Plants with anticancer properties are considered as cancer preventive and treatment sources, due to their some biological effects. Apoptosis induction and anti-proliferative effects of Baneh extract on various cancer cell lines have been reported. Hence, this study was designed to evaluate the cytotoxic effects of this fruit on KB and human gingival fibroblast cell lines (HGF). KB and HGF cells were treated with various concentrations of ethanolic Baneh extract and cisplatin as positive control. Cytotoxic activity and apoptosis induction were investigated using WST-1 and Annexin V assays. Data were analyzed using ANOVA and student's t-tests. IC50 after 24 and 48 hours treatment were respectively 2.6 and 1 mg/mL for KB cell line, and 1.5 and 1.6 mg/mL for HGF cell. During 48 hours Baneh extract induced apoptosis without significant necrosis, in a time- and dose-dependent manner. The induction of apoptosis in KB cells was significantly higher than HGF. It seems that ethanolic extract of Baneh contains compounds that can suppress KB cell growth through the induction of apoptosis. Within 48 hours, less cytotoxic effects were observed on normal fibroblast cells; therefore, it might be a potential anticancer agent.
- Klíčová slova
- apoptosis, cell line, cytotoxicity, fibroblast, pistacia, squamous cell carcinoma,
- MeSH
- apoptóza účinky léků MeSH
- fytogenní protinádorové látky farmakologie MeSH
- gingiva cytologie účinky léků patologie MeSH
- KB buňky MeSH
- lidé MeSH
- nádory děložního čípku farmakoterapie patologie MeSH
- Pistacia chemie MeSH
- proliferace buněk účinky léků MeSH
- regulace genové exprese u nádorů účinky léků MeSH
- rostlinné extrakty farmakologie MeSH
- spinocelulární karcinom farmakoterapie patologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- fytogenní protinádorové látky MeSH
- rostlinné extrakty MeSH
Effective and controlled drug delivery systems with on-demand release and targeting abilities have received enormous attention for biomedical applications. Here, we describe a novel enzyme-based cap system for mesoporous silica nanoparticles (MSNs) that is directly combined with a targeting ligand via bio-orthogonal click chemistry. The capping system is based on the pH-responsive binding of an aryl-sulfonamide-functionalized MSN and the enzyme carbonic anhydrase (CA). An unnatural amino acid (UAA) containing a norbornene moiety was genetically incorporated into CA. This UAA allowed for the site-specific bio-orthogonal attachment of even very sensitive targeting ligands such as folic acid and anandamide. This leads to specific receptor-mediated cell and stem cell uptake. We demonstrate the successful delivery and release of the chemotherapeutic agent Actinomycin D to KB cells. This novel nanocarrier concept provides a promising platform for the development of precisely controllable and highly modular theranostic systems.
- MeSH
- aktivní transport MeSH
- buněčné linie MeSH
- daktinomycin aplikace a dávkování farmakokinetika MeSH
- HeLa buňky MeSH
- karboanhydrasa II chemie genetika metabolismus MeSH
- KB buňky MeSH
- lékové transportní systémy * MeSH
- léky s prodlouženým účinkem MeSH
- lidé MeSH
- myši MeSH
- nanočástice * chemie MeSH
- oxid křemičitý MeSH
- proteinové inženýrství MeSH
- protinádorové látky aplikace a dávkování farmakokinetika MeSH
- receptory léků chemie genetika metabolismus MeSH
- uvolňování léčiv MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- daktinomycin MeSH
- karboanhydrasa II MeSH
- léky s prodlouženým účinkem MeSH
- oxid křemičitý MeSH
- protinádorové látky MeSH
- receptory léků MeSH
In mice local or systemic administration of fluoride (5-25 mg NaF/kg body weight) during the proliferative phase of bone formation (up to 20 days) has no effect on the yield of bone formed either by local stimulation of periosteal membrane by Moloney sarcoma virus-induced tumour or on bone induced heterotopically by human KB cells. The lack of stimulatory activity of fluoride on rapidly induced osteogenesis in mice is in agreement with recent reports which show that fluoride is not a potent mitogen for human osteoblasts grown in vitro.
- MeSH
- experimentální sarkom metabolismus MeSH
- fluorid sodný aplikace a dávkování farmakologie MeSH
- inbrední kmeny myší MeSH
- KB buňky MeSH
- kosti a kostní tkáň účinky léků MeSH
- lidé MeSH
- Moloneyho virus myšího sarkomu MeSH
- myši MeSH
- nádory kostí patologie MeSH
- okostice účinky léků MeSH
- osteogeneze účinky léků MeSH
- transplantace nádorů MeSH
- virová transformace buněk MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fluorid sodný MeSH
