This paper provides the first detailed description of a Tetracapsuloides species, Tetracapsuloides vermiformis n. sp., with vermiform stages in the bryozoan host, Fredericella sultana, and its experimental transmission from F. sultana to Cyprinus carpio. The suitability of morphological, biological and 18S rDNA sequence data for discrimination between malacosporean species is reviewed and recommendations are given for future descriptions. Presently, malacosporean species cannot be differentiated morphologically due to their cryptic nature and the lack of differential characters of spores and spore-forming stages in both hosts. We examined biological, morphological and molecular characters for the present description and for revising malacosporean taxonomy in general. As a result, Buddenbrockia plumatellae was split into two species, with its sac-like stages being ascribed to Buddenbrockia bryozoides n. comb. In addition to ribosomal DNA sequences multiple biological features rather than morphological characters are considered essential tools to improve malacosporean taxonomy in the future according to our analysis of the limited traits presently available.

• Klíčová slova
• Buddenbrockia bryozoides n. comb., Buddenbrockia plumatellae, Tetracapsuloides vermiformis n, Malacosporea, Morphological variation, Myxozoa, Species discrimination, Taxonomy, sp,
• MeSH
• Bryozoa parazitologie   MeSH
• interakce hostitele a parazita MeSH
• kapři MeSH
• Myxozoa genetika   fyziologie   MeSH
• nemoci ryb parazitologie   MeSH
• organismy bez specifických patogenů MeSH
• parazitární nemoci u zvířat parazitologie   přenos   MeSH
• RNA ribozomální 18S genetika   MeSH
• stadia vývoje MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• RNA ribozomální 18S MeSH

Monoclonal antibody B4 (mAb B4) was previously developed to the myxozoan parasite Tetracapsuloides bryosalmonae Canning, Curry, Feist, Longshaw et Okamura, 1999, the causative agent of proliferative kidney disease of salmonids, Here we describe the reaction of mAb B4 against Myxobolus cerebralis Hofer, 1903, the parasite that causes 'whirling disease' in salmonids. Tissues examined were collected from experimentally infected rainbow trout Oncorhynchus mykiss (Walbaum) and the aquatic oligochaete Tubifex tubifex (O.F. Müller), the two hosts involved in the life cycle of M. cerebralis. Paraffin sections of infected rainbow trout taken at 4 h and 3, 10, 17 and 54 days post-exposure to infective M. cerebralis actinospores were immunohistochemically stained with mAb B4. Longitudinal sections through infected T. tubifex sampled 120 days post-exposure to M. cerebralis myxospores were also examined using this method. The only phase of the M. cerebralis life cycle that expressed the mAb B4 antigen was during sporogenesis in the salmonid host. The immunohistochemical staining demonstrated that the antigen was released into the tissues surrounding the spore and sporogonic stages of the parasite. The localisation of the antigen was diffuse in the fish, suggesting that the possible effect of M. cerebralis infection is extensive through the head tissues and not limited to areas of cartilage destruction as previously thought.

• MeSH
• antigeny protozoální imunologie   MeSH
• Eukaryota imunologie   fyziologie   MeSH
• imunohistochemie veterinární   MeSH
• monoklonální protilátky imunologie   MeSH
• nemoci ryb imunologie   parazitologie   patologie   MeSH
• Oligochaeta cytologie   parazitologie   MeSH
• Oncorhynchus mykiss MeSH
• protozoální infekce zvířat imunologie   patologie   MeSH
• stadia vývoje fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny protozoální MeSH
• monoklonální protilátky MeSH