Spices are a rich source of vitamins, polyphenols, proteins, dietary fiber, and minerals such as calcium, magnesium, iron, and zinc, all of which play an important role in biological functions. Since ancient times, spices have been used in our kitchen as a food coloring agent. Spices like cinnamon and turmeric allegedly contain various functional ingredients, such as phenolic and volatile compounds. Therefore, this review aims to summarize the current knowledge about the nutritional profiles of cinnamon and turmeric, as well as to analyze the clinical studies on their extracts and essential oils in animals and humans. Furthermore, their enrichment applications for food products and animal feed have also been investigated in terms of safety and toxicity. Numerous studies have shown that cinnamon and turmeric have various health benefits, including the reduction of insulin resistance and insulin signaling pathways in diabetic patients, the reduction of inflammatory biomarkers, and the maintenance of gut microflora in both animals and humans. The food and animal feed industries have taken notice of these health benefits and have begun to promote cinnamon and turmeric as healthy foods. This has resulted in the development of new food products and animal feeds that contain cinnamon and turmeric as primary ingredients, which have been deemed an effective means of promoting cinnamon and turmeric's health benefits.

• Klíčová slova
• Cinnamon, enrichment, functional feed, functional food, turmeric,
• MeSH
• Curcuma * chemie   MeSH
• funkční potraviny * MeSH
• inzulinová rezistence MeSH
• koření * analýza   MeSH
• krmivo pro zvířata * analýza   MeSH
• lidé MeSH
• oleje prchavé farmakologie   MeSH
• rostlinné extrakty farmakologie   MeSH
• skořicovník ceylonský * chemie   MeSH
• střevní mikroflóra účinky léků   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• oleje prchavé MeSH
• rostlinné extrakty MeSH

The widespread use of hexachlorocyclohexanes (HCH) as pesticides has raised environmental concerns due to their persistence and toxicity. Addressing the pressing need for effective bioremediation strategies, this study explores the effects of α-, β-, δ-, and ε-HCH isomers on the growth, hormonal changes, physiological parameters and bioaccumulation in Alnus glutinosa saplings (1-year-old and 2-year-old) and bacterial communities in polluted soil. A. glutinosa saplings not only withstanded HCH exposure but also enhanced the remediation efficiency by 6.8-24.4%, suggesting an acceleration of pollutant breakdown likely mediated by root exudates positively affecting the soil microbiome. Interestingly, 1-year-old saplings demonstrated greater remediation efficiency post-pruning than unpruned 2-year-old saplings, despite the latter having a larger root biomass. The hormonal analysis indicated that HCH presence led to a reduction in abscisic acid (ABA) and an increase in jasmonic acid (JA), with the magnitude of changes being age-dependent. Salicylic acid (SA) levels increased 1-year-old and decreased in 2-year-old saplings under HCH stress. Moreover, a higher presence of lin-degrading genes in the rhizosphere of treated saplings compared to controls confirmed ongoing biodegradation processes. The outcomes help to better understand the processes involved in degradation of persistent pesticides in soil. The mechanism of in-plant isomerization and the identification of metabolites should be the focus of future research.

• Klíčová slova
• Alder tree, HCH, Pesticide, Phytoremediation, Rhizobiome,
• MeSH
• biodegradace * MeSH
• chlorované uhlovodíky metabolismus   MeSH
• cyklopentany metabolismus   MeSH
• hexachlorcyklohexan metabolismus   MeSH
• kořeny rostlin metabolismus   mikrobiologie   MeSH
• kyselina abscisová metabolismus   MeSH
• látky znečišťující půdu * metabolismus   MeSH
• olše * mikrobiologie   MeSH
• oxylipiny metabolismus   MeSH
• pesticidy * metabolismus   MeSH
• půda chemie   MeSH
• půdní mikrobiologie * MeSH
• rhizosféra * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chlorované uhlovodíky MeSH
• cyklopentany MeSH
• hexachlorcyklohexan MeSH
• jasmonic acid MeSH Prohlížeč
• kyselina abscisová MeSH
• látky znečišťující půdu * MeSH
• oxylipiny MeSH
• pesticidy * MeSH
• půda MeSH

Infections caused by antibiotic-drug-resistant microorganisms are a major global health concern, and they result in millions of deaths every year. Methicillin-resistant Staphylococcus aureus (MRSA) is one of such drug-resistant microbial strains, and new and effective antimicrobial agents are desperately needed to combat infections caused by MRSA. In the search for effective anti-MRSA agents, the leaves of Citrus grandis (Rutaceae), also known as C. maxima, were investigated. Implementing a bioassay-guided approach, sinensetin (2), which is a polymethoxyflavone, was isolated as a promising anti-MRSA compound, showing inhibitory activity against three (EMRSA-15, MRSA340802 and MRSA274819; MIC values 128-256 μg/mL) of five MRSA strains tested in the present study. Five other flavonoids 6,7,8,3',4'-pentamethoxyflavone (1), cirsilineol (3), nobiletin (4), 5-desmethylsinensetin (5) and hesperidin (6) were isolated from the dichloromethane extract of this plant. They displayed varied levels of antimicrobial activities against the tested microbial strains, Micrococcus luteus NCTC 7508, Escherichia coli NCTC 12241 and Pseudomonas aeruginosa NCTC 12903, and a fungal strain, Candida albicans ATCC 90028, but not against Staphylococcus aureus NCTC 12981. Sinensetin (2) also exhibited strong antimicrobial activity against the fungal strain C. albicans with an MIC value of 0.0625 mg/mL. The chemical structures of all isolated compounds were unequivocally elucidated by spectroscopic means (1D and 2D NMR and HR-ESIMS). The present study revealed sinensetin (2) as a potential structural template for generating structural analogues and developing anti-MRSA agents and provided scientific evidence supporting the traditional uses of C. grandis in the treatment of microbial infections.

• Klíčová slova
• Citrus grandis, MRSA, Polymethoxyflavonoids, Rutaceae, Sinensetin, Staphylococcus aureus,
• MeSH
• antibakteriální látky farmakologie   izolace a purifikace   chemie   MeSH
• Candida albicans účinky léků   MeSH
• Citrus * chemie   MeSH
• flavonoidy * farmakologie   izolace a purifikace   MeSH
• fytonutrienty farmakologie   izolace a purifikace   MeSH
• listy rostlin * chemie   MeSH
• methicilin rezistentní Staphylococcus aureus * účinky léků   MeSH
• mikrobiální testy citlivosti * MeSH
• molekulární struktura MeSH
• rostlinné extrakty farmakologie   chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• flavonoidy * MeSH
• fytonutrienty MeSH
• rostlinné extrakty MeSH
• sinensetin MeSH Prohlížeč

BACKGROUND: Evidence on the impact of complex neighborhood environment, including air pollution, greenness, and neighborhood socioeconomic deprivation (nSED) on cognitive health in older adults remains scarce. Both cognition and neighborhood environment are associated with physical activity, but little is known about the potential mediating role of physical activity in this association. METHODS: Cross-sectional data of the Czech arm of the HAPIEE cohort study examined 4,178 participants (55.6% women) aged 45-69 years. Global cognitive score was constructed from memory, verbal fluency, and concentration domains. The exposures, assigned to participant's addresses, include 4-year (2000-2003) average concentrations of PM2.5, greenness index calculated from tree crown canopy cover estimation (2000), and census-based nSED characteristics. Physical activity and other covariates were assessed by a questionnaire. Structural equation modelling was used to estimate standardized β coefficients for the relationships between neighborhood environment, physical activity and cognitive performance. RESULTS: After controlling for a range of covariates, global cognitive function was inversely associated with PM2.5 (β = -0.087; 95%CI: 0.122 to -0.052) and nSED (β = -0.147; 95%CI: 0.182 to -0.115), and positively associated with greenness (β = 0.036; 95%CI: 0.001 to 0.069). We identified a weak but statistically significant mediating role of physical activity in the associations of PM2.5 exposures and nSED on global cognitive score. Total mediation proportions ranged from 3.9% to 6.5% for nSED and PM2.5, respectively. CONCLUSIONS: The neighborhood environment was associated with cognitive health in older individuals; the associations were partially mediated by physical activity.

• Klíčová slova
• Ambient air pollution, Cognitive function, Greenness, Physical activity, Socioeconomic deprivation,
• MeSH
• charakteristiky bydlení * MeSH
• charakteristiky okolí bydliště MeSH
• cvičení * MeSH
• kognice * MeSH
• látky znečišťující vzduch analýza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• pevné částice analýza   MeSH
• průřezové studie MeSH
• senioři MeSH
• znečištění ovzduší analýza   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• látky znečišťující vzduch MeSH
• pevné částice MeSH

Recent studies have shown that stem fluxes, although highly variable among trees, can alter the strength of the methane (CH4) sink or nitrous oxide (N2O) source in some forests, but the patterns and magnitudes of these fluxes remain unclear. This study investigated the drivers of subdaily and seasonal variations in stem and soil CH4, N2O and carbon dioxide (CO2) fluxes. CH4, N2O and CO2 fluxes were measured continuously for 19 months in individual stems of two tree species, Eperua falcata (Aubl.) and Lecythis poiteaui (O. Berg), and surrounding soils using an automated chamber system in an upland tropical forest. Subdaily variations in these fluxes were related to environmental and stem physiological (sap flow and stem diameter variations) measurements under contrasting soil water conditions. The results showed that physiological and climatic drivers only partially explained the subdaily flux variations. Stem CH4 and CO2 emissions and N2O uptake varied with soil water content, time of day and between individuals. Stem fluxes decoupled from soil fluxes. Our study contributes to understanding the regulation of stem greenhouse gas fluxes. It suggests that additional variables (e.g. internal gas concentrations, wood-colonising microorganisms, wood density and anatomy) may account for the remaining unexplained variability in stem fluxes, highlighting the need for further studies.

• Klíčová slova
• carbon dioxide, flux, methane, nitrous oxide, soil, subdaily variations, tree stem, upland tropical forest,
• MeSH
• methan * metabolismus   MeSH
• oxid dusný * metabolismus   analýza   MeSH
• oxid uhličitý * metabolismus   MeSH
• půda * chemie   MeSH
• roční období MeSH
• stonky rostlin * metabolismus   MeSH
• stromy * fyziologie   metabolismus   MeSH
• tropické klima * MeSH
• voda metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• methan * MeSH
• oxid dusný * MeSH
• oxid uhličitý * MeSH
• půda * MeSH
• voda MeSH

Uranium mining waste represents a considerable potential source of radon emissions in the nearby environment. The project's aim (2021-2024) was to observe and evaluate the effects of seasonal fluctuations in radon release from former uranium mine dumps No.15 and No.4 in the Příbram area and their influence on the surrounding atmosphere. The objective was to describe the radiation situation and homogeneity of the dumps and to record and analyze the impact of climatic conditions, which contribute to radon transport to the adjacent communities. The progressive phase involved aerial and field measurements of the Ambient Dose Equivalent Rate, detecting hot spots using an unmanned aerial vehicle (UAV), collecting air samples with Lukas cells, and continuously monitoring Radon Activity Concentration. Meteorological data filtered for conditions of temperatures, absence of wind, and no precipitation during night-time hours (from 8:00 p.m. to 6:00 a.m.) identified 890 days between 2005 and 2023. This corresponds to an average of 47 days per year during which conditions conducive to radon transport to the vicinity of the dumps were present, which, as determined, influences radon activity concentrations and their subsequent dispersion into the surrounding environment. These measurements confirmed elevated radon activity concentrations in the ambient air, exceeding several kBq/m³ with measured maxima of 27 kBq/m³. This project's integration of monitoring methods has resulted in a novel, comprehensive approach to determining radon transport from the dump to nearby communities. The ability to accurately identify areas with elevated Radon Activity Concentrations (RAC) plays a crucial role in optimizing strategies to mitigate the impact of radon decay products on public health.

• Klíčová slova
• Aerial measurements, Radon, Spatial analysis, UAV, Uranium mining,
• MeSH
• atmosféra * chemie   MeSH
• hornictví * MeSH
• monitorování radiace * metody   MeSH
• radioaktivní látky znečišťující vzduch * analýza   MeSH
• radon * analýza   MeSH
• roční období * MeSH
• uran * analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• radioaktivní látky znečišťující vzduch * MeSH
• radon * MeSH
• uran * MeSH

The recent surge in popularity of cannabidiol-infused products extends beyond food and supplements to the cosmetic industry. Accurate labeling remains a significant concern, as many products fail to meet advertised cannabidiol content and/or contain psychoactive tetrahydrocannabinol above the permissible levels. In this work, we present the use of an HPLC-UV-MS/MS method for the quantification of five major cannabinoids (cannabidiol, cannabidiolic acid, tetrahydrocannabinol, tetrahydrocannabinolic acid, and cannabigerol) in oil-in-water cosmetic emulsions. The sample extraction is based on dispersing the sample in isopropanol, followed by solid-phase extraction using Bond Elut Plexa PAX cartridge. The viability of this method is demonstrated by comparing matrix effects in samples subjected to solid-phase extraction (0‒11% suppression of cannabidiol ionization) to samples extracted without additional purification steps (0‒84% ionization suppression of cannabidiol ionization). The limit of quantification is determined at 0.1 mg/g and the calibration is employed in the range of 0.1‒50 mg/g for cannabidiol and 0.1‒10 mg/g for all other analytes. Finally, 36 real cosmetic samples from the Czech market were analyzed. Of these, 14 indicated the amount of CBD on the label but only five (36%) were accurate, i.e., within 90‒110% of the labeled amount, and a considerable amount of THC (0.16 ± 0.01 mg/g) was found in one sample. These findings contribute to the cumulating evidence that cannabidiol-based consumer products are often mislabeled and highlight the importance of better regulatory compliance.

• Klíčová slova
• Cannabidiol, Cosmetic creams, Liquid chromatography-mass spectrometry, Oil-in-water emulsions, Tetrahydrocannabinol,
• MeSH
• emulze * chemie   MeSH
• extrakce na pevné fázi * metody   MeSH
• kanabidiol * analýza   MeSH
• kosmetické přípravky * chemie   analýza   MeSH
• limita detekce MeSH
• tandemová hmotnostní spektrometrie * metody   MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• emulze * MeSH
• kanabidiol * MeSH
• kosmetické přípravky * MeSH

Understanding mechanisms driving tropical tree growth is essential for comprehending carbon sequestration and predicting the future of tropical forests amid rapid deforestation. We conducted a natural experiment in Mount Cameroon to identify climatic factors limiting diurnal and seasonal growth in dominant tree species across a 2200-m elevation gradient, from lowland rainforests to montane mist forests with distinct wet and dry seasons. Using high-precision automatic dendrometers, we recorded radial growth rates of 28 tropical tree species from 2015 to 2018, correlating them with rainfall (11 100-2500 mm) and temperatures (23-14°C) across elevations. Significant growth limitations were suggested at both extremes of water availability. Tree growth peaked during the dry and prewet seasons at humid lower elevations and during wet seasons at drier higher elevations. Growth rates increased with soil moisture at higher elevations and peaked at medium soil moisture at lower elevations. Trees grew fastest at lower temperatures relative to their elevation-specific means, with growth limited by high daytime temperatures and promoted by nighttime temperatures. Our results revealed significant interspecific diurnal and seasonal growth variations hindered by both water scarcity and excess in West African rainforests, essential for forecasting and modelling carbon sinks.

• Klíčová slova
• dendrometer, forest, growth dynamics, limitation, soil moisture, temperature, tropics,
• MeSH
• biodiverzita MeSH
• déšť MeSH
• deštný prales MeSH
• nadmořská výška * MeSH
• půda chemie   MeSH
• roční období * MeSH
• stonky rostlin růst a vývoj   fyziologie   MeSH
• stromy * růst a vývoj   fyziologie   MeSH
• teplota MeSH
• tropické klima MeSH
• voda metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Kamerun MeSH
• Názvy látek
• půda MeSH
• voda MeSH

BACKGROUND: COVID-19 vaccine hesitancy (CVH) has become a critical public health issue, with attitudes toward vaccines emerging as a broader social issue. Public debates surrounding vaccines have expanded beyond health considerations to include issues of trust, misinformation, and societal values, making CVH a complex challenge that requires multifaceted solutions. Analyzing the various determinants of CVH is crucial for developing targeted strategies to improve vaccine acceptance in specific countries and to better prepare for future public health crises. However, no study to date has evaluated the determinants of CVH in a representative sample of the Czech population. METHODS: A multiple hierarchical logistic regression was used to analyze the associations between various sociodemographic, trust and attitudinal factors with COVID-19 vaccine acceptance (CVA). The analysis utilized survey data from a representative longitudinal sample of the Czech population (N = 1,407). RESULTS: After controlling for all other factors, trust in official statements from the Ministry of Health was the strongest predictor of CVA, followed by prior positive attitudes toward COVID-19 vaccination (prior to vaccine availability) and older age. Lower trust in COVID-19 misinformation also predicted CVA, while lower interest in COVID-19 media content was associated with CVA. Higher income initially predicted CVA but lost statistical significance after controlling for other variables. Interestingly, education did not play a role in CVA. CONCLUSION: CVH was primarily driven by distrust in government-provided information. Notably, vaccine refusers demonstrated a higher motivation to seek information on the topic, offering a promising opportunity for health policy interventions. Our findings suggest that strategies to reduce CVH should prioritize building trust in state institutions and effectively combating misinformation.

• Klíčová slova
• COVID-19, Misinformation, Trust in institutions, Vaccine acceptance, Vaccine hesitancy,
• MeSH
• COVID-19 * prevence a kontrola   MeSH
• dospělí MeSH
• důvěra * MeSH
• lidé středního věku MeSH
• lidé MeSH
• longitudinální studie MeSH
• mladiství MeSH
• mladý dospělý MeSH
• odkládání očkování * psychologie   statistika a číselné údaje   MeSH
• pacientův souhlas se zdravotní péčí statistika a číselné údaje   psychologie   MeSH
• průzkumy a dotazníky MeSH
• senioři MeSH
• vakcíny proti COVID-19 * aplikace a dávkování   MeSH
• zdraví - znalosti, postoje, praxe MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• vakcíny proti COVID-19 * MeSH

Scenario analysis plays a central role in estimating how global changes affect the relationships linking ecosystem conditions and functioning to human needs. This is particularly true for agroecosystems, which are pivotal to ensure sustainable land planning, ecological management and food security strategies. Soils are key providers of multiple ecosystem services (ES) in agroecosystems but they are very sensitive to global drivers such as changes in climate, land use and cover. How agroecosystems should achieve sustainability, through optimizing soil capacity to supply ES while limiting the occurrence of threats, is a priority of EU policy agendas. Nevertheless, there is currently a lack of a comprehensive framework of scenario-based approaches to assess changes in soil ES (SES) and soil threats (ST). As a part of the project SERENA funded by the European Joint Program on Agricultural Soil Management, this study aims to: i) understand how drivers of global change are commonly studied in the scientific literature; ii) identify how some SES and ST are assessed in scenario-based approaches; iii) provide a preliminary discussion on how soil properties are represented in these approaches. Through a systematic review of 230 published articles related to seven SES and ten ST, this study highlights that not all SES and ST are considered with the same frequency and geographic distribution in scenario-based approaches. Despite a great methodological variability in the assessment and mapping of SES and ST, dominant methodological trends can be identified. SES are mapped more frequently than ST and, specific SES appear more disposed to spatially explicit assessments than others. Due to its novelty and complexity, research on this topic is limited to a small subset of ST or SES and projections of the combined impacts of climate, land use and management changes on multiple ST and SES should be a scientific priority to help policy makers.

• Klíčová slova
• Agroecosystems, Indicators, Scenario-based, Soil ecosystem services, Soil threats,
• MeSH
• ekosystém * MeSH
• klimatické změny MeSH
• monitorování životního prostředí metody   MeSH
• půda * MeSH
• zachování přírodních zdrojů * metody   MeSH
• zemědělství * metody   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• systematický přehled MeSH
• Názvy látek
• půda * MeSH