In thirteen different dental clinics in Singapore, Spain, Czech Republic and Italy, 504 patients were selected, and 483 dental implants were placed in maxillary sites after alveolar socket preservation (ASP) procedures with an autologous demineralized tooth extracted as graft material from an innovative Tooth Transformer device was obtained. All procedures used were reported in n°638 Ethical Committee surgical protocol of University of Chieti and approved. After 4 months, at dental implant placing, bone biopsies were performed to evaluate the histologic outcomes, and 12 months after implant loading, global implant survival rate, failure percentage and peri-implant bone loss were detected. After ASP, only 27 post-operative complications were observed and after 4 months, bone biopsy histomorphometric analysis showed a high percentage of bone volume (BV) 43.58 (±12.09), and vital new bone (NB) 32.38 (±17.15) with an absence of inflammation or necrosis areas. Twelve months after loading, only 10 dental implants failed (2.3%), with a 98.2% overall implant survival rate, nine cases showed mucositis (1.8%) and eight showed peri-implantitis (1.6%). At mesial sites, 0.43 mm (±0.83) of bone loss around the implants was detected and 0.23 mm (±0.38) at the distal sites with an average value of 0.37 mm (±0.68) (p > 0.568). Several studies with a longer follow-up will be necessary to confirm the preliminary data observed. However, clinical results seem to suggest that the post-extraction socket preservation procedure using innovative demineralized autologous tooth-derived biomaterial may be a predictable procedure to produce new vital bone able to support dental implant rehabilitation of maxilla edentulous sites.
- Klíčová slova
- alveolar socket preservation, bone grafts, dental implants, dental implants survival rate, guided bone regeneration, peri-implant bone loss,
- Publikační typ
- časopisecké články MeSH
BACKGROUND: The histological and histomorphometrical results were evaluated between vital whole and non-vital endodontically treated teeth used as autologous grafts in post-extractive socket preservation procedures. METHODS: Twenty-eight patients (average age 51.79 ± 5.97 years) with post-extractive defects were enrolled in five dentistry centers. All patients were divided into two groups: with whole teeth (Group 1) and teeth with endodontical root canal therapy (Group 2). The extracted teeth were processed with the Tooth Transformer device to obtain a demineralized and granulated graft material used with a resorbable collagen membrane for socket preservation. After four months, 32 bone biopsies were obtained for histological, histomorphometric, and statistical analysis. RESULTS: During the bone healing period, no infection signs were observed. Nineteen biopsies in group 1 and 13 biopsies in group 2 were detected. The histological analysis showed neither inflammatory nor infective reaction in both groups. Autologous grafts surrounded by new bone were observed in all samples and, at high magnification, partially resorbed dentin and enamel structures were detected. No gutta-percha or cement was identified. Small non-statistically significant differences between the groups, in total bone volume (BV), autologous graft residual, and vital bone percentage were detected. CONCLUSIONS: The study showed that the TT Transformer grafts were capable of producing new vital bone in socket preservation procedures. The histomorphometric results showed no statistical differences comparing whole and endodontically treated teeth in bone regeneration. Further studies will be carried out in order to understand the advantages of the autologous graft materials obtained from the tooth compared with the current biomaterials in bone regeneration treatments.
- Klíčová slova
- TT transformer, autologous tooth graft, bone substitute materials, bone tissue regeneration,
- Publikační typ
- časopisecké články MeSH
The aim of the study was to assess the immunohistochemical (IHC) profiles of SRC3, Pax2, ER, PgR, Her2, EGFR, CK5/6, and Ki67 proteins in breast-cancer brain metastasis. The study utilized tumor samples from 30 metastatic patients and calculated correlations between all IHC variables. In fourteen cases, primary breast cancers paired with secondary deposits were analyzed. We evaluated the association between IHC status in the primary and secondary deposits, grade, and histotype of the tumors. The examination of the metastatic deposits in all 30 patients resulted in positive detection in the following cases: SRC3 in 20 cases (66.6%), Pax2 in 22 (73.3%), ER in 22 (73.3%), PgR in 25 (83.3%), Her2 in 10 (33.3%), EGFR in 12 (40%), CK5/6 in 7 (23.3%), and Ki67 in 23 (76.6%). Grade 2 was found in 13.3% of all patients, and grade 3 in 86.7%. SRC3 and Pax2 were positive in both G2 and G3. Invasive lobular carcinoma and invasive ductal carcinoma were diagnosed in 23.3% and 76.7% of cases, respectively. There were no differences between the IHC expression of the studied proteins in either grading or histotype of the tumors. In the IHC profiles, which included SRC3, Pax2, ER, PgR, Her2, CK5/6, Ki67, and EGFR, we found no statistically significant differences between the primary cancer and the brain metastasis. In our study of metastatic breast carcinoma deposits, there was no correlation between SRC3, Pax2 status and histotype, and tumor grade. The IHC status of the paired primary and metastatic deposits did not differ in a statistically significant manner.
- MeSH
- alfa receptor estrogenů genetika metabolismus MeSH
- antigen Ki-67 genetika metabolismus MeSH
- dospělí MeSH
- erbB receptory genetika metabolismus MeSH
- karcinom diagnóza metabolismus MeSH
- koaktivátor 3 jaderných receptorů genetika metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádory mozku diagnóza metabolismus sekundární MeSH
- nádory prsu diagnóza metabolismus MeSH
- receptor erbB-2 genetika metabolismus MeSH
- receptory progesteronu genetika metabolismus MeSH
- regulace genové exprese u nádorů * MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- transkripční faktor PAX2 genetika metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alfa receptor estrogenů MeSH
- antigen Ki-67 MeSH
- erbB receptory MeSH
- ERBB2 protein, human MeSH Prohlížeč
- koaktivátor 3 jaderných receptorů MeSH
- NCOA3 protein, human MeSH Prohlížeč
- PAX2 protein, human MeSH Prohlížeč
- receptor erbB-2 MeSH
- receptory progesteronu MeSH
- transkripční faktor PAX2 MeSH
An endosseous implant is described as osseointegrated when it is immobile in function. Objective measures of stability testing have been described. The Periotest is a commercially available device that is used for this purpose. This study was designed to measure stability of endosseous implants placed in the mandible. Implants were placed in the mandibular canine or first premolar area to support an overdenture prosthesis. Stability was evaluated through the use of a Periotest device at the time of implant placement and following one year of functional loading. Implant designs were either a screw-shaped titanium alloy or a hydroxyapatite-coated cylinder. A total of 54 implants were placed, 37 were titanium screw-shaped implants, while the remaining 17 were hydroxyapatite cylinders. Initial measurements of stability showed no difference due to implant type. Following one year of functional loading, titanium screw-shaped implants were more stable than hydroxyapatite implants (P < 0.05). The difference in implant rigidity following a period of functional loading may be an indication of a difference in osseointegration between the two implants used in this study.
- MeSH
- analýza rozptylu MeSH
- biokompatibilní potahované materiály * chemie MeSH
- hydroxyapatit * chemie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mandibula chirurgie MeSH
- následné studie MeSH
- neparametrická statistika MeSH
- osteointegrace * MeSH
- parodontologie přístrojové vybavení MeSH
- pilotní projekty MeSH
- povrchové vlastnosti MeSH
- premolár MeSH
- pružnost MeSH
- retence zubní protézy přístrojové vybavení MeSH
- senioři MeSH
- slitiny MeSH
- špičák MeSH
- statistika jako téma MeSH
- titan * chemie MeSH
- zatížení muskuloskeletálního systému MeSH
- zubní implantáty * MeSH
- zubní náhrada ve spojení s implantáty MeSH
- zubní náhrady - překrytí MeSH
- zubní protéza - design * MeSH
- zubní slitiny chemie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biokompatibilní potahované materiály * MeSH
- hydroxyapatit * MeSH
- slitiny MeSH
- titan * MeSH
- titanium alloy (TiAl6V4) MeSH Prohlížeč
- zubní implantáty * MeSH
- zubní slitiny MeSH
