as fruit brandy. In this work, SlAMADH1 was examined as a bioanalytical tool for their detection. These aldehydes arise from fermentation processes or thermal degradation of sugars and their presence is related to health complications after consumption including nausea, emesis, sweating, decrease in blood pressure, hangover headache, among others. Sixteen samples of slivovitz (plum brandy) from local producers in Moravia, Czech Republic, were analyzed for their aldehyde content using a spectrophotometric activity assay with SlAMADH1. In all cases, there were oxidative responses observed when monitoring NADH production in the enzymatic reaction. Aldehydes in the distillate samples were also subjected to a standard determination using reversed-phase HPLC with spectrophotometric and tandem mass spectrometric detection after a derivatization with 2,4-dinitrophenylhydrazine. Results obtained by both methods were found to correlate well for a majority of the analyzed samples. The possible applicability of SlAMADH1 for the evaluation of aldehyde content in food and beverages has now been demonstrated.
- MeSH
- aldehydy škodlivé účinky analýza MeSH
- alkoholické nápoje škodlivé účinky analýza MeSH
- biotechnologie MeSH
- destilace MeSH
- izoenzymy metabolismus MeSH
- kinetika MeSH
- lidé MeSH
- ovoce chemie MeSH
- retinaldehydrogenasa metabolismus MeSH
- rostlinné proteiny metabolismus MeSH
- slivoň švestka chemie MeSH
- Solanum lycopersicum enzymologie MeSH
- tandemová hmotnostní spektrometrie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Protein sample preparation is a critical and an unsustainable step since it involves the use of tedious methods that usually require high amount of solvents. The development of new materials offers additional opportunities in protein sample preparation. This work explores, for the first time, the potential application of carboxylate-terminated carbosilane dendrimers to the purification/enrichment of proteins. Studies on dendrimer binding to proteins, based on protein fluorescence intensity and emission wavelengths measurements, demonstrated the interaction between carboxylate-terminated carbosilane dendrimers and proteins at all tested pH levels. Interactions were greatly affected by the protein itself, pH, and dendrimer concentration and generation. Especially interesting was the interaction at acidic pH since it resulted in a significant protein precipitation. Dendrimer-protein interactions were modeled observing stable complexes for all proteins. Carboxylate-terminated carbosilane dendrimers at acidic pH were successfully used in the purification/enrichment of proteins extracted from a complex sample. Graphical Abstract Images showing the growing turbidity of solutions containing a mixture of proteins (lysozyme, myoglobin, and BSA) at different protein:dendrimer ratios (1:0, 1:1, 1:8, and 1:20) at acidic pH and SDS-PAGE profiles of the corresponsing supernatants. Comparison of SDS-PAGE profiles for the pellets obtained during the purification of proteins present in a complex sample using a conventional "no-clean" method based on acetone precipitation and the proposed "greener" method using carboxylate-terminated carbosilane dendrimer at a 1:20 protein:dendrimer ratio.
- MeSH
- chemická precipitace MeSH
- dendrimery chemie MeSH
- koncentrace vodíkových iontů MeSH
- kyseliny karboxylové chemie MeSH
- muramidasa chemie izolace a purifikace MeSH
- myoglobin chemie izolace a purifikace MeSH
- rostlinné proteiny izolace a purifikace MeSH
- rozpouštědla MeSH
- sekundární struktura proteinů MeSH
- semena rostlinná chemie MeSH
- sérový albumin hovězí chemie izolace a purifikace MeSH
- silany chemie MeSH
- simulace molekulární dynamiky MeSH
- slivoň švestka chemie MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
