Magnetic harvesting of microalgal biomass provides an attractive alternative to conventional methods. The approach to this issue has so far been pragmatic, focused mainly on finding cheap magnetic agents in combination with harvestable microalgae species. The aim of this work was to study experimentally and theoretically the mechanisms leading to cell-magnetic agent attachment/detachment using real experiments and predictions made by colloidal adhesion (XDLVO) model. Two types of well defined magnetic beads (MBs) carrying ion exchange functional groups (DEAE - diethylaminoethyl and PEI - polyethylenimine) were studied in connection with microalgae (Chlorella vulgaris). Optimal harvesting efficiencies (>90%) were found for DEAE and PEI MBs, while efficient detachment was achieved only for DEAE MBs (>90%). These findings were in accordance with the predictions by XDLVO model. Simultaneously there was found a discrepancy between the XDLVO prediction and the poor detachment of PEI MBs from microalgal surface. This can be ascribed to an additional interaction (probably covalent bonds) between PEI and algal surface, which the XDLVO model is unable to capture given by its non-covalent nature.
- Klíčová slova
- Cell adhesion, Magnetic beads, Microalgae, Surface interactions, XDLVO theory,
- MeSH
- biologické modely MeSH
- biomasa MeSH
- buněčná adheze MeSH
- Chlorella vulgaris izolace a purifikace fyziologie MeSH
- ethanolaminy chemie MeSH
- iontová výměna MeSH
- koloidy MeSH
- magnetické jevy MeSH
- magnetické nanočástice chemie MeSH
- mikrořasy izolace a purifikace fyziologie MeSH
- polyethylenimin chemie MeSH
- povrchové vlastnosti MeSH
- průmyslová mikrobiologie metody MeSH
- sladká voda mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 2-diethylaminoethanol MeSH Prohlížeč
- ethanolaminy MeSH
- koloidy MeSH
- magnetické nanočástice MeSH
- polyethylenimin MeSH
