Human Cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) are endowed with the ability of establishing lifelong latency in human hosts and reactivating in immunocompromised subjects, including patients suffering from ulcerative colitis (UC). We, therefore, aimed to investigate virus-specific immunity in UC patients. A cohort of 24 UC patients (14 responders and 10 refractory to therapy) and 26 control subjects was prospectively enrolled to undergo virus-specific serology (by ELISA assay) and assessment of both CD4+ and CD8+ virus-specific T-cell response (by interferon-γ enzyme-linked immunospotanalysis). In parallel, mucosal viral load was determined by quantitative real-time PCR and the values were correlated with both clinical and endoscopic indexes of activity. For statistics, the t-test, Mann-Withney test, Fisher's exact test and Spearman rank correlation test were applied; p < 0.05 was considered significant. EBV-specific CD4+ and CD8+ T-cell responses were significantly lower in UC patients compared to controls (p < 0.0001 and p = 0.0006, respectively), whereas no difference was found for HCMV-specific T-cell response. When dividing the UC group according to response to therapy, both responders and refractory UC patients showed a deficient EBV-specific CD4+ T-cell response with respect to controls (p < 0.04 and p = 0.0003, respectively). Moreover, both EBV and HCMV mucosal loads were significantly higher in refractory UC than in responders and controls (p = 0.007 and 0.003; and p = 0.02 and 0.001, respectively), and correlated with activity indexes. Steroid therapy seemed the main risk factor for triggering EBV colitis. Finally, no cases of IgM positivity were found in the study population. An impaired EBV-specific immunity was clearly evident in UC patients, mostly in those refractory to therapy. The ELISPOT assay may serve as new tool for quantifying and monitoring virus-specific T-cell immunity in UC.

• Klíčová slova
• Adaptive immunity, Epstein-Barr virus, Human Cytomegalovirus, Therapy, Ulcerative colitis,
• MeSH
• CD4-pozitivní T-lymfocyty metabolismus   MeSH
• CD8-pozitivní T-lymfocyty metabolismus   MeSH
• cytomegalovirové infekce farmakoterapie   imunologie   MeSH
• Cytomegalovirus imunologie   fyziologie   MeSH
• DNA virů genetika   MeSH
• infekce virem Epsteina-Barrové farmakoterapie   imunologie   MeSH
• lidé MeSH
• prospektivní studie MeSH
• steroidy škodlivé účinky   terapeutické užití   MeSH
• studie případů a kontrol MeSH
• ulcerózní kolitida farmakoterapie   imunologie   virologie   MeSH
• virová nálož MeSH
• virus Epsteinův-Barrové imunologie   fyziologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA virů MeSH
• steroidy MeSH