UNLABELLED: Primary graft failure occurs 15 to 30 % of the time after transplantation. Although there have been improvements in preserving the lungs in good condition, there have not been studies on the regulation of transcription factors. METHODS: We carried out an experimental study involving lung transplantation to indirectly evaluate reactive oxygen species (ROS) production and VEGF expression by competitive blockade of HIF-1alpha with chetomin. There were 5 groups: Group-1: Lung blocks were perfused with 0.9 % SSF, immediately harvested, and preserved. Group-2 (I-T): Immediate transplantation and then reperfusion for 1 h. Group-3 (I-R): Lung blocks were harvested and preserved in LPD solution for 6 h and reperfused for 1 h. Group-4 (DMSO): Lung blocks were treated for 4 h with DMSO, preserved for 6 h and transplanted to a receptor treated with DMSO. Group-5 (chetomin): Lung blocks were treated for 4 h with chetomin, preserved for 6 h and transplanted to a receptor treated with chetomin. ROS, mRNA, and protein levels of HIF-1alpha and EG-VEGF were determined. RESULTS: The DMSO and chetomin groups had significantly lower ROS levels. Compared with those in the I-R group, the chetomin group exhibited the lowest level of HIF-1alpha. CONCLUSIONS: Addition of chetomin to the donor and the receptor results in a significant reduction in HIF-1A, VEGF and ROS.
- MeSH
- disulfidy MeSH
- faktor 1 indukovatelný hypoxií - podjednotka alfa * metabolismus MeSH
- indolové alkaloidy MeSH
- krysa rodu Rattus MeSH
- plíce metabolismus účinky léků MeSH
- potkani Sprague-Dawley MeSH
- reaktivní formy kyslíku * metabolismus MeSH
- transplantace plic * MeSH
- vaskulární endoteliální růstový faktor A * metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chetomin MeSH Prohlížeč
- disulfidy MeSH
- faktor 1 indukovatelný hypoxií - podjednotka alfa * MeSH
- Hif1a protein, rat MeSH Prohlížeč
- indolové alkaloidy MeSH
- reaktivní formy kyslíku * MeSH
- vascular endothelial growth factor A, rat MeSH Prohlížeč
- vaskulární endoteliální růstový faktor A * MeSH