Oceanic harmful algal blooms of Pseudo-nitzschia diatoms produce the potent mammalian neurotoxin domoic acid (DA). Despite decades of research, the molecular basis for its biosynthesis is not known. By using growth conditions known to induce DA production in Pseudo-nitzschia multiseries, we implemented transcriptome sequencing in order to identify DA biosynthesis genes that colocalize in a genomic four-gene cluster. We biochemically investigated the recombinant DA biosynthetic enzymes and linked their mechanisms to the construction of DA's diagnostic pyrrolidine skeleton, establishing a model for DA biosynthesis. Knowledge of the genetic basis for toxin production provides an orthogonal approach to bloom monitoring and enables study of environmental factors that drive oceanic DA production.

• MeSH
• eutrofizace * MeSH
• kyselina kainová analogy a deriváty   chemie   metabolismus   MeSH
• multigenová rodina MeSH
• neurotoxiny biosyntéza   genetika   MeSH
• rekombinantní proteiny genetika   metabolismus   MeSH
• rozsivky genetika   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• domoic acid MeSH Prohlížeč
• kyselina kainová MeSH
• neurotoxiny MeSH
• rekombinantní proteiny MeSH