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Autor
Adamoski, Douglas 1 Anfossi, Simone 1 Bustin, Stephen 1 Calin, George A 1 Chen, Baoqing 1 Dias, Sandra Martha Gomes 1 Dias-Neto, Emmanuel 1 Dragomir, Mihnea P 1 Fabbri, Muller 1 Ferracin, Manuela 1 Goel, Ajay 1 Hellemans, Jan 1 Huggett, Jim 1 Knutsen, Erik 1 Kubista, Mikael 1 Marsh, Ian 1 Mimori, Koshi 1 Negrini, Massimo 1 Papatriantafyllou, Maria 1 Pfaffl, Michael W 1
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Pracoviště
B's PT Government Associate Laborator... 1 Berlin Institute of Health Germany 1 Brazilian Biosciences National Labora... 1 CellCarta Zwijnaarde Belgium 1 Center for Cancer and Immunology Rese... 1 City of Hope Comprehensive Cancer Cen... 1 Department of Biology Faculty of Medi... 1 Department of Biomolecular Medicine G... 1 Department of Comprehensive Cancer Ca... 1 Department of Medical Biology Amsterd... 1 Department of Medical Biology Faculty... 1 Department of Medical and Surgical Sc... 1 Department of Molecular Diagnostics a... 1 Department of Pharmaceutics Universit... 1 Department of Radiation Oncology Stat... 1 Department of Surgery Kyushu Universi... 1 Department of Translational Molecular... 1 Division of Animal Physiology and Imm... 1 Division of Oncology Department of In... 1 Editorial Manager Molecular Oncology ... 1
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- Nejvíce citované
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Autor
Adamoski, Douglas 1 Anfossi, Simone 1 Bustin, Stephen 1 Calin, George A 1 Chen, Baoqing 1 Dias, Sandra Martha Gomes 1 Dias-Neto, Emmanuel 1 Dragomir, Mihnea P 1 Fabbri, Muller 1 Ferracin, Manuela 1 Goel, Ajay 1 Hellemans, Jan 1 Huggett, Jim 1 Knutsen, Erik 1 Kubista, Mikael 1 Marsh, Ian 1 Mimori, Koshi 1 Negrini, Massimo 1 Papatriantafyllou, Maria 1 Pfaffl, Michael W 1
- Organizace
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Pracoviště
B's PT Government Associate Laborator... 1 Berlin Institute of Health Germany 1 Brazilian Biosciences National Labora... 1 CellCarta Zwijnaarde Belgium 1 Center for Cancer and Immunology Rese... 1 City of Hope Comprehensive Cancer Cen... 1 Department of Biology Faculty of Medi... 1 Department of Biomolecular Medicine G... 1 Department of Comprehensive Cancer Ca... 1 Department of Medical Biology Amsterd... 1 Department of Medical Biology Faculty... 1 Department of Medical and Surgical Sc... 1 Department of Molecular Diagnostics a... 1 Department of Pharmaceutics Universit... 1 Department of Radiation Oncology Stat... 1 Department of Surgery Kyushu Universi... 1 Department of Translational Molecular... 1 Division of Animal Physiology and Imm... 1 Division of Oncology Department of In... 1 Editorial Manager Molecular Oncology ... 1
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- Untergasser, Andreas
- Hellemans, Jan
- Pfaffl, Michael W
- Ruijter, Jan M
- van den Hoff, Maurice J B
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Dragomir, Mihnea P
Autor Dragomir, Mihnea P Institute of Pathology, Berlin Institute of Health, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Germany Berlin Institute of Health, Germany German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Heidelberg, Germany
- Adamoski, Douglas
- Dias, Sandra Martha Gomes
- Reis, Rui Manuel
- Ferracin, Manuela
PubMed
36916500
PubMed Central
PMC10158759
DOI
10.1002/1878-0261.13418
Knihovny.cz E-zdroje
Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT-qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
- Klíčová slova
- RNA, RT-qPCR, accuracy, quantification,
- MeSH
- kvantitativní polymerázová řetězová reakce metody MeSH
- lidé MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- reprodukovatelnost výsledků MeSH
- RNA * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- RNA * MeSH
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