The use of knowledge from technetium radiochemistry (even from nuclear medicine applications) allows us to select an sorbent for 99mTc radionuclide sorption, which is hydroxyapatite. Using radioisotope indication, the 99mTcO₄- sorption process on synthetic hydroxyapatite was studied by the batch method in the presence of SnCl2 and FeSO4 reducing agents. The complexing organic ligands' effect on the 99mTcO₄- sorption under reducing conditions was investigated. In the presence of Sn2+ ions without the addition of organic ligand, the sorption percentage reached above 90% independently of the environment. In the presence of Fe2+ ions without the addition of organic ligand, the sorption of 99mTcO₄- was significantly lower and was at approximately 6%, depending on the concentration of Fe2+ ions in solution. The effect of complexing organic ligands on the 99mTcO₄- sorption on hydroxyapatite from the aqueous solution, acetate buffer and phosphate buffer decreases in the following order for Sn2+: oxalic acid > ethylenediaminetetraacetic acid > ascorbic acid. In the presence of Fe2+ ions without organic ligands, the sorption reached up to 15% depending on the composition of the solution. The addition of oxalic acid and ascorbic acid increased the sorption up to 80%. The ethylenediaminetetraacetic acid had no significant effect on the sorption of technetium on hydroxyapatite.

• Klíčová slova
• hydroxyapatite, organic ligands, radioactive waste, sorption, technetium-99,
• MeSH
• 99mTc-technecistan sodný * MeSH
• adsorpce MeSH
• EDTA MeSH
• hydroxyapatit chemie   MeSH
• indikátory a reagencie MeSH
• kyselina oxalová MeSH
• ligandy MeSH
• technecium * analýza   MeSH
• voda MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 99mTc-technecistan sodný * MeSH
• EDTA MeSH
• hydroxyapatit MeSH
• indikátory a reagencie MeSH
• kyselina oxalová MeSH
• ligandy MeSH
• technecium * MeSH
• voda MeSH

INTRODUCTION: Preoperative parathyroid imaging is inevitable part of focused parathyroid surgery. The aim of our study was assessment of parathyroid scintigraphy diagnostic accuracy regarding to size and metabolic parameters of hyperfunctioning parathyroid tissue. MATERIAL AND METHODS: Parathyroid scintigraphy for suspected primary hyperparathyroidism was performed in 95 patients during years 2015 and 2016. Of them, 75 patients with known clinical outcome (40 underwent surgery, 35 had documented laboratory follow-up) were further retrospectively evaluated. The performance of dual tracer 99mTc-pertechnetate and 99mTc-MIBI subtraction and dual-time-point 99mTc-MIBI imaging with SPECT/CT was analysed. Serum parathyroid hormone (PTH), calcaemia, ionized calcaemia and phosphataemia and ultrasound detected adenoma volume and largest diameter in false negative and true positive findings were compared using Mann-Whitney test. RESULTS: Sensitivity and specificity of parathyroid scintigraphy was 74.5% and 95.8%, respectively. NPV was 63.8% and PPV 97.4%. Hyperfunctioning parathyroid tissue detectability was almost significantly associated with hypophosphataemia and PTH levels. CONCLUSION: Parathyroid scintigraphy provides high sensitivity and superior specificity in parathyroid adenoma location, nevertheless the diagnostic accuracy tends to decline in smaller adenomas and in less metabolically active parathyroid tissue causing only subtle biochemical changes. 18F-Fluorocholine PET/CT or 3D SPECT/CT subtraction should be a reasonable option for those cases.

• Klíčová slova
• computed tomography, hypercalcemia, hyperparathyroidism, hypophosphatemia, parathyroid hormone, primary, single photon emission computed tomography, ultrasonography,
• MeSH
• 99mTc-technecistan sodný terapeutické užití   MeSH
• časové faktory MeSH
• lidé středního věku MeSH
• lidé MeSH
• paratyreoidea diagnostické zobrazování   patologie   MeSH
• prediktivní hodnota testů MeSH
• primární hyperparatyreóza diagnostické zobrazování   patologie   MeSH
• reprodukovatelnost výsledků MeSH
• retrospektivní studie MeSH
• senioři MeSH
• senzitivita a specificita MeSH
• SPECT/CT metody   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 99mTc-technecistan sodný MeSH

OBJECTIVE: To determine the diagnostic and predictive value of ultrasound and radioisotope scans of the thyroid, alone and in combination, during a single visit after initial referral by the screening laboratory with thyroid-stimulating hormone (TSH) elevation. STUDY DESIGN: Retrospective blind review of ultrasound and radioisotope images followed by final diagnosis based on clinical features, biochemistry, imaging, and molecular genetic study. RESULTS: Infants (n = 97; 61 female) with median birthweight 3.38 kg (range 2.04-4.86) and gestation 40 weeks (range 33-42), underwent successful dual thyroid ultrasound and technetium-99m pertechnetate radioisotope scan in a single center. Combined scanning at the initial visit resulted in a correct final diagnosis in 79 of 97 (81%) cases. One patient was misdiagnosed initially as having athyreosis as the result of delayed radioisotope scan and the diagnosis of ectopia made later on diagnostic challenge. The specificity/sensitivity for radioisotope scan and for ultrasound was as follows: 100%/97% and 100%/55% for ectopia (n = 39); 81%/100% and 54%/100% for athyreosis (n = 18); and 89%/90% and 80%/95% for dyshormonogenesis (n = 20). Neither modality, alone or in combination, predicted final diagnosis in eutopic glands due to hypoplasia (n = 4), transient TSH elevation (n = 12), and status still uncertain (n = 4). CONCLUSION: More than 80% of newborn infants with TSH elevation can be diagnosed correctly on initial imaging with combined radioisotope scan and ultrasound. Ultrasound cannot reliably detect thyroid ectopia. Radioisotope scan, especially if performed late, may show no uptake despite the presence of a eutopic gland.

• MeSH
• 99mTc-technecistan sodný * MeSH
• hypotyreóza krev   diagnostické zobrazování   MeSH
• konziliární vyšetření a konzultace MeSH
• lidé MeSH
• multimodální zobrazování * MeSH
• novorozenec MeSH
• novorozenecký screening metody   MeSH
• prediktivní hodnota testů MeSH
• radiofarmaka * MeSH
• radioisotopová scintigrafie MeSH
• retrospektivní studie MeSH
• thyreotropin krev   MeSH
• ultrasonografie MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 99mTc-technecistan sodný * MeSH
• radiofarmaka * MeSH
• thyreotropin MeSH

BACKGROUND: Hyaluronan (HYA) is a high molecular weight glucosaminoglycan with a great perspective for medical applications. Because HYA is widespread in the body, it is difficult to determine the fate of exogenously administered HYA. METHODS: In this study, HYAof different molecular weights (0.1-1 MDa) was labelled with (99m)Tc, and the distribution profiles were determined after administrating the HYA to rats. RESULTS: After the intravenous administration of (99m)Tc-HYA, a rapid decrease in the radioactivity of blood samples was observed, presumably because of (99m)Tc-HYA uptake by the liver; only minimal signs of liver radioactivity washout were detected. After the oral administration of (99m)Tc-HYA, no significant absorption to the central compartment was found. A preliminary study using (14)C-HYA exhibited a different distribution profile than (99m)Tc-HYA because of the different administered dose and the fate of the degradation products. Even with (14)C-HYA, only traces of radioactivity were absorbed after oral administration. CONCLUSION: This paper provides quantitative information regarding the distribution parameters of radiolabelled HYA in preclinical experiments.

• MeSH
• 99mTc-technecistan sodný MeSH
• aplikace orální MeSH
• gastrointestinální trakt metabolismus   MeSH
• gelová chromatografie MeSH
• injekce intravenózní MeSH
• játra metabolismus   MeSH
• krysa rodu Rattus MeSH
• kyselina hyaluronová krev   chemie   farmakokinetika   MeSH
• ledviny metabolismus   MeSH
• molekulová hmotnost MeSH
• orgánová specificita MeSH
• potkani Wistar MeSH
• preklinické hodnocení léčiv MeSH
• radiofarmaka MeSH
• radioizotopy uhlíku MeSH
• stabilita léku MeSH
• tkáňová distribuce MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 99mTc-technecistan sodný MeSH
• kyselina hyaluronová MeSH
• radiofarmaka MeSH
• radioizotopy uhlíku MeSH

AIM: To present our experience with the detection of bleeding in the small intestine by means of scintigraphy with in vivo-labelled red blood cells (RBCs) in the period of 1998-2009. MATERIALS AND METHODS: A 12-year prospective study was accomplished with 40 patients (23 men, 17 women, aged 12-91, mean 56 years) who had lower gastrointestinal bleeding (obscure-overt bleeding) and underwent scintigraphy with in vivo-labelled RBCs by means of technetium 99m. The scintigraphy was usually performed after other diagnostic tests had failed to locate the bleeding. RESULTS: A total of 26 patients had a positive scintigraphy with in vivo-labelled RBCs and 14 patients had negative scintigraphy. The final diagnosis was confirmed in 20 of 26 patients with a positive scintigraphy by push enteroscopy (6/20), intraoperative enteroscopy (7/20), surgery (4/20), duodenoscopy (1/20), double-balloon enteroscopy (1/20) and X-ray angiography (1/20). The correct location of the bleeding site was identified by RBC scintigraphy in 15 of 20 (75%) patients with the confirmed source. The locations of the bleeding site identified by scintigraphy and enteroscopy (push, intraoperative) and surgical investigations were highly correlated in patients with a positive scintigraphy within the first 3 h. Eleven of the 20 correctly localized studies and none of the incorrectly localized studies were positive in the dynamic phase of imaging. In 5 patients (all erroneously localized), scintigraphy was positive only at a period longer than 18 h. CONCLUSION: RBC scintigraphy is an effective imaging modality in localizing lower gastrointestinal bleeding in patients for whom other diagnostic tests have failed to locate the bleeding. RBC scintigraphy can be successful in the detection of bleeding sites in the small intestine.

• MeSH
• 99mTc-technecistan sodný * MeSH
• časové faktory MeSH
• dítě MeSH
• dospělí MeSH
• erytrocyty diagnostické zobrazování   MeSH
• gastrointestinální endoskopie MeSH
• gastrointestinální krvácení diagnostické zobrazování   MeSH
• jednofotonová emisní výpočetní tomografie * MeSH
• kolon diagnostické zobrazování   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• nemoci střev diagnostické zobrazování   MeSH
• prospektivní studie MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• tenké střevo diagnostické zobrazování   MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• Názvy látek
• 99mTc-technecistan sodný * MeSH

AIM AND BACKGROUND: We report our experience of the detection of ectopic gastric mucosa by means of perfusion Tc-99m pertechnetate disodium scintigraphy in patients with lower gastrointestinal bleeding. Gastric mucosa may be present in Meckel's diverticulum and is associated with peptic ulcer disease and lower gastrointestinal (GI) bleeding; Tc-99m pertechnetate scintigraphy can help to make the diagnosis of ectopic gastric mucosa. PATIENTS AND METHOD: Seventy-nine children (40 boys, 39 girls, aged 1 - 17, mean age 8 years) underwent Tc-99m pertechnetate scintigraphy in the last thirteen years. All patients had melena or hematochezia. Morphology imaging and endoscopy were negative in all patients. Dynamic scintigraphy of the abdomen was started immediately after i. v. administration of Tc-99m pertechnetate. The static image of the abdomen was performed subsequently. Where necessary, SPECT was additionally performed to improve the sensitivity, specificity and spatial specification. RESULTS: Three patients (two boys: two and ten years old; one girl: four years old) had positive scintigraphy results. All 3 patients underwent surgery and Meckel's diverticulum with ectopic gastric mucosa found (histology). GI bleeding stopped spontaneously in patients with a negative scintigraphy. These patients did not undergo intraoperative enteroscopy or surgery. CONCLUSION: Tc-99m pertechnetate scintigraphy can help to detect ectopic gastric mucosa in the abdomen and improve the management of patients with lower gastrointestinal bleeding.

• MeSH
• 99mTc-technecistan sodný * MeSH
• antagonisté histaminu H2 MeSH
• choristom diagnostické zobrazování   MeSH
• cimetidin MeSH
• dítě MeSH
• gastrointestinální krvácení diagnostické zobrazování   etiologie   MeSH
• kojenec MeSH
• lidé MeSH
• Meckelův divertikl komplikace   diagnóza   MeSH
• mladiství MeSH
• předškolní dítě MeSH
• radiofarmaka * MeSH
• radioisotopová scintigrafie MeSH
• ranitidin MeSH
• vylepšení obrazu MeSH
• žaludeční sliznice * MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 99mTc-technecistan sodný * MeSH
• antagonisté histaminu H2 MeSH
• cimetidin MeSH
• radiofarmaka * MeSH
• ranitidin MeSH

AIM: The article presents a case of a 24-year old man with ectopic gastric mucosa in Meckel's diverticulum. Ectopic gastric mucosa may be present in Meckel's diverticulum and may be associated with peptic ulcer disease and lower gastrointestinal (GI) bleeding. Diagnosing ectopic gastric mucosa can largely benefit from 99"Tc-pertechnetate scintigraphy. MATERIAL AND METHOD: A 24-year old man underwent Meckel's diverticulum scintigraphy for timely anamnesis of GI bleeding from uncertain source (enterorrhagia, haemorrhagic anaemia). Upper tract and large bowel endoscopy, enteroclysis, ultrasound, CT and radioactive labelled red blood cells scintigraphy resluts were negative. RESULTS: Ectopic gastric mucosa was detected by 99mTc-pertechnetate scintigraphy. The patient underwent surgery and part of the proximal ileum including Meckel's diverticulum with ectopic gastric mucosa detected by histology was resected. CONCLUSION: Meckel's diverticulum scintigraphy can help detect ectopic gastric mucosa and improve the disease management in a patient with gastrointestinal bleeding.

• MeSH
• 99mTc-technecistan sodný * MeSH
• choristom diagnostické zobrazování   MeSH
• dospělí MeSH
• gastrointestinální krvácení etiologie   MeSH
• lidé MeSH
• Meckelův divertikl komplikace   diagnostické zobrazování   patologie   MeSH
• nemoci ilea diagnostické zobrazování   MeSH
• radiofarmaka * MeSH
• radioisotopová scintigrafie MeSH
• žaludeční sliznice * MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• Názvy látek
• 99mTc-technecistan sodný * MeSH
• radiofarmaka * MeSH

Sixty-two-year-old man was admitted to hospital for increased serum level of calcium (3.85 mmol/l) and incipient renal failure (urea 7.2 mmol/l, kreatinin 117 mumol/l). The reason of hypercalcaemia was intensive explored. Serum level of intact PTH was 383.6 pg/ml. We performed two-dimensional early and delayed (2 hours) scintigram after intravenous administration of 750 MBq 99mTc-sestamibi, i.e. double-phase technique. There was detected focus (diameter 20 mm) of increased sestamibi uptake with slow wash-out in distal part right thyroid lobe. Planar scintigraphy obtained after intravenous administration of 185 MBq 99mTc-pertechnetate detected focus of increased 99mTc-pertechnetate uptake in the same point too. Patient underwent right thyroid lobectomy. There was colloid nodular goiter. Under right thyroid lobe was detected spherical particle. This particle was ablated (diameter 20 mm, weight 4 gram). It was parathyroid adenoma. One month after ablation of parathyroid adenoma serum level of PTH was 23.1 pg/ml and serum level of calcium was 2.52 mmol/l. There was overlap of scintigraphy image of parathyroid adenoma under distal part of right thyroid lobe on 99mTc-sestamibi two-dimensional scintigram and scintigraphy image of hyperplastic node with increased activity in distal part of right thyroid lobe on 99mTc-pertechnetate two-dimensional scintigram.

• MeSH
• 99mTc-technecistan sodný * MeSH
• adenom diagnostické zobrazování   MeSH
• hyperparatyreóza diagnostické zobrazování   etiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory příštítného tělíska diagnostické zobrazování   MeSH
• paratyreoidea diagnostické zobrazování   MeSH
• radiofarmaka * MeSH
• radioisotopová scintigrafie MeSH
• štítná žláza diagnostické zobrazování   MeSH
• technecium 99mTc sestamibi * MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• Názvy látek
• 99mTc-technecistan sodný * MeSH
• radiofarmaka * MeSH
• technecium 99mTc sestamibi * MeSH

The reliability of HPI measurement was studied in animal experiment. The portal vein of experimental dogs was narrowed by application of a metallic coil. The induced portal hypertension with important hepatofugal collaterals appeared to be a suitable model for the study of changes in HPI. A decrease in the index was statistically significant and corresponded to the hemodynamical efficiency of collateral circulation. The original experiment verified the reliability of HPI for an assessment of direct perfusion of the liver by portal blood.

• MeSH
• 99mTc-technecistan sodný MeSH
• jaterní oběh * MeSH
• kolaterální oběh MeSH
• portální hypertenze diagnostické zobrazování   patofyziologie   MeSH
• psi MeSH
• radioisotopová scintigrafie MeSH
• zvířata MeSH
• Check Tag
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 99mTc-technecistan sodný MeSH

• MeSH
• 99mTc-technecistan sodný MeSH
• cerebrovaskulární poruchy diagnostické zobrazování   MeSH
• dospělí MeSH
• ischemie mozku diagnostické zobrazování   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mozek diagnostické zobrazování   MeSH
• počítačová rentgenová tomografie MeSH
• radioisotopová scintigrafie MeSH
• senioři MeSH
• tranzitorní ischemická ataka diagnostické zobrazování   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 99mTc-technecistan sodný MeSH