Cytokinins are adenine and non-adenine derived heterogeneous class of regulatory molecules that participate in almost every aspect of plant biology. They also affect plant defense responses as well as help microbial pathogens to establish pathogenesis. The functional approaches that ensure desired and subtle modulations in the levels of plant cytokinins are highly instrumental in assessing their functions in plant immunity. Here, we describe a detailed working protocol regarding the enhanced production of cytokinins from plants that harbor isopentenyltransferase (IPT) enzyme gene under the control of 4xJERE (jasmonic acid and elicitor-responsive element) pathogen-inducible promoter. Our devised expression system is a context-dependent solution when it comes to investigating host-pathogen interactions under the modulated conditions of plant cytokinins.
- Klíčová slova
- Cytokinins, Host-pathogen interaction, Pathogen-inducible promoter, Transient plant transformation,
- MeSH
- cytokininy metabolismus MeSH
- fenotyp MeSH
- geneticky modifikované rostliny MeSH
- imunita rostlin MeSH
- interakce hostitele a patogenu * imunologie MeSH
- nemoci rostlin genetika imunologie mikrobiologie MeSH
- regulace genové exprese u rostlin MeSH
- regulátory růstu rostlin metabolismus MeSH
- signální transdukce MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cytokininy MeSH
- regulátory růstu rostlin MeSH
