High titers of anti-carbonic anhydrase I (anti-CA I) autoantibodies were detected in the sera of patients with malignancies who developed an aplastic anemia-like (AA-like) syndrome after a high-dose therapy (HDT) and autologous stem cell transplantation (ASCT). It was found, that the presence of these anti-CA I autoantibodies is associated with spontaneous tumor regression. The main immunodominant epitopes of carbonic anhydrase isoform I (CA I) have previously been identified using epitope extraction technique in combination with mass spectrometric detection and bioinformatic verification. Similarly, the sera of patients with bona fide aplastic anemia (AA) who poorly responded to immunosuppressive treatment with anti-thymocyte globulin (ATG) demonstrated high titers of anti-CA I antibodies. In order to reveal differences between these antibodies, we applied the same methodology of epitope mapping procedure. Surprisingly, the anti-CA I antibodies from the both groups of patients compatibly recognized the same four candidate CA I epitopes--DGLAV, NVGHS, SLKPI, SSEQL. This finding may indicate common pathophysiological mechanisms in these two syndromes. However, at this moment it remains unresolved if anti-CA I antibodies are implicated in marrow or tumor suppression or are just an epi-phenomenon.
- Klíčová slova
- Anti-CA I autoantibodies, Aplastic anemia, Carbonic anhydrase I, Epitope extraction, LC–MS/MS,
- MeSH
- aplastická anemie farmakoterapie imunologie MeSH
- autoprotilátky krev imunologie MeSH
- epitopy imunologie MeSH
- imunoglobulin G imunologie MeSH
- karboanhydrasa I imunologie MeSH
- lidé MeSH
- mapování epitopu MeSH
- nádory farmakoterapie imunologie MeSH
- transplantace kmenových buněk škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- autoprotilátky MeSH
- epitopy MeSH
- imunoglobulin G MeSH
- karboanhydrasa I MeSH
