- Autor
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Pracoviště
Center for Marine Biotechnology and B... 1 Department of Biology Dalhousie Unive... 1 Institute of Parasitology University ... 1 Integrative Oceanography Division Scr... 1 Marine and Environmental Biology Depa... 1 Microbial and Environmental Genomics ... 1 Moss Landing Marine Laboratories 8272... 1 Skaggs School of Pharmacy and Pharmac... 1 Synthetic Biology and Bioenergy Group... 1
- Publikační typ
- Kategorie
- Jazyk
- Země
- Časopis/zdroj
- Země - grant
- Grantová agentura
- Nejvíce citované
- Autor
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Pracoviště
Center for Marine Biotechnology and B... 1 Department of Biology Dalhousie Unive... 1 Institute of Parasitology University ... 1 Integrative Oceanography Division Scr... 1 Marine and Environmental Biology Depa... 1 Microbial and Environmental Genomics ... 1 Moss Landing Marine Laboratories 8272... 1 Skaggs School of Pharmacy and Pharmac... 1 Synthetic Biology and Bioenergy Group... 1
- Publikační typ
- Kategorie
- Jazyk
- Země
- Časopis/zdroj
- Země - grant
- Grantová agentura
- Nejvíce citované
PubMed
30262498
PubMed Central
PMC6276376
DOI
10.1126/science.aau0382
PII: 361/6409/1356
Knihovny.cz E-zdroje
Oceanic harmful algal blooms of Pseudo-nitzschia diatoms produce the potent mammalian neurotoxin domoic acid (DA). Despite decades of research, the molecular basis for its biosynthesis is not known. By using growth conditions known to induce DA production in Pseudo-nitzschia multiseries, we implemented transcriptome sequencing in order to identify DA biosynthesis genes that colocalize in a genomic four-gene cluster. We biochemically investigated the recombinant DA biosynthetic enzymes and linked their mechanisms to the construction of DA's diagnostic pyrrolidine skeleton, establishing a model for DA biosynthesis. Knowledge of the genetic basis for toxin production provides an orthogonal approach to bloom monitoring and enables study of environmental factors that drive oceanic DA production.
- MeSH
- eutrofizace * MeSH
- kyselina kainová analogy a deriváty chemie metabolismus MeSH
- multigenová rodina MeSH
- neurotoxiny biosyntéza genetika MeSH
- rekombinantní proteiny genetika metabolismus MeSH
- rozsivky genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- domoic acid MeSH Prohlížeč
- kyselina kainová MeSH
- neurotoxiny MeSH
- rekombinantní proteiny MeSH
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