MicroRNAs are emerging as important regulators of cardiac function. This study investigated the role of microRNA-24 (miR-24) in ischemic cardiomyocytes, based on the observation that miR-24 expression was significantly enhanced in the ischemic myocardium of rats. Using primary cultured rat cardiomyocytes, cell injury was induced by ischemic conditions, and the cells were evaluated for changes in lactate dehydrogenase (LDH) release, cell viability, apoptosis and necrosis. The results showed that miR-24 was increased in myocytes exposed to ischemia. When miR-24 was further overexpressed in ischemic myocytes using the mimic RNA sequence, LDH release was reduced, cell viability was enhanced, and apoptosis and necrosis rates were both decreased. By contrast, a deficiency in miR-24 resulted in the largest LDH release, lowest cell viability and highest apoptosis and necrosis rates in normal and ischemic myocytes, with significant changes compared to that of non-transfected myocytes. Additionally, the mRNA and protein levels of the pro-apoptotic gene, BCL2L11, were down-regulated by miR-24 overexpression and up-regulated by miR-24 deficiency. The luciferase reporter assay confirmed BCL2L11 to be a target of miR-24. Overall, this study showed a protective role for miR-24 against myocardial ischemia by inhibiting BCL2L11, and may represent a potential novel treatment for ischemic heart disease.
- MeSH
- apoptóza genetika MeSH
- faktor 1 indukovatelný hypoxií metabolismus MeSH
- ischemická choroba srdeční genetika metabolismus patologie MeSH
- kardiomyocyty metabolismus MeSH
- krysa rodu Rattus MeSH
- L-laktátdehydrogenasa genetika metabolismus MeSH
- membránové proteiny genetika metabolismus MeSH
- mikro RNA genetika metabolismus MeSH
- nekróza genetika MeSH
- ochranné látky metabolismus MeSH
- potkani Sprague-Dawley MeSH
- protein BCL2L11 MeSH
- proteiny regulující apoptózu genetika metabolismus MeSH
- protoonkogenní proteiny genetika metabolismus MeSH
- upregulace MeSH
- viabilita buněk genetika MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- Bcl2l11 protein, rat MeSH Prohlížeč
- faktor 1 indukovatelný hypoxií MeSH
- L-laktátdehydrogenasa MeSH
- membránové proteiny MeSH
- mikro RNA MeSH
- MIRN24 microRNA, rat MeSH Prohlížeč
- ochranné látky MeSH
- protein BCL2L11 MeSH
- proteiny regulující apoptózu MeSH
- protoonkogenní proteiny MeSH
