Defects in DNA single-strand break repair (SSBR) are linked with neurological dysfunction but the underlying mechanisms remain poorly understood. Here, we show that hyperactivity of the DNA strand break sensor protein Parp1 in mice in which the central SSBR protein Xrcc1 is conditionally deleted (Xrcc1Nes-Cre ) results in lethal seizures and shortened lifespan. Using electrophysiological recording and synaptic imaging approaches, we demonstrate that aberrant Parp1 activation triggers seizure-like activity in Xrcc1-defective hippocampus ex vivo and deregulated presynaptic calcium signalling in isolated hippocampal neurons in vitro. Moreover, we show that these defects are prevented by Parp1 inhibition or deletion and, in the case of Parp1 deletion, that the lifespan of Xrcc1Nes-Cre mice is greatly extended. This is the first demonstration that lethal seizures can be triggered by aberrant Parp1 activity at unrepaired SSBs, highlighting PARP inhibition as a possible therapeutic approach in hereditary neurological disease.
- Klíčová slova
- DNA strand break, XRCC1, neurodegeneration, poly(ADP-ribose) polymerase, seizures,
- MeSH
- DNA vazebné proteiny * genetika metabolismus MeSH
- DNA MeSH
- myši MeSH
- neurony metabolismus MeSH
- oprava DNA genetika MeSH
- poly(ADP-ribosa)polymerasa 1 genetika metabolismus MeSH
- vápník * MeSH
- záchvaty genetika MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA vazebné proteiny * MeSH
- DNA MeSH
- poly(ADP-ribosa)polymerasa 1 MeSH
- vápník * MeSH
