The primarily bat-associated argasid tick, Secretargas transgariepinus (White, 1846), is a member of the Afrotropical and southern Palaearctic fauna. Probably because of its secretive life style, little is known about this species and records of its collection are scant. Based on morphological revisions of the available specimens, we report new Middle Eastern records for this tick species that had been misidentified as other bat-associated argasid taxa. These specimens are larvae from three localities, and represent the first records of S. transgariepinus from two countries: one larva from Sabratha (Libya) was collected from an unidentified bat species (possibly Eptesicus isabellinus), seven larvae from Azraq-Shishan (Jordan), and 78 larvae from Shamwari (Jordan) were all collected from Otonycteris hemprichii. Twenty larvae from Shamwari were also tested for the presence of both, viral or bacterial microorganisms by PCR. Three ticks were found to be infected with the Murid gammaherpesvirus 68 (MHV-68), one with Borrelia burgdorferi sensu lato, and four with a Rickettsia sp. closely related to Rickettsia slovaca. The findings represent a first evidence for the occurrence of these possible pathogens in S. transgariepinus.
- Klíčová slova
- Arid area, Main host, Microorganism, Middle East, Nidicolous, Secretive bat-argas, Virus,
- MeSH
- Argasidae * mikrobiologie MeSH
- Chiroptera * parazitologie MeSH
- larva * mikrobiologie MeSH
- polymerázová řetězová reakce MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Jordánsko MeSH
- Libye MeSH
Two gonad-infecting species of Philometra Costa, 1845 (Nematoda, Philometridae) were recorded for the first time from marine perciform fishes off Tunisia and Libya: Philometra rara n. sp. from the rare, deep-water Haifa grouper Hyporthodus haifensis (Serranidae) off Libya and Philometra saltatrix Ramachandran, 1973 from the bluefish Pomatomus saltatrix (Pomatomidae) off Tunisia. Identification of both fish species was confirmed by molecular barcoding. Light and scanning electron microscope studies of Ph. rara n. sp. showed that it is characterized by the length of spicules (216-219 μm) and the gubernaculum (90-93 μm), the gubernaculum/spicules length ratio (1:2.32-2.43), and mainly by the shape and structure of the distal end of the gubernaculum (shovel-shaped with a wide median smooth field in dorsal view), appearing as having a dorsal protuberance in lateral view, and by the structure of the male caudal mound (dorsally interrupted); large subgravid females (70-137 mm long) are characterized by the presence of four oval submedian cephalic elevations, each of them bearing a pair of cephalic papillae of the outer circle. The finding of Ph. saltatrix off Tunisia confirms that this species is widespread throughout the Mediterranean region. A molecular analysis of our Ph. saltatrix specimens and other available philometrid cytochrome c oxidase 1 (COI) sequences showed that most species have robust clades. Sequences of Ph. saltatrix from Tunisia diverge from Ph. saltatrix from Brazil and the USA, suggesting that speciation is currently occurring between populations from both sides of the Atlantic Ocean.
Deux espèces de Philometra Costa, 1845 (Nematoda, Philometridae) parasites des gonades sont, pour la première fois, signalées de poissons perciformes marins au large de la Tunisie et de la Libye : Philometra rara n. sp. (Serranidae) du mérou de Haïfa Hyporthodus haifensis, une espèce rare, au large de la Libye et Philometra saltatrix Ramachandran, 1973, du tassergal Pomatomus saltatrix (Pomatomidae) au large de la Tunisie. L’identification des deux espèces de poissons a été confirmée par barcode moléculaire. Des études en microscopie photonique et microscopie électronique à balayage de Ph. rara n. sp. ont montré que l’espèce se caractérise par la longueur des spicules (216-219 μm) et du gubernaculum (90-93 μm), le rapport gubernaculum/spicules (1: 2.32-2.43) et principalement par la forme et la structure de l’extrémité distale du gubernaculum (en forme de pelle avec un large champ médian lisse en vue dorsale), apparaissant comme ayant une protubérance dorsale en vue latérale, et par la structure du monticule caudal mâle (interrompu dorsalement); les grandes femelles subgravides (70-137 mm de long) se caractérisent par la présence de quatre élévations céphaliques ovales sous-médianes, chacune portant une paire de papilles céphaliques sur le cercle extérieur. La découverte de Ph. saltatrix au large de la Tunisie confirme que cette espèce est répandue dans la région méditerranéenne. Une analyse moléculaire de nos spécimens de Ph. saltatrix et d’autres séquences disponibles de COI de Philometridae montre que la plupart des espèces présentent des clades robustes. Les séquences de Ph. saltatrix en provenance de Tunisie divergent de Ph. saltatrix du Brésil et des États-Unis, ce qui suggère qu’une spéciation se produit actuellement entre les populations des deux côtés de l’océan Atlantique.
- MeSH
- Dracunculoidea anatomie a histologie klasifikace genetika ultrastruktura MeSH
- infekce hlísticemi řádu Spirurida epidemiologie parazitologie veterinární MeSH
- mikroskopie elektronová rastrovací MeSH
- nemoci ryb epidemiologie parazitologie MeSH
- Percoidea genetika parazitologie MeSH
- prevalence MeSH
- ryby MeSH
- taxonomické DNA čárové kódování veterinární MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Libye MeSH
- Středozemní moře MeSH
- Tunisko MeSH
AIMS: This cross-sectional survey assessed and compared by gender the levels and correlates of achieving the international guidelines recommendations on five forms of physical activity (PA) (walking, moderate PA, vigorous PA, moderate or vigorous PA- MVPA, and muscle-strengthening PA). We examined the associations between achieving the international guidelines recommendations on five forms of PA and a variety of sociodemographic, health and academic performance variables. METHODS: Data was collected across nine Libyan universities (2008-2009, representative sample of 1,300 undergraduates). A self-administered questionnaire assessed frequency and duration of PA/week for each form of PA. Cut-offs for recommended guidelines were set according to the American Heart Association. RESULTS: About 43.7% of students achieved the guidelines for muscle strengthening PA, 20.7% for walking, 11.2% for vigorous PA, 28.5% for MVPA, and 4.8% for moderate PA (excluding walking). Binary logistic regression showed that except for walking, males were consistently associated with achieving the guidelines recommendations for all other forms of PA. Students with higher health awareness were more likely to achieve the guidelines for vigorous PA and muscle strengthening PA. Better academic performance was associated with higher levels of muscle strengthening PA. Students at institutions in the East of Libya were less likely to achieve the guidelines for walking, vigorous PA or muscle strengthening PA as compared to students from the North. Students from the West of Libya were also less likely to walk or to engage in moderate PA than their peers from the North. CONCLUSIONS: Overall, relatively low proportions of students achieved the recommended guidelines of PA. Female students were particularly at risk for low level of PA. Attention is required to national policies that promote active living, along with addressing the cultural, geographic and other barriers to engagement of young adult males and females in PA.
- MeSH
- charakteristiky bydlení statistika a číselné údaje MeSH
- cvičení * MeSH
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- průřezové studie MeSH
- sexuální faktory MeSH
- studenti statistika a číselné údaje MeSH
- univerzity * MeSH
- zdraví - znalosti, postoje, praxe * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- Geografické názvy
- Libye MeSH
BACKGROUND: Diagnosis of atypical and silent forms of coeliac disease (CD) is important because of its serious complications. Increased prevalence of coeliac disease worldwide in individuals with type 1 diabetes mellitus (DM) was described. There are no data on the prevalence of CD in the Libyan population and Libyan DM patients. The aim of this study was to test the occurrence of CD-related markers in a group of Libyan children with DM. METHODS: A cohort of 234 Libyan children with DM (121 males and 113 females) aged between 2 and 25 years and 50 healthy school children were screened for CD using the enzyme-linked immunosorbent assay (ELISA) for IgA and IgG antigliadin (AGA), anti-tissue transglutaminase (tTG), and anticalreticulin antibodies. An IgA antiendomysial antibody (EmA) was determined by immunofluorescence. RESULTS: Fifty patients (21.3%) were positive for IgA- and/or IgG-AGA, tTG, and anticalreticulin antibodies. Nineteen of these patients were EmA positive and seven were EmA negative. From the EmA negative patients we found that five sera with IgA deficiency had high IgG class in antigliadin, anti-tissue transglutaminase, and anticalreticulin antibodies. All these patients underwent intestinal biopsy. Twenty-four had clear histological (atrophy) evidence of CD including the EmA negative patients with IgA deficiency; prevalence of CD in this study was thus 10.3%. CONCLUSION: The prevalence of CD in diabetic children in Libya was found to be higher than in several European countries. Serological markers are useful for identifying DM patients who should undergo a small intestinal biopsy.
- MeSH
- biopsie MeSH
- celiakie komplikace epidemiologie imunologie MeSH
- diabetes mellitus 1. typu komplikace epidemiologie imunologie MeSH
- dítě MeSH
- dospělí MeSH
- gliadin imunologie MeSH
- imunoglobulin A krev MeSH
- imunoglobulin G krev MeSH
- kalretikulin imunologie MeSH
- kohortové studie MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- prevalence MeSH
- střevní sliznice imunologie patologie MeSH
- transglutaminasy imunologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Libye epidemiologie MeSH
- Názvy látek
- gliadin MeSH
- imunoglobulin A MeSH
- imunoglobulin G MeSH
- kalretikulin MeSH
- transglutaminasy MeSH
BACKGROUND: There are, as yet, no data available about the incidence of celiac disease in Libya. The aim of this study was to test the occurrence of serologic markers in a group of Libyan children with positive clinical and histologic findings indicative of celiac disease diagnosis. METHODS: Thirty-nine children with untreated celiac disease and 50 healthy school children, all younger than 14 years, were included in the study. Enzyme-linked immunosorbent assay for immunoglobulin G and immunoglobulin A (IgA) antigliadin, antitissue transglutaminase, and anticalreticulin antibodies was used to evaluate the serologic markers of the celiac patients. Immunoglobulin A antiendomysial antibodies were detected by indirect immunofluorescence using human umbilical cord tissue. RESULTS: Clinical symptoms at presentation were weight loss (82%), abdominal distension (61.5%), diarrhea or steatorrhea (59%), pallor (41%), abdominal pain (20.5%), constipation (15%), vomiting (10%), and short stature (7.7%). Most of these symptoms disappeared after introduction of a gluten-free diet. Of 39 patients, only 23 (59%) were endomysium positive and positive also in all other serologic markers. The second group of patients with positive clinical and biopsy findings but antiendomysial antibody-negative findings was subdivided into two subgroups according to the IgA antigliadin antibody results. Individuals in the IgA gliadin-negative subgroup also lacked IgA autoantibodies. CONCLUSIONS: The authors' findings stress the importance of serologic testing not only for screening but also for confirmation of celiac disease.
- MeSH
- autoprotilátky krev imunologie MeSH
- biologické markery krev MeSH
- celiakie krev diagnóza epidemiologie imunologie MeSH
- dieta MeSH
- dítě MeSH
- ELISA MeSH
- fluorescenční protilátková technika nepřímá MeSH
- gliadin imunologie MeSH
- gluteny aplikace a dávkování MeSH
- imunoglobulin A imunologie MeSH
- imunoglobulin G imunologie MeSH
- incidence MeSH
- jejunum patologie MeSH
- kalretikulin MeSH
- lidé MeSH
- mladiství MeSH
- proteiny vázající vápník imunologie MeSH
- ribonukleoproteiny imunologie MeSH
- transglutaminasy imunologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Libye epidemiologie MeSH
- Názvy látek
- autoprotilátky MeSH
- biologické markery MeSH
- gliadin MeSH
- gluteny MeSH
- imunoglobulin A MeSH
- imunoglobulin G MeSH
- kalretikulin MeSH
- proteiny vázající vápník MeSH
- ribonukleoproteiny MeSH
- transglutaminasy MeSH
The authors present a report on the first detection of the strain Dermatophilus congolensis in Czechoslovakia. The strain was isolated from ulcerations on the dorsal part of the foot of a 47-year-old patient who previously worked for several years in Libya. Based on examinations of smears from cultures of an isolated strain and four strains from a collection, from experimental dermatofilosis of a rabbit the authors describe the micromorphology of strains of D. congolensis under a light and electron microscope (transmission an and raster microscope). They describe the colonial morphology, haemolytic properties and in particular detail the haemolytic interaction of strains of D. congolensis with Streptococcus agalactiae, Rhodococcus equi and with strains of S. aureus (monoproducers of beta-haemolysin and delta-haemolysin resp.). They assessed the sensitivity of strains of D. congolensis to some staphylococcal bacteriocin-like substances.
- MeSH
- Actinomycetales izolace a purifikace fyziologie MeSH
- bakteriální nemoci kůže diagnóza epidemiologie MeSH
- cestování MeSH
- infekce bakteriemi řádu Actinomycetales diagnóza epidemiologie MeSH
- králíci MeSH
- lidé středního věku MeSH
- lidé MeSH
- vředy na noze (od hlezna dolů) mikrobiologie patologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- kazuistiky MeSH
- Geografické názvy
- Československo epidemiologie MeSH
- Libye MeSH
The author describes the social and medical situation in the Libyan Arab Peoples Socialist Djamahiriji. He presents an epidemialogical study of the prevalence of some malocclusions. From the results it is apparent that it is necessary to introduce systematic orthodontic care which does not exist so far in the country.
- MeSH
- lidé MeSH
- malokluze epidemiologie MeSH
- zdravotnické služby - potřeby a požadavky MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Geografické názvy
- Libye epidemiologie MeSH
In a catchment area with population of approximately 60,000 in Libya for a period of two years and three months a group of 38 children aged 7-12 years with the diagnosis of rheumatic fever was followed up. An incidence of cca 60 attacks of rheumatic fever per 100,000 children per year was recorded. The clinical picture of primary attacks did not differ from the clinical picture of primary attacks in technically advanced countries in the sixties and also the development of the finding on the heart in rheumatic patients with penicillin prophylaxis was equally favourable.
- MeSH
- cestování * MeSH
- lidé MeSH
- parazitární nemoci střev epidemiologie parazitologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Geografické názvy
- Angola etnologie MeSH
- Československo MeSH
- Korea etnologie MeSH
- Kuba etnologie MeSH
- Laos etnologie MeSH
- Libye etnologie MeSH
- Vietnam etnologie MeSH
