Plants of the genus Pleione, originating from hobby growers in the Netherlands and in the Czech Republic, were conspicuous for viral infection, showing symptoms of leaf mosaic or flower breaking. Using Sanger and high throughput sequencing, the full genome sequence of a novel potyvirus was obtained from sequencing data. The genome sequence was annotated and compared to the genome of other potyviruses. The virus was experimentally transmitted by aphids into Pleione and Chenopodium quinoa plants. The name Pleione flower breaking virus (PlFBV) was suggested for the new virus. The presence of the virus was confirmed using RT-PCR, with newly designed primers targeting this new species. The incidence of the virus was contrasted between both countries and might have been influenced by the growth conditions and the exposure of the plants to aphids.
- Klíčová slova
- Aphid transmission, Electron microscopy, High-throughput sequencing, Next generation sequencing, Pleione flower breaking virus, Potyvirus,
- MeSH
- anotace sekvence MeSH
- Chenopodium quinoa virologie MeSH
- hmyz - vektory MeSH
- incidence MeSH
- mšice MeSH
- nemoci rostlin virologie MeSH
- Orchidaceae virologie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- Potyvirus klasifikace genetika izolace a purifikace MeSH
- přenos infekční nemoci MeSH
- sekvenční analýza DNA MeSH
- sekvenování celého genomu MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Nizozemsko MeSH
