The endorsement of conspiracy theories may be increased by subjectively perceived stress. Yet, it is not known whether this correlation is caused by the effects of the acute stress reaction on the brain or other psychological, social, or methodological factors. The effect of an experimentally induced acute stress reaction on conspiracy thinking was tested on a sample (n = 115) of students of medicine. Although the stress procedure caused a substantial increase in salivary cortisol, there was no significant effect on endorsing conspiracy theories or adopting conspiracy interpretations of novel information. The results confirmed no effect of the acute stress reaction on conspiracy thinking, suggesting it may be absent or weaker than expected. The study demonstrated the viability of psychophysiological experimental design in conspiracy research and may inspire further examination of the physiological mechanisms underlying susceptibility to conspiracy theories.

• Klíčová slova
• Conspiracy theories, Maastricht Acute Stress Test, cortisol, stress,
• MeSH
• dospělí MeSH
• hydrokortison analýza   metabolismus   MeSH
• lidé MeSH
• mladý dospělý MeSH
• psychický stres * psychologie   MeSH
• sliny chemie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hydrokortison MeSH

Early life experiences have long-lasting effects on behaviour and physiology, influencing development of adaptive natural behaviours. Enriching farmed environments encourages expression of natural behaviours in captive fish, promoting positive animal welfare, important for conducting valid and reproducible research and informing better management practices. Using juvenile Atlantic Salmon (Salmo salar), we tested whether provision of environmental enrichment in early life improves welfare. Welfare indicators were measured comparing enriched to non-enriched tanks. Morphological (fin damage and body condition), physiological (plasma cortisol) and behavioural traits (activity, group cohesion, and neophobia) were recorded. Molecular expression of brain mRNA transcripts related to stress response, neuroplasticity and serotonergic system was analysed. Environmental enrichment did not affect morphological welfare indicators, activity, or cortisol. Enriched fish were more cohesive than non-enriched fish, less neophobic, with higher serotonergic turnover, suggesting enrichment mitigates against stress, promoting positive emotional states. Genes related to neuronal development and activity (bdnf and ndf1), cellular stress (hsp90 and hsp70), and serotonin synthesis (tph2) increased in enriched fish following stress, enhancing cognitive function. Our findings suggest early life environmental enrichment is advantageous for positive animal welfare by improving emotional states in captive environments, ensuring animals are free of negative experiences and able to access positive ones.

• Klíčová slova
• Cognitive abilities, Fish welfare, Neurogenesis, Social behaviour, Structural enrichment,
• MeSH
• chování zvířat MeSH
• fyziologický stres MeSH
• hydrokortison krev   MeSH
• pohoda zvířat * MeSH
• Salmo salar * fyziologie   růst a vývoj   MeSH
• serotonin metabolismus   MeSH
• vodní hospodářství * metody   MeSH
• životní prostředí * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hydrokortison MeSH
• serotonin MeSH

BACKGROUND: The frequent presence of hunters in hunting areas may have a negative impact on wild animals, which may disturb their welfare. Stressors may long affect individual animal species in different ways. Therefore, the study aimed to compare cortisol level in the hair of male mouflons, red deer, and wild boars harvested at the end of the stalking hunting season depending on the age and carcass mass. The cortisol level was determined using the DetectX® Cortisol ELISA Kits. RESULTS: The concentration of cortisol in the hair of mouflons was almost six times lower than that of red deer and wild boars. Carcass mass and age researched animals did not affect cortisol levels. CONCLUSIONS: Stalking hunts most likely do not impact the welfare of big game. There is probably habituation to long-term stress in the animals studied. Mouflons have the ability to respond effectively to specific stressors and are more resistant to long-term stress.

• Klíčová slova
• Hunting activities, Stress hormone, Welfare,
• MeSH
• fyziologický stres * MeSH
• hydrokortison * analýza   MeSH
• pohoda zvířat MeSH
• roční období * MeSH
• Sus scrofa * fyziologie   MeSH
• vlasy, chlupy * chemie   MeSH
• vysoká zvěř * fyziologie   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hydrokortison * MeSH

The purpose of this study was to determine the effects of resistance training (RT) alongside creatine-hydrochloride (Cr-HCl) or creatine monohydrate (CrM) supplementation on anabolic/catabolic hormones, strength, and body composition. Forty participants with an age range of 18-25 years were randomly divided into four groups (n=10): RT+Cr-HCl (0.03 g.kg-1 of body mass), RT+CrM-loading phase (CrM-LP) (0.3 g.kg-1 of body mass for five days (loading) and 0.03 g.kg-1 body mass for 51 days (maintenance)), RT+CrM-without loading phase (CrM-WLP) (0.03 g.kg-1 body mass), and RT+placebo (PL). The participants consumed supplements and performed RT with an intensity of 70-85 % 1RM for eight weeks. Before and after the training and supplementation period, strength (1RM), body composition (percent body fat (PBF), skeletal muscle mass (SMM), muscular cross-sectional area (MCSA)) and serum levels of testosterone, growth hormone (GH), insulin-like growth factor-1 (IGF-1), cortisol, adrenocorticotropic hormone (ACTH), follistatin and myostatin were measured. The results showed that in the supplementation groups, strength, arm and thigh MCSA, and SMM significantly increased, and PBF significantly decreased (P

• MeSH
• dospělí MeSH
• hormony * krev   MeSH
• hydrokortison krev   MeSH
• insulinu podobný růstový faktor I metabolismus   MeSH
• kosterní svaly metabolismus   účinky léků   MeSH
• kreatin * farmakologie   MeSH
• lidé MeSH
• lidský růstový hormon krev   MeSH
• mladiství MeSH
• mladý dospělý MeSH
• odporový trénink * metody   MeSH
• potravní doplňky * MeSH
• složení těla * účinky léků   MeSH
• svalová síla * účinky léků   MeSH
• testosteron krev   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• hormony * MeSH
• hydrokortison MeSH
• insulinu podobný růstový faktor I MeSH
• kreatin * MeSH
• lidský růstový hormon MeSH
• testosteron MeSH

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) mainly afflicting young women. Various steroids can influence the onset and development of the disease or, on the contrary, mitigate its course; however, a systematic review of steroidomic changes in MS patients is lacking. Based on the gas chromatography tandem mass spectrometry (GC-MS/MS) platform and, in the case of estradiol, also using immunoassay, this study performed a comprehensive steroidomic analysis in 25 female MS patients aged 39(32, 49) years compared to 15 female age-matched controls aged 38(31, 46) years. A significant trend towards higher ratios of conjugated steroids to their unconjugated counterparts was found in patients, which is of particular interest in terms of the balance between excitatory and inhibitory steroid modulators of ionotropic receptors. Patients showed altered metabolic pathway to cortisol with decreased conversion of pregnenolone to 17-hydroxypregnenolone and 17-hydroxypregnenolone to 17-hydroxyprogesterone and increased conversion of 17-hydroxypregnenolone to dehydroepiandrosterone (DHEA), resulting in lower levels of 17-hydroxyprogesterone, as well as indications of impaired conversion of 11-deoxy-steroids to 11β-hydroxy-steroids but reduced conversion of cortisol to cortisone. Due to over-activation of hypothalamic-pituitary-adrenal axis (HPAA), however, cortisol and cortisone levels were higher in patients with indications of depleted cortisol synthesizing enzymes. Patients showed lower conversion of DHEA to androstenedione, androstenedione to testosterone, androstenedione to estradiol in the major pathway, and testosterone to estradiol in the minor pathway for estradiol synthesis at increased conversion of androstenedione to testosterone. They also showed lower conversion of immunoprotective Δ5 androstanes to their more potent 7α/β-hydroxy metabolites and had lower circulating allopregnanolone and higher ratio 3β-hydroxy-steroids to their neuroprotective 3α-hydroxy-counterparts.

• Klíčová slova
• GC-MS/MS, differential diagnostics, multiple sclerosis, multivariate statistics, steroidomics,
• MeSH
• dospělí MeSH
• hydrokortison metabolismus   krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
• roztroušená skleróza * metabolismus   krev   MeSH
• steroidy metabolismus   MeSH
• studie případů a kontrol MeSH
• tandemová hmotnostní spektrometrie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hydrokortison MeSH
• steroidy MeSH

Hyperthermia along with hydrocortisone (HC) are proven teratogens that can negatively influence embryo development during early pregnancy. Proliferation of cells is one of the main developmental processes during the early embryogenesis. This study was focused on testing the effect of elevated temperature and HC addition on proliferation of cells in in vitro cultures. The V79-4 cell line was treated with HC and cultured in vitro at 37 °C or 39 °C, respectively. To reveal the effect of both factors, the proliferation of cells cultured under different conditions was evaluated using various approaches (colony formation assay, generation of growth curves, computation of doubling times, and mitotic index estimation). Our results indicate that a short-term exposure to elevated temperature slightly stimulates and a long-term exposure suppresses cell proliferation. However, HC (0.1 mg/ml) acts as a stimulator of cell proliferation. Interestingly, the interaction of HC and long-term elevated temperature (39 °C) exposure results in at least partial compensation of the negative impact of elevated temperature by HC addition and in higher proliferation if compared with cells cultured at 39 °C without addition of HC.

• Klíčová slova
• Corticosteroids, Fibroblasts, Hyperthermia, Interaction, Proliferation, in vitro,
• MeSH
• Cricetulus MeSH
• fibroblasty * účinky léků   cytologie   metabolismus   MeSH
• hydrokortison * farmakologie   MeSH
• kultivované buňky MeSH
• proliferace buněk * účinky léků   MeSH
• teplota MeSH
• vysoká teplota MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hydrokortison * MeSH

Roma living in marginalised communities are among the most disadvantaged groups in Slovakia. Socioeconomic disadvantage is associated with higher hair cortisol concentrations (HCC), including in parents. The aim of this study is therefore to assess differences in HCC, reflecting the levels of stress, between mothers living in MRCs and from the majority population, to assess the association of socioeconomic disadvantage with HCC, and whether disadvantage mediates the MRC/majority differences in HCC. Participants were mothers of children aged 15-18 months old living in MRCs (N=61) and from the Slovak majority population (N=90). During preventive paediatric visits, visits at community centres and home visits, hair samples and data by questionnaire were collected. HCC differed significantly between mothers living in MRCs and mothers from the majority population, with the mean HCC value being twice as high in mothers living in MRCs (22.98 (95% confidence interval, CI, 15.70-30.30) vs. 11.76 (8.34-15.20), p<0.05). HCC was significantly associated with education, household equipment and household overcrowding, but not with billing, socioeconomic stress and social support. The difference in HCC between mothers living in MRCs and mothers from the majority population was partially mediated by poor house equipment, such as no access to running water, no flushing toilet or no bathroom (the indirect effect of B=7.63 (95% CI: 2.12-13.92)). Practitioners and policymakers should be aware of high stress levels among mothers living in MRCs and aim at enhancing their living and housing conditions.

• Klíčová slova
• Hair cortisol concentrations, Housing conditions, Marginalized Roma communities, Mothers of small children, Parental stress, Socioeconomic disadvantage,
• MeSH
• dospělí MeSH
• hydrokortison * analýza   metabolismus   MeSH
• kojenec MeSH
• lidé MeSH
• matky * psychologie   MeSH
• psychický stres * metabolismus   MeSH
• Romové * MeSH
• sociální marginalizace psychologie   MeSH
• sociální opora MeSH
• socioekonomické faktory * MeSH
• socioekonomické nerovnosti ve zdraví MeSH
• vlasy, chlupy * chemie   MeSH
• zranitelné populace psychologie   MeSH
• Check Tag
• dospělí MeSH
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Slovenská republika MeSH
• Názvy látek
• hydrokortison * MeSH

Schizophrenia is associated with numerous abnormalities, including imbalances in all hormonal axes, among which steroids play a major role. Steroidomic studies therefore represent a promising tool for early diagnosis and appropriate treatment of schizophrenia. A total of 51 adult male schizophrenics aged 27 (22, 34) years (shown as median with quartiles) and 16 healthy controls (HCs) aged 28 (25, 32) years were enrolled into this study. Our results showed the effective differentiation of men with schizophrenia from controls based on steroidomic profiles. We also found an altered metabolic pathway from pregnenolone and its sulfate (PREG/S) to cortisol in schizophrenics with several metabolic bottlenecks such as lower PREG levels due to increased PREG sulfation and/or suppressed PREGS desulfation and attenuated conversion of 17-hydroxy-PREG to 17-hydroxy-progesterone, as well as the results suggestive of suppressed CYP11B1 activity. In contrast, steroid molar ratios suggested two counterregulatory steps involving increased conversion of PREG/S to 17-hydroxy-PREG/S and decreased conversion of cortisol to cortisone, which may maintain unchanged basal cortisol levels but may not ensure a sufficient cortisol response to stress. Our data also indicated a trend to higher 7α-, 7β-, and 16α-hydroxylation that may counteract the autoimmune complications and proinflammatory processes accompanying schizophrenia. Finally, a possible suppression of HSD17B3 activity was suggested, resulting in decreased circulating testosterone levels with increased androstenedione levels.

• Klíčová slova
• GC-MS/MS, differential diagnostics, multivariate statistics, schizophrenia, steroidomics,
• MeSH
• dospělí MeSH
• hydrokortison metabolismus   krev   MeSH
• lidé MeSH
• mladý dospělý MeSH
• pregnenolon metabolismus   krev   MeSH
• schizofrenie * metabolismus   MeSH
• steroidy metabolismus   MeSH
• studie případů a kontrol MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hydrokortison MeSH
• pregnenolon MeSH
• steroidy MeSH

This study aimed to investigate the effects of a single bench press (BP) vs. leg press (LP) resistance training sessions on testosterone, cortisol, C-reactive protein (CRP) interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) concentrations, and creatine kinase (CK) activity in strength-trained males. Eleven strength-trained males participated in a cross-over randomized trial, undergoing two experimental sessions each consisting of five sets of the BP or the LP exercise to volitional failure with a load corresponding to 50% of one-repetition maximum. Blood samples were taken at baseline (BA), immediately post (POST), and 1 h after the cessation of exercise (POST-1). A significant increase in IL-6 concentration from BA to POST-1 was observed during the LP condition (p = 0.004; effect size [ES] = 0.64). Additionally, a significant main effect of time was found for increasing testosterone concentrations from BA to POST exercise (p = 0.014; ES = 0.25). A significantly lower cortisol concentration at POST-1 compared to POST (p = 0.001; ES = 1.02) was noted in the BP condition. Furthermore, a significantly lower cortisol concentration was found at POST-1 in the BP compared to the LP condition (p = 0.022; ES = 1.3). A significant increase in CK activity was reported from BA to POST (p = 0.024; ES = 0.69) and POST-1 (p = 0.045; ES = 0.55) during the LP condition, and from BA to POST-1 (p = 0.014; ES = 0.96) during the BP condition. No significant differences were found in the CRP (p = 0.659) and TNF-α concentrations (p = 0.487). These results suggest that the amount of muscle mass engaged during the resistance exercise may influence the changes in IL-6 and cortisol concentrations. Larger muscle groups, as engaged in the LP, more likely lead to elevated concentrations of IL-6 myokine.

• Klíčová slova
• CRP, TNF-α, bench press, cortisol, creatine kinase, interleukin, myokine, testosterone,
• MeSH
• C-reaktivní protein metabolismus   MeSH
• dospělí MeSH
• hydrokortison * krev   MeSH
• interleukin-6 * krev   MeSH
• klinické křížové studie MeSH
• kreatinkinasa krev   MeSH
• lidé MeSH
• mladý dospělý MeSH
• odporový trénink * MeSH
• testosteron * krev   MeSH
• TNF-alfa * krev   MeSH
• zánět krev   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• C-reaktivní protein MeSH
• hydrokortison * MeSH
• interleukin-6 * MeSH
• kreatinkinasa MeSH
• testosteron * MeSH
• TNF-alfa * MeSH

A surface plasmon resonance (SPR) phenomenon implemented via D-shaped polymer optical fiber (POF) is exploited to realize cortisol biosensors. In this work, two immonosensors are designed and developed for the qualitative as well as quantitative measurement of cortisol in artificial and real samples. The performances of the POF-based biosensors in cortisol recognition are achieved using different functionalization protocols to make the same antibody receptor layer over the SPR surface via cysteamine and lipoic acid, achieving a limit of detection (LOD) of 0.8 pg/mL and 0.2 pg/mL, respectively. More specifically, the use of cysteamine or lipoic acid changes the distance between the receptor layer and the SPR surface, improving the sensitivity at low concentrations of about one order of magnitude in the configuration based on lipoic acid. The LODs of both cortisol biosensors are achieved well competitively with other sensor systems but without the need for amplification or sample treatments. In order to obtain the selectivity tests, cholesterol and testosterone were used as interfering substances. Moreover, tests in simulated seawater were performed for the same cortisol concentration range achieved in buffer solution to assess the immunosensor response to the complex matrix. Finally, the developed cortisol biosensor was used in a real seawater sample to estimate the cortisol concentration value. The gold standard method has confirmed the estimated cortisol concentration value in real seawater samples. Liquid-liquid extraction was implemented to maximize the response of cortisol in liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis.

• MeSH
• biosenzitivní techniky * metody   MeSH
• hydrokortison * analýza   MeSH
• limita detekce MeSH
• mořská voda * analýza   MeSH
• optická vlákna MeSH
• polymery chemie   MeSH
• povrchová plasmonová rezonance * metody   MeSH
• vodní hospodářství * metody   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• hydrokortison * MeSH
• polymery MeSH