In recent years, a dramatic increase in the prevalence of Escherichia coli strains producing extended-spectrum β-lactamases (ESBLs) has been observed - both in the community and in healthcare settings. This multicentric study aimed to characterize ESBLs produced by E. coli isolates causing hospital-onset and community urinary tract infections, as well as to compare their antimicrobial sensitivity patterns, β-lactamase content and plasmid types. Phenotypic tests for the detection of ESBLs and plasmid-mediated AmpC β-lactamases were initially pursued, followed by molecular detection of resistance genes, plasmid characterization, genotyping with pulsed-field gel electrophoresis and whole genome sequencing (WGS). The isolates exhibited high level of resistance to expanded-spectrum cephalosporins (ESC) and carried CTX-M (cefotaximase-Munich) or TEM (Temoniera) β-lactamases. All six representative isolates subjected to WGS belonged to the widespread clone ST131. In conclusion, our study demonstrated dissemination of group 1 CTX-M positive E. coli in different geographic regions of Croatia, but also different components of the health care systems (hospitals, nursing homes and the community) and confirmed the switch from SHV-2 (suphydril variant) and SHV-5 ESBLs to the nation-wide predominance of group 1 CTX-M β-lactamases. Different plasmids were shown to be associated with the dissemination of blaCTX-M genes in different geographic regions of Croatia.

• Klíčová slova
• CTX-M beta-lactamase type, E. coli, ESBL, Plasmid, Urinary tract infection,
• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence účinky léků   genetika   MeSH
• beta-laktamasy genetika   MeSH
• Escherichia coli účinky léků   genetika   izolace a purifikace   MeSH
• genetická variace MeSH
• genom bakteriální genetika   MeSH
• genotyp MeSH
• infekce močového ústrojí epidemiologie   mikrobiologie   MeSH
• infekce vyvolané Escherichia coli epidemiologie   mikrobiologie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• molekulární epidemiologie MeSH
• plazmidy genetika   MeSH
• prevalence MeSH
• retrospektivní studie MeSH
• séroskupina MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• multicentrická studie MeSH
• Geografické názvy
• Chorvatsko epidemiologie   MeSH
• Názvy látek
• antibakteriální látky MeSH
• beta-laktamasy MeSH