Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an RNA virus responsible for coronavirus disease 2019 (COVID-19). While SARS-CoV-2 primarily targets the lungs and airways, it can also infect other organs, including the central nervous system (CNS). The aim of this study was to investigate whether the choroid plexus could serve as a potential entry site for SARS-CoV-2 into the brain. Tissue samples from 24 deceased COVID-19-positive individuals were analyzed. Reverse transcription real-time PCR (RT-qPCR) was performed on selected brain regions, including the choroid plexus, to detect SARS-CoV-2 viral RNA. Additionally, immunofluorescence staining and confocal microscopy were used to detect and localize two characteristic proteins of SARS-CoV-2: the spike protein S1 and the nucleocapsid protein. RT-qPCR analysis confirmed the presence of SARS-CoV-2 viral RNA in the choroid plexus. Immunohistochemical staining revealed viral particles localized in the epithelial cells of the choroid plexus, with the spike protein S1 detected in the late endosomes. Our findings suggest that the blood-cerebrospinal fluid (B-CSF) barrier in the choroid plexus serves as a route of entry for SARS-CoV-2 into the CNS. This study contributes to the understanding of the mechanisms underlying CNS involvement in COVID-19 and highlights the importance of further research to explore potential therapeutic strategies targeting this entry pathway.
- Klíčová slova
- COVID‐19, SARS‐CoV‐2, blood‐cerebrospinal fluid barrier, choroid plexus, neuroinvasion,
- MeSH
- COVID-19 * virologie MeSH
- dospělí MeSH
- fosfoproteiny * metabolismus MeSH
- glykoprotein S, koronavirus * genetika metabolismus MeSH
- hematoencefalická bariéra * virologie MeSH
- internalizace viru MeSH
- koronavirové nukleokapsidové proteiny MeSH
- lidé středního věku MeSH
- lidé MeSH
- plexus chorioideus * virologie MeSH
- RNA virová * genetika MeSH
- SARS-CoV-2 * fyziologie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fosfoproteiny * MeSH
- glykoprotein S, koronavirus * MeSH
- koronavirové nukleokapsidové proteiny MeSH
- nucleocapsid phosphoprotein, SARS-CoV-2 MeSH Prohlížeč
- RNA virová * MeSH
- spike protein, SARS-CoV-2 MeSH Prohlížeč
The validity of infant mortality data is essential in assessing health care quality and in the setting of preventive measures. This study explores different diagnostic procedures used to determine the cause of death across forensic settings and thus the issue of the reduced validity of data. All records from three forensic medical departments that conducted autopsies on children aged 12 months or younger (n = 204) who died during the years 2007-2016 in Moravia were included. Differences in diagnostic procedures were found to be statistically significant. Each department works with a different set of risk factors and places different emphasis on different types of examination. The most significant differences could be observed in sudden infant death syndrome and suffocation diagnosis frequency. The validity of statistical data on the causes of infant mortality is thus significantly reduced. Therefore, the possibilities of public health and social policy interventions toward preventing sudden and unexpected infant death are extraordinarily complicated by this lack of data validity.
- Klíčová slova
- SIDS, death, infant, infection, injury, sudden, suffocations, unexpected, validity of data, violent,
- Publikační typ
- časopisecké články MeSH
Breath analyzers are commonly used to test alcohol intoxication, most often to detect elevated systemic levels of ethanol by employees during working hours or drink-driving drivers. Many scientific studies describe the effect of inhaled ethanol vapors in the ambient air or the application of mouthwash before the breath test. This preliminary study interprets false positivity of the breath test after consumption over-the-counter confectionery.
- Klíčová slova
- alcohol, breath analyzer, breath-alcohol concentration, false positivity,
- MeSH
- dechové testy MeSH
- ethanol MeSH
- lidé MeSH
- otrava alkoholem * diagnóza MeSH
- pití alkoholu MeSH
- řízení motorových vozidel * MeSH
- ústní vody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ethanol MeSH
- ústní vody MeSH
The aim of this paper is to reconstruct the hijacking case of an aircraft on the Mariánské Lázně - Prague route in 1972 and the shooting of its pilot in command in light of new facts, mainly autopsy reports from both Germany and Czechoslovak Socialist Republic. These reports contained absolutely opposite findings, both in terms of the findings themselves and their resulting interpretations. Since it is still not yet known exactly what happened in the plane, a reconstruction was carried out in an identical aircraft in the Aviation Museum in Kunovice. During the reconstruction, all possible (including theoretical) situations that may have occurred in the aircraft were examined. It was found that this could not have been a premeditated intentional murder, but that the shot could only have occurred during a passenger skirmish, and one that must have resulted in a very rare situation at that. From a political-medical point of view however, there are still several questions that cannot be answered even with the best of intentions.
On 8 June 1972, the Czechoslovak OK DNN aircraft carrier L410 Turbolet was delivered to the regular line Marianske Lazne - Prague to the Federal Republic of Germany. About 8 minutes after the launch from Marianske Lazne Airport, the likely head of the group of kidnappers threw the captain of the plane. During the fight with one of the passenger, the head of the group of kidnappers killed the captain of the aircraft by a shot from 7.65 mm pistol. Other members of the hijackers group attacked other passengers. After the aircraft captains death, the second pilot took command and landed at the sports club at Weiden. After the landing, the kidnappers were detained by the police. Ten kidnappers stayed in the Federal Republic of Germany. An airplane with other passengers and coffin of a shot captain landed in Prague Ruzyne the following day. On June 12, 1972, the re-autopsy of the captain of the aircraft was performed at the Central Military Hospital in Prague. Post-autopsy status was detected. In this re-autopsy, it was possible to reconstruct the fire channel only incompletely, because during the previous autopsy performed in Federal Republic of Germany was excised the shot wound left on the neck. The fire channel began with a hole in the skin 10 cm above the right breast nipple, continued to the left and slightly upward through the subcutaneous tissue, passed through the 2nd rib at the right at a distance of 4.5 cm from the sternum, continued the lower and inner sides of the right collarbone, flowing on the front the ring cartilage and the first ring of the trachea, and on the underside of the left lobes of the thyroid gland, passed on the front of the left common carotid and continued into the area of the left SCM. The exid shot hole was on the left half of the neck. The direction of the shotway canal from right side to left side was detectable only by the fragment of the 2nd rib, which was broken out to the left and inside. The cause of the captains death was a traumatic shock. The injury was caused by a short firearm of small stance. Additional factors of firing were not found on the skin, as well as no signs of gas pressure. At the request of the experts performing the autopsy, a copy of the autopsy protocol from Germany was sent to them by an autopsied doctor. Among other things, it was reported that a 6.5 cm under the lower end of the left ear was a transversely oval, 2.5 cm long and 1.2 cm wide, red-black dried place of the upper skin, from which center run out a little red blood. At the conclusion of the autopsy protocol, it was found that there was a shotgun injury at the left side of the neck and the right side of the chest. The entrance wound according to the sent copy of the autopsy protocol, lay on the left side of the neck, the bullet penetrated the neck muscles, broke the inner branch of the cervical artery, passed through the trachea, penetrated the collarbone, split the 2nd rib to the right about 2 cm next to the sternum and the right chest 10 cm above the breast the nipple stepped out of the body. The direction of the penetration of the projectile through the body therefore had the opposite orientation compared to the re- autopsy. On the basis of this finding, the expert opinion from the re-autopsy was supplemented in the sense that there is no evidence in the autopsy protocol from Germany for the conclusion of the shot on the left side of the neck. Both autopsy experts examined the space of the aircraft cabin. The inspection showed that from the left, that is to the neck, the captain of the aircraft could be hit only if the attacker held the pistol in his left hand in a completely unnatural position so that his forearm was pulled upward over the left shoulder of the captain just to the left of the cabin and a hand bent in the wrist at a steep angle down to the right. The position of the shooters hand would be more natural if the captain of the aircraft turned backward over his left shoulder and turned the hull to the left. But this would be completely useless because of the situation, as he would look into the left cabin wall. More natural would be turning right over his right shoulder. In that case, he could be hit on the right side of the chest. The shooting kidnapper committed suicide in a cell overnight from 12 to 13 January 1973. It is not possible, based on the information available, to decide exactly how the gunshot has been going on. Czech experts did not have clothing of the captain or conclusions about their examination, and the hole on the left side of the neck was cut out by the German expert and was not provided to the Czech party through the request. It may be considered strange that the autopsy in Germany was performed in this relatively serious case outside the renowned forensic medicine department.
- Klíčová slova
- direction of shooting, exit wound, gunshot wound, input wound, kidnapping of an aircraft,
- MeSH
- letadla MeSH
- lidé MeSH
- pitva MeSH
- sebevražda * MeSH
- soudní lékařství MeSH
- střelné rány * MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Serious intrapartum fetal injuries are unfortunate events that confer severe consequences on medical personnel. Most birth traumas are noncritical and resolve for a few days. Permanent effects or fatal outcomes occur infrequently. We report an unusual case of intrapartum complete fetal decapitation. The labor was complicated by shoulder dystocia, with resultant repeated mechanical trauma to the fetal neck and, finally, decapitation. The tragic results of biological processes in human organisms do not automatically confirm medical malpractice. However, there may be grave ethical and forensic outcomes.
- MeSH
- dekapitace * MeSH
- dospělí MeSH
- dystokie ramének * MeSH
- indukovaný porod MeSH
- lidé MeSH
- novorozenec MeSH
- porodní poranění novorozence komplikace MeSH
- těhotenství MeSH
- vakuová extrakce porodnická škodlivé účinky MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- novorozenec MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
PURPOSE: To analyze the reasons and patient-related and injury-related risk factors for reoperation after surgery for acute subdural hematoma (SDH) and the effects of reoperation on treatment outcome. METHODS: Among adult patients operated on for acute SDH between 2013 and 2017, patients reoperated within 14 days after the primary surgery were identified. In all patients, parameters were identified that related to the patient (age, anticoagulation, antiplatelet, and antiepileptic treatment, and alcohol intoxication), trauma (Glasgow Coma Score, SDH thickness, midline shift, midline shift /hematoma thickness rate, other surgical lesion, primary surgery-trephination, craniotomy, or decompressive craniotomy), and Glasgow Outcome Score (GOS). The reasons for reoperation and intervals between primary surgery and reoperation were studied. RESULTS: Of 86 investigated patients, 24 patients were reoperated (27.9%), with a median interval of 2 days between primary surgery and reoperation. No significant differences in patients and injury-related factors were found between reoperated and non-reoperated patients. The rate of primary craniectomies was higher in non-reoperated patients (P = 0.066). The main indications for reoperation were recurrent /significant residual SDH (10 patients), contralateral SDH (5 patients), and expansive intracerebral hematoma or contusion (5 patients). The final median GOS was 3 in non-reoperated and 1.5 in reoperated patients, with good outcomes in 41.2% of non-reoperated and 16.7% of reoperated patients. CONCLUSIONS: Reoperation after acute SDH surgery is associated with a significantly worse prognosis. Recurrent /significant residual SDH and contralateral SDH are the most frequently found reasons for reoperation. None of the analyzed parameters were significant reoperation predictors.
- Klíčová slova
- Acute subdural hematoma, Brain injury, Contralateral subdural hematoma, Decompressive craniectomy, Reoperation,
- MeSH
- akutní subdurální hematom epidemiologie etiologie chirurgie MeSH
- antikoagulancia terapeutické užití MeSH
- antikonvulziva terapeutické užití MeSH
- dekompresní kraniektomie statistika a číselné údaje MeSH
- dospělí MeSH
- epilepsie farmakoterapie epidemiologie MeSH
- Glasgowská stupnice kómat MeSH
- Glasgowská stupnice následků MeSH
- inhibitory agregace trombocytů terapeutické užití MeSH
- kraniocerebrální traumata komplikace MeSH
- kraniotomie statistika a číselné údaje MeSH
- lidé středního věku MeSH
- lidé MeSH
- neurochirurgické výkony statistika a číselné údaje MeSH
- otrava alkoholem epidemiologie MeSH
- reoperace statistika a číselné údaje MeSH
- rizikové faktory MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- sexuální faktory MeSH
- trepanace statistika a číselné údaje MeSH
- věkové faktory MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antikoagulancia MeSH
- antikonvulziva MeSH
- inhibitory agregace trombocytů MeSH
Postmortem facial identification is one of the most common techniques for establishing a deceased person's identity. In victims suffering from devastating cranial injuries, the feasibility of facial identification tasks can be compromised by damage to or disfigurement of the identifying cranial features. Although there are several reconstructive approaches, which help experts to restore the essence of person's physical appearance, thus enhancing the chances of recognition, only a few of them involve restoring the fractured cranial bones as the foundation for the reconstructed soft tissues. Here, we propose a technique based on replacement of heavily damaged hard tissues with generic prosthetics manufactured by 3D printing. Our approach does not require medical imaging technologies or other costly lab equipment. It is simple, affordable and relatively labor-efficient. The deceased's reconstructed craniofacial features can be subsequently assessed, photographed, drawn or otherwise reproduced in order to help determine his or her identity. In addition, the imagery can be displayed, published or broadcasted in media without concerns of being overly graphic.
- Klíčová slova
- 3D printing, Autopsy, Facial identification, Postmortem examination, Reconstructive techniques,
- Publikační typ
- časopisecké články MeSH
Recent advances in unmanned aerial technology have substantially lowered the cost associated with aerial imagery. As a result, forensic practitioners are today presented with easy low-cost access to aerial photographs at remote locations. The present paper aims to explore boundaries in which the low-end drone technology can operate as professional crime scene equipment, and to test the prospects of aerial 3D modeling in the forensic context. The study was based on recent forensic cases of falls from height admitted for postmortem examinations. Three mock outdoor forensic scenes featuring a dummy, skeletal remains and artificial blood were constructed at an abandoned quarry and subsequently documented using a commercial DJI Phantom 2 drone equipped with a GoPro HERO 4 digital camera. In two of the experiments, the purpose was to conduct aerial and ground-view photography and to process the acquired images with a photogrammetry protocol (using Agisoft PhotoScan® 1.2.6) in order to generate 3D textured models. The third experiment tested the employment of drone-based video recordings in mapping scattered body parts. The results show that drone-based aerial photography is capable of producing high-quality images, which are appropriate for building accurate large-scale 3D models of a forensic scene. If, however, high-resolution top-down three-dimensional scene documentation featuring details on a corpse or other physical evidence is required, we recommend building a multi-resolution model by processing aerial and ground-view imagery separately. The video survey showed that using an overview recording for seeking out scattered body parts was efficient. In contrast, the less easy-to-spot evidence, such as bloodstains, was detected only after having been marked properly with crime scene equipment.
- Klíčová slova
- 3D scene mapping, Aerial photography, Drones, Forensic scene documentation, Photogrammetry, Real-time survey,
- MeSH
- audiovizuální záznam přístrojové vybavení MeSH
- fotografování přístrojové vybavení MeSH
- fotogrammetrie MeSH
- krevní skvrny MeSH
- letadla * MeSH
- lidé MeSH
- počítačová simulace MeSH
- soudní vědy přístrojové vybavení MeSH
- technologie dálkového snímání * MeSH
- tělesné pozůstatky MeSH
- zobrazování trojrozměrné * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Inhabitants of the Indonesian island of Sumatra are faced with the problem of insufficient food supplies and the consequent risk of undernourishment and health issues. Edible insects as a traditional and readily available food source could be part of the solution. The nutritional value of insects depends on many factors, e.g., species, developmental stage, sex, diet, and climatic conditions. However, edible insects bred in Sumatra for human consumption have never before been assessed with regard to their nutritional value. Our study involved analyses of crude protein, chitin, fat and selected fatty acid contents of giant mealworm larvae (Zophobas morio), larvae of the common mealworm (Tenebrio molitor) and nymphs of the field cricket (Gryllus assimilis). Crude protein content in the samples ranged from 46% to 56%. Highest (35%) and lowest (31%) amounts of fat were recorded in giant mealworm larvae and larvae of the common mealworm, respectively. Chitin amounts ranged from 6% to 13%. Based on these values, which are comparable to those known from other food insects reared in different regions of the world, the edible species bred in Sumatra could become food sources with a potential to help stave off hunger and undernourishment.
- Klíčová slova
- Gryllus assimilis, Indonesia, Tenebrio molitor, Zophobas morio, amino acid profile, chitin, crude protein, edible insect, fats,
- MeSH
- brouci chemie růst a vývoj MeSH
- Gryllidae chemie růst a vývoj MeSH
- larva chemie růst a vývoj MeSH
- lidé MeSH
- nutriční hodnota * MeSH
- nymfa chemie růst a vývoj MeSH
- Tenebrio chemie růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indonésie MeSH
