OBJECTIVES: This study aimed to assess the biological impact of occupational exposure to diesel exhaust (DE) including DE particles (DEP) from heavy-duty diesel-powered equipment in Norwegian tunnel finishing workers (TFW). METHODS: TFW (n=69) and referents (n=69) were investigated for bulky DNA adducts (by 32P-postlabelling) and expression of microRNAs (miRNAs) (by small RNA sequencing) in peripheral blood mononuclear cells (PBMC), as well as circulating free arachidonic acid (AA) and eicosanoid profiles in plasma (by liquid chromatography-tandem mass spectrometry). RESULTS: PBMC from TFW showed significantly higher levels of DNA adducts compared with referents. Levels of DNA adducts were also related to smoking habits. Seventeen miRNAs were significantly deregulated in TFW. Several of these miRNAs are related to carcinogenesis, apoptosis and antioxidant effects. Analysis of putative miRNA-gene targets revealed deregulation of pathways associated with cancer, alterations in lipid molecules, steroid biosynthesis and cell cycle. Plasma profiles showed higher levels of free AA and 15-hydroxyeicosatetraenoic acid, and lower levels of prostaglandin D2 and 9-hydroxyoctadecadienoic acid in TFW compared with referents. CONCLUSION: Occupational exposure to DE/DEP is associated with biological alterations in TFW potentially affecting lung homoeostasis, carcinogenesis, inflammation status and the cardiovascular system. Of particular importance is the finding that tunnel finishing work is associated with an increased level of DNA adducts formation in PBMC.
- Klíčová slova
- biomonitoring, diesel fumes, particulates, polyaromatic hydrocarbons (pahs), toxicology,
- MeSH
- adukty DNA krev MeSH
- biologické markery krev MeSH
- dospělí MeSH
- inhalační expozice analýza MeSH
- látky znečišťující vzduch v pracovním prostředí analýza MeSH
- leukocyty mononukleární chemie MeSH
- lidé středního věku MeSH
- lidé MeSH
- lineární modely MeSH
- lipidy krev MeSH
- mikro RNA krev MeSH
- pracovní expozice škodlivé účinky MeSH
- průřezové studie MeSH
- stavebnictví * MeSH
- výfukové emise vozidel toxicita MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Norsko MeSH
- Názvy látek
- adukty DNA MeSH
- biologické markery MeSH
- látky znečišťující vzduch v pracovním prostředí MeSH
- lipidy MeSH
- mikro RNA MeSH
- výfukové emise vozidel MeSH
