In our previous studies, a decreased blood pressure was reported in children treated by anthracycline (AC). The aim of this study was to assess the long-term effects of AC anticancer therapy in 45 subjects aged 13-22 years by repeated 24-hour Holter monitoring of blood pressure. Sixty four aged-matched subjects served as controls. The differences between mean values of systolic (SBP) and diastolic blood pressure (DBP) in each hour of both groups were evaluated by Mann-Whitney test. Also the parameters of the least-squares fit of the sinusoidal curve in each subject were estimated (M - mesor, midline-estimating, a mean value of sinusoidal curve corresponds to 24-hours mean pressure; A - amplitude, double amplitude corresponds to night-day difference; Acr - acrophase is a time of maximal value of a sinusoidal curve). SBP and DBP was significantly lower only during night hours in anthracycline patients 19-22 years old. Also M was lower in this age subgroup of patients comparing to age matched controls (SBP: 112+/-6 mm Hg versus 117+/-7 mm Hg, p<0.05; DBP: 67+/-3 mm Hg versus 69+/-6 mm Hg, p<0.05), A was not different, Acr in patients was shifted one hour earlier (SBP: 2.4 p.m. versus 3.6 p.m., p<0.05; DBP: 2.1 p.m. versus 3.3 p.m., p<0.01). This corresponds to the shift of the morning blood-pressure increase seen on 24-hours blood pressure profiles. M correlated with age in controls (SBP: r=0.374, p<0.01; regression coefficient b=1.34 mm Hg/1 year; DBP: r=0.365, p<0.01; b=0.95 mm Hg/1 year), but not in patients (SBP: r=0.182, DBP: r=0.064). A and Acr were age-independent in all subjects. It is concluded that blood pressure in 19-22 years old AC patients is lower during night hours, the age-dependent increase of blood pressure seen in healthy controls between 13 and 22 years of age does not occur in patients. This finding is consistent with the long-lasting impairment of the sympathetic nervous system caused by anthracyclines.
- MeSH
- antibiotika antitumorózní škodlivé účinky MeSH
- antracykliny škodlivé účinky MeSH
- cirkadiánní rytmus * MeSH
- elektrokardiografie ambulantní MeSH
- krevní tlak účinky léků MeSH
- lidé MeSH
- metoda nejmenších čtverců MeSH
- mladiství MeSH
- mladý dospělý MeSH
- přežívající * MeSH
- studie případů a kontrol MeSH
- sympatický nervový systém účinky léků patofyziologie MeSH
- věkové faktory MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibiotika antitumorózní MeSH
- antracykliny MeSH
UNLABELLED: The analysis of short-term blood pressure regulation in children, adolescents and young adults 1 to 18 years after the treatment with anthracyclines known to have cardiotoxic side effects for oncological diseases was the aim of the present study. Thirty-one subjects treated with anthracyclines (PA) and 11 subjects treated with different antitumour drugs (P0) were investigated twice (the interval between two investigations 1-9 years). Three hundred and thirty-nine healthy subjects served as controls (C). Systolic (SBP), diastolic blood pressures (DBP) in the finger arteries and inter-beat interval (IBI) were recorded beat-to-beat (FINAPRES, Ohmeda, metronome controlled breathing, 5 minute recording); the values were corrected by auscultatory blood pressure measurements. Baroreflex sensitivity (BRS, ms/mmHg) was determined by a spectral method. As the investigated subjects were of different ages, the measured values were standardised on the age of 16 years by linear regression, and only standardised values (IBI16, SBP16, DBP16 and BRS16) were further analysed. No differences were found between PA, P0 and C in BRS16 and IBI16. SBP16 and DBP16 were significantly lower in PA (102.1+/-8.3/59.7+/-7.1 versus C: 114.1+/-12.4/69.0+/-9.5 mmHg; p<0.001/p<0.001; mean from two investigations). SBP16 but not DBP16 was also lower in P0 (102.7+/-12.6/64.5+/-9.7 mmHg; p<0.01/no significant) than in C. The correlation coefficient between SBP16 and period after treatment in PA was -0.11 (no significant) and -0.06 in DBP16 (no significant). Thus, there is not seen a trend to normalisation. CONCLUSION: The anthracycline antitumour therapy in children decreases blood pressure and within 18 years after the treatment there is not observed a trend toward normal values. BRS was not influenced by the anthracycline therapy.
- MeSH
- akutní lymfatická leukemie farmakoterapie metabolismus patologie MeSH
- antracykliny terapeutické užití MeSH
- arterie účinky léků patologie MeSH
- baroreflex fyziologie MeSH
- dítě MeSH
- Hodgkinova nemoc farmakoterapie metabolismus patologie MeSH
- index tělesné hmotnosti MeSH
- kojenec MeSH
- krevní tlak fyziologie MeSH
- lidé MeSH
- mladiství MeSH
- monitory krevního tlaku MeSH
- nehodgkinský lymfom farmakoterapie metabolismus patologie MeSH
- předškolní dítě MeSH
- srdeční frekvence MeSH
- studie případů a kontrol MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- randomizované kontrolované studie MeSH
- srovnávací studie MeSH
- Názvy látek
- antracykliny MeSH
Thermosensitive submerged endospores formed by Streptomyces globisporus 0234 and its natural variant A resembled those of thermoresistant actinomycetes not only in their morphology ultrastructure, but also in the content of dipicolinic acid. The production of endospores containing this substance is unusual in Streptomyces while other features of the strain indicate relatedness to other streptomycetes. Chemotaxonomic analysis of variant A revealed the cell wall to be of chemotype I and fatty acid content typical of Streptomyces. Most characteristics of surface cultures of variant A coincided with those of the original strain 0234 and its endosporeless variant B. Both the strain 0234 and its variants A and B produced identical antibiotics and pesticidal compounds.
An asporogenous spontaneous mutant of Streptomyces aureofaciens named ASR1 was selected on streptomycin gradient plates. The mutant is very stable and differs in ultrastructure and morphology, it is prototrophic but it lost the ability to grow well on soybean extract medium and produces one-tenth tetracyclines of the parent. The ASR1 mutant has a 3-4-fold increased resistance to streptomycin and is cross-resistant to other aminoglycosides. Comparison of the protein profiles from both strains on SDS gels revealed a very low expression of a 29.5 kDa protein in the ASR1 mutant which is overexpressed in both vegetative cells and spores of the parental strain.
- MeSH
- aminoglykosidy MeSH
- antibakteriální látky farmakologie MeSH
- antibiotická rezistence genetika MeSH
- bakteriální proteiny genetika izolace a purifikace MeSH
- mutace MeSH
- spory bakteriální genetika MeSH
- Streptomyces aureofaciens účinky léků genetika ultrastruktura MeSH
- tetracyklin biosyntéza MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aminoglykosidy MeSH
- antibakteriální látky MeSH
- bakteriální proteiny MeSH
- tetracyklin MeSH
Ultrahistochemical method according to Thiéry (1967) was used to determine the occurrence and localization of glycogen in blastocysts developed in vivo and in blastocysts developed from 2-cell embryos of the mouse for 62 to 64 h in in vitro culture. The presence of glycogen was found in blastocysts of both experimental groups. Glycogen had a monoparticulate character, i.e. the form of beta-granules, localized above all in the ground cytoplasm of cells. Their size varied from 10 to 30 nm. In the blastocysts developed in the physiological uterine environment the glycogen content was relatively low, trophoblasts cells containing regularly a higher amount of glycogen particles than embryoblast cells. In the blastocysts developed in the culture medium in the presence of currently used energy sources the distribution and content of glycogen were clearly graded according to the cell types. Compared with the in vivo-blastocysts, an abnormally high amount of glycogen was observed in the cytoplasm of trophoblast cells, a medium amount in the prospective endoderm cells and the minimum amount in the prospective ectoderm cells. The authors are of the opinion that differences in the accumulation of glycogen and its occurrence in the individual cells are in connection with their position in the blastocyst and with their relation to the surrounding microenvironment. It can be judged from the findings of glycogen deposits inside autophagic vacuoles and multivesicular bodies as well as inside extracellular located sacs that simultaneously with glycogen accumulation there also proceeds its partial degradation in lysosomal structures of blastocyst cells.
- MeSH
- blastocysta fyziologie ultrastruktura MeSH
- buněčné jádro ultrastruktura MeSH
- elektronová mikroskopie MeSH
- glykogen analýza MeSH
- myši MeSH
- vakuoly ultrastruktura MeSH
- zona pellucida ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- glykogen MeSH
DNA-directed RNA polymerase was isolated from wild-type and mutant (asporogenic and granaticin overproducing) Streptomyces granaticolor. The effect of granaticin on the enzyme activity was compared with that of standard transcription inhibitors, rifampicin and rifamycin SV and shown to be zero.
- MeSH
- antibakteriální látky farmakologie MeSH
- DNA řízené RNA-polymerasy metabolismus MeSH
- naftochinony farmakologie MeSH
- Streptomyces účinky léků enzymologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- DNA řízené RNA-polymerasy MeSH
- granaticin MeSH Prohlížeč
- naftochinony MeSH
This study is based on the analysis of nation-wide notification data on the bacteriologically proved mycobacterial infections registered in the national Information System on Bacillary Tuberculosis. Over the 5-year period under study, as many as 2,226 M. tuberculosis isolations were on average reported annually in the country (i.e. 21.6 per 100,000 population), and of these 62.5% were isolations in males, 63.5% were repeated isolations and 87% were isolations from sputum specimens; identification of human of M. bovis infection was reported in 13 patients annually only, mostly in those of higher age categories. The annual average of notified M. kansasii isolations was 138 (1.3 per 100,000 population), repeated isolations of the agent were reported in 66.6% of patients, mostly males 45-54 years old, and 81% of all reports came from an endemic area in the North-Moravian Region; notification of positive M. avium-intracellulare isolations occurred in 24 persons annually, in 48% of these repeatedly, M. xenopi isolation was reported in 87 and M. fortuitum/chelonei in 12 cases annually.
- MeSH
- dítě MeSH
- dospělí MeSH
- epidemiologické metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- Mycobacterium tuberculosis izolace a purifikace MeSH
- Mycobacterium izolace a purifikace MeSH
- mykobakteriózy epidemiologie mikrobiologie MeSH
- předškolní dítě MeSH
- senioři MeSH
- sexuální faktory MeSH
- sputum mikrobiologie MeSH
- tuberkulóza epidemiologie mikrobiologie MeSH
- věkové faktory MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
Intradermal skin tests with a 2TU dose of PPD-RT 23 prepared from M. tuberculosis and 0.1 ug/0.1 ml of PPD-RS 631 from M. xenopi were simultaneously carried out in 378 7-year-old children from two localities in North-Bohemian region's capital Ustí n. Lab., a focus of M. xenopi endemicity repeatedly confirmed since its disclosure in 1980 by positive M. xenopi isolations from humans and public water supply network. A further group 157 children serving as controls was from Prague district 4 where no presence of M. xenopi strains was ever recorded. All of these children had received routine immunization at birth with Czech BCG vaccine. The children from the two endemic localities were found to give a positive 6 mm or greater reaction to M. xenopi mycobacterin in 43.3% and 22.3%, to human tuberculin in 12.8% and 12.6%, respectively. The frequency histogram clearly separated a group of reactors with 8-18 mm indurations from a group of nonreactors showing a skin induration of 4-8 mm. The higher reactivity of this exposed child population was also reflected in a larger proportion of reactions greater to M. xenopi PPD than to human tuberculin antigen: the reactions greater by 1-5 mm accounted, respectively, for 25.1% and 20.6%, reactions greater by 6 mm or more for 23.7% and 15.9%. Among a group of children from Prague district 4, 6.4% had medium-sized and 3.8% large-sized reactions to M. xenopi antigen; the proportion of reactions greater to M. xenopi antigen than to human tuberculin accounted for only 5.1%, reactions greater to tuberculin than to sensitin were here in slight predominance. The evidenced skin sensitization to M. xenopi mycobacterin is suggested to result from the different degrees of exposure to infection by environmental mycobacteria.
- MeSH
- antigeny bakteriální imunologie MeSH
- atypické mykobakteriální infekce epidemiologie MeSH
- dítě MeSH
- intradermální testy * MeSH
- kožní testy * MeSH
- lidé MeSH
- mikrobiologie vody MeSH
- Mycobacterium imunologie MeSH
- mykobakteriózy epidemiologie MeSH
- netuberkulózní mykobakterie imunologie MeSH
- tuberkulinový test MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Československo MeSH
- Názvy látek
- antigeny bakteriální MeSH
A thy- tetracycline-resistant mutant of Streptomyces granaticolor was prepared by mutagenesis of the parental strain ETH 7437. The mutant exhibits a different morphology and an overproduction of granaticins. The ability to form an aerial mycelium and spores has been lost. The mutant cells have a round or an atypical shape and a thick cell wall, the membraneous system is enlarged by numerous mesosomes. Division septa are formed rarely. The mutant is more sensitive to both low and high temperature than the parental strain. The altered features are stably maintained for many generations. Ribosomal proteins of the mutant do not differ substantially from those of the original strain indicating that the mutant phenotype is not due to an alteration at the translational level.
- MeSH
- antibiotika antitumorózní metabolismus MeSH
- chromatografie na tenké vrstvě MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- elektronová mikroskopie MeSH
- fermentace MeSH
- mutace MeSH
- naftochinony metabolismus MeSH
- rezistence na tetracyklin MeSH
- ribozomální proteiny analýza MeSH
- Streptomyces genetika růst a vývoj metabolismus ultrastruktura MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibiotika antitumorózní MeSH
- granaticin MeSH Prohlížeč
- naftochinony MeSH
- ribozomální proteiny MeSH
The growth of Alcaligenes eutrophus in the presence of benzylpenicillin under heterotrophic and autotrophic conditions was studied. The drug induced a penicillinase in the cells, which can be readily released and extracted from the cells after a lysozyme and EDTA treatment in the course of spheroplast formation. The isoelectric point of the enzyme is 8.1 and the molar mass was estimated to be nearly 25 kg/mol. Phenoxypenicillin is hydrolyzed in the presence of the enzyme at a higher relative rate than benzylpenicillin, ampicillin, amoxycillin and azlocillin. The cephalosporins tested, i.e. cephalosporin C, cefalexin, cefotaxime and 7-aminocephalosporanic acid, were hydrolyzed at a substantially lower relative rate than the penicillins, indicating that the enzyme is a penicillinase.
- MeSH
- Alcaligenes enzymologie MeSH
- enzymová indukce MeSH
- isoelektrická fokusace MeSH
- kinetika MeSH
- molekulová hmotnost MeSH
- oxidace-redukce MeSH
- penicilinasa biosyntéza izolace a purifikace metabolismus MeSH
- substrátová specifita MeSH
- vodík metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- penicilinasa MeSH
- vodík MeSH
