A yellow-pigmented bacterial strain, designated LL01(T), was isolated from hexachlorocyclohexane (HCH)-contaminated soil at Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL01(T) occupied a distinct phylogenetic position in the Sphingobium cluster, showing highest similarity to Sphingobium rhizovicinum CC-FH12-1(T) (98.5 %). The DNA G+C content of strain LL01(T) was 66.1 mol%. The predominant respiratory pigment was ubiquinone Q-10. The polar lipid profile of strain LL01(T) also corresponded to those reported for other Sphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipids), supporting its identification as a member of the genus Sphingobium. Spermidine was the major polyamine observed. The results obtained from DNA-DNA hybridization and biochemical and physiological tests clearly distinguished strain LL01(T) from closely related species of the genus Sphingobium. Therefore, strain LL01(T) represents a novel species of the genus Sphingobium, for which the name Sphingobium czechense sp. nov. is proposed (type strain LL01(T) = CCM 7979(T) = DSM 25410(T)).
- MeSH
- DNA bakterií genetika MeSH
- fylogeneze * MeSH
- hexachlorcyklohexan * MeSH
- hybridizace nukleových kyselin MeSH
- látky znečišťující půdu * MeSH
- mastné kyseliny analýza MeSH
- molekulární sekvence - údaje MeSH
- půdní mikrobiologie * MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- spermidin analýza MeSH
- Sphingomonadaceae klasifikace genetika izolace a purifikace MeSH
- techniky typizace bakterií MeSH
- ubichinon analýza MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA bakterií MeSH
- hexachlorcyklohexan * MeSH
- látky znečišťující půdu * MeSH
- mastné kyseliny MeSH
- RNA ribozomální 16S MeSH
- spermidin MeSH
- ubichinon MeSH
- Ubiquinone Q2 MeSH Prohlížeč
