BACKGROUND: CrossFitⓇ sessions and competitions are characterized by high-intensity challenges that combine aerobic and anaerobic activities with short recovery periods. As a result, effective nutritional practices play a crucial role in optimizing performance and enhancing recovery. Therefore, nutritional practices are central to optimizing performance and accelerating recovery. This review aims to summarize current evidence on nutritional and ergogenic aid responses to CrossFitⓇ practice. METHODS: The search was conducted in four electronic databases (PubMed, Web of Science, Scopus and SportDiscus). Gray literature was also extracted for studies exploring the nutritional habits of CrossFitⓇ participants as well as the ingestion of ergogenic aids. In addition, a meta-analysis was conducted to examine the impacts of dietary habits and ergogenic aids on performance. RESULTS: Forty-nine studies met the eligibility criteria and were included in the current review. Carbohydrate intake was below the recommendations for athletes, although protein ingestion remains adequate. High-carbohydrate diets had a positive effect on CrossFitⓇ performance. The evidence concerning the effects of a ketogenic diet on performance is limited. When used prior to or during the workout, the impact of carbohydrates on CrossFitⓇ performance was negligible, whereas the effect of caffeine was significant. Ergogenic aids, particularly creatine and protein, are commonly used by CrossFitⓇ participants. CONCLUSION: The standard diets recommended to CrossFitⓇ participants need to be revised because they are characterized by lower values of carbohydrates. Caffeine should be used prior to or during the CrossFitⓇ sessions. Regarding the impact of ergogenic aids on recovery, future studies are needed.

• Klíčová slova
• Carbohydrates, caffeine, conditioning, energy, performance, strength,
• MeSH
• dieta MeSH
• dietní proteiny aplikace a dávkování   MeSH
• dietní sacharidy aplikace a dávkování   MeSH
• fyziologie sportovní výživy * MeSH
• ketogenní dieta MeSH
• kofein aplikace a dávkování   MeSH
• kondiční příprava * MeSH
• kreatin aplikace a dávkování   MeSH
• látky zvyšující výkon aplikace a dávkování   MeSH
• lidé MeSH
• sportovní výkon * fyziologie   MeSH
• stravovací zvyklosti MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• scoping review MeSH
• Názvy látek
• dietní proteiny MeSH
• dietní sacharidy MeSH
• kofein MeSH
• kreatin MeSH
• látky zvyšující výkon MeSH

BACKGROUND: The current negative trend in the physical behavior and lifestyle of the population therefore requires adequate changes in the professional training of physiotherapists. OBJECTIVES: This study aimed to determine the structure and differences in the weekly physical activity (PA) of Czech physiotherapy students, the use of wearables in physiotherapy professional training, and the attitude of physiotherapy students toward PA and the use of wearables in physiotherapy practice. METHODS: Between 2013 and 2022, 412 physiotherapy students participated in a PA-monitoring study using questonnaires International Physical Activity Questionnaire-long form, Motives for Physical Activity Measure-Revise, pedometers, Garmin Vívofit and Axivity AX3 accelerometers. RESULTS: A retrospective analysis of physiotherapy students PA drew attention to insufficient weekly PA and insufficient achievement of the PA recommendation of at least 60 min five times a week (55% of men and 41% of women). Instrumental PA monitoring allowed analyzing individual daily PA and structure of weekly PA. Highest PA indicated men (14,102 steps/day) and women (12,724 steps/day) of the 1st study year on Tuesday. The lowest PA (9,488 steps/day for men and 8,815 steps/day for women), were observed in the 4th study year on Sundays. The recommended target of 11,000 steps per day was achieved by 40% of the men and 46% of the women. Wearables enhanced participants PA motivation (51%). CONCLUSIONS: The inclusion of weekly PA monitoring in the professional training of physiotherapists ensured a deeper insight into the possibilities of PA monitoring in physiotherapy practice . Students are prepared to use wearables more widely to improve physical therapy practice.

The study demonstrated the need for greater support for physical activity (PA) of physiotherapy students during their studies.Incorporating comprehensive weekly PA monitoring into physiotherapy education is essential, especially with the rapid development of wearable technology.Monitoring PA using different methods enabled students to better assess the possibilities of using wearables in physiotherapy practice.

• Klíčová slova
• Physical activity, lifestyle, monitoring, professional education, wearables,
• MeSH
• cvičení * fyziologie   MeSH
• dospělí MeSH
• fyzioterapeuti * výchova   MeSH
• lidé MeSH
• mladý dospělý MeSH
• motivace MeSH
• postoj zdravotnického personálu MeSH
• průzkumy a dotazníky MeSH
• retrospektivní studie MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

BACKGROUND AND OBJECTIVE: Digital Holographic Microscopy provides a new kind of quantitative image data about live cells' in vitro activities. Apart from non-invasive and staining-free imaging, it offers topological weighting of cell mass. This led us to develop a particular tool for assessing cell mass dynamics. METHODS: Programming language Python and a training set of time-lapse images of adherent HT-1080 cells derived from human fibrosarcoma taken with dry objective 40x/0.95 at 30-second intervals were used to create the Analytical Image Differencing (AID) method. RESULTS: The AID makes the best of these new data by evaluating the difference between the chosen two quantitative phase images from the time-lapse series. The contribution of the method is demonstrated on hiQPI (Holographic Incoherent-light-source Quantitative Phase Imaging) image data taken with a Q-phase microscope. The analysis outputs are graphical and complemented with numerical data. To underscore the significance of the Analytical Image Differencing (AID) method, an initial pilot experiment was conducted to show the available analyses of sequential overlapping images capturing the movement of cancer cells. Notably, besides defining changes in areas used by the cell (newly or steadily occupied or better abandoned) it is an introduction to the zero-line concept, which denotes spots of tranquility among continuously moving surroundings. CONCLUSIONS: The measurement of zero-line length has emerged as a novel biomarker for characterizing cell mass transfer. The sensitivity of phase change measurements is demonstrated. The noise quality of input images obtained with incoherent (hiQPI) and coherent (QPI) methods is compared. The resulting effect on the AID method output is also shown. The findings of this study introduce a novel approach to evaluating cellular behavior in vitro. The concept emerged as a particularly noteworthy outcome. Collectively, these results highlight the substantial potential of AID in advancing the field of cancer cells biology, particularly.

• Klíčová slova
• Biophysics, Cancer cell migration, Digital holographic microscopy, Image processing, Live cell imaging, Non-invasive, Quantitative phase imaging, Staining-free imaging,
• MeSH
• algoritmy MeSH
• časosběrné zobrazování MeSH
• fibrosarkom patologie   MeSH
• holografie metody   MeSH
• lidé MeSH
• mikroskopie MeSH
• nádorové buněčné linie MeSH
• počítačové zpracování obrazu MeSH
• pohyb buněk * MeSH
• programovací jazyk MeSH
• software MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

BACKGROUND: The health benefits of regular exercise and physical activity in people with multiple sclerosis (MS) have been described in numerous studies. Despite this, research from global and North American populations suggests that people with MS are less physically active. However, not enough data are available from Central European countries. To address this gap we conducted a study to evaluate the physical activity of people with MS and to compare it with a group of healthy controls. METHODS: Physical activity levels were assessed using the Godin Leisure-Time Exercise Questionnaire (GLTEQ) for both the MS and healthy control groups. The Pearson χ2 test was employed to analyse the effect of age, gender and BMI on physical activity-category (GLTEQ) within the MS and HC groups. RESULTS: The study included 729 participants, comprising 354 individuals with MS and 375 healthy controls. Participants with MS exhibited substantially lower levels of physical activity than the healthy controls (p < 0.001) and engaged in exercise less frequently and regularly (p < 0.001). Lack of time was the primary barrier to participation in physical activity for both groups. However, physical limitations were a common obstacle for individuals with MS, whereas healthy controls more often cited the absence of equipment or other reasons. Women with MS were significantly more likely to abstain from physical activity, while men with MS were more likely to exercise regularly. Unlike healthy controls, the level of physical activity in individuals with MS was not influenced by BMI. CONCLUSION: Compared to healthy individuals, people with MS have reduced levels of physical activity and are less likely to exercise regularly. They also experience various barriers and motivational factors affecting their physical activity.

• Klíčová slova
• Godin leisure-time exercise questionnaire, Multiple sclerosis, Physical activity,
• MeSH
• cvičení * MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• průzkumy a dotazníky MeSH
• roztroušená skleróza * patofyziologie   epidemiologie   psychologie   MeSH
• sexuální faktory MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Evropa MeSH

The cognitive processing of learning materials has been extensively studied within various cognitive theories. Self-regulated learning (SRL) is also recognized as a key factor in learning efficiency. However, evidence linking SRL to learning outcomes remains inconclusive, particularly regarding objective behavioral data during learning. This study presents an original empirical dataset on eye-tracking activity during learning, examining the effects of metacognitive prompts and multimedia content on cognitive processing and learning outcomes. A controlled laboratory experiment with a 2 × 2 mixed factorial design involved 110 university students, resulting in 84 complete recordings of eye-movement activity during learning. Participants studied scientific materials in text-only and multimedia formats, with one group receiving metacognitive prompts and the control group receiving general instructions. Learning performance was assessed via a post-test, and eye-tracking technology captured gaze patterns to provide insights into cognitive engagement and attention distribution. Applications extend to e-learning, virtual environments, and user interface design. While the dataset has some methodological limitations, it remains a robust resource for studying cognitive processes and optimizing educational technologies.

• MeSH
• kognice MeSH
• lidé MeSH
• mladý dospělý MeSH
• pohyby očí * MeSH
• sebekontrola * MeSH
• technologie sledování pohybu očí * MeSH
• učení * MeSH
• Check Tag
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• dataset MeSH

The sodium/calcium exchanger (NCX) type 1 has been well described in various cancers, but little is known about the other two NCX types (NCX2 and NCX3). In this study, we used the selective blocker of NCX3 - YM-244769 to investigate changes in apoptosis induction, migration, proliferation, intracellular calcium and ATP in four cancer cell lines - DLD1, HeLa, MDA-MB-231 and JIMT1. In all four cell lines we observed a concentration-dependent increase in the number of apoptotic cells, as well as reduced migration and proliferation. Induction of hypoxic conditions did not alter the response of these cells to YM-244769 in any of the above-mentioned parameters. These results indicate the role of NCX3 in cancer cell migration, proliferation and apoptosis, as inhibition of NCX1 by the specific blocker SEA0400 had no significant effect on these parameters. However, we verified the effect of NCX3 inhibition by using CRISPR/Cas9 to generate clones in which the SLC8A3 (NCX3) gene was deleted, and we obtained the same results. In addition, mitochondrial respiration was impaired in the clones with NCX3 knocked-out, suggesting that NCX3 also play a role in bioenergetics. In conclusion, we have clearly shown that NCX3 plays an important anti-apoptotic, pro-migratory and proliferative role in the cancer cells by affecting mitochondrial bioenergetics, thus supporting their survival and fate.

• Klíčová slova
• Breast cancer cell lines, Cervical cancer cell line, Colorectal cancer cell line, Migration, Mitochondrial respiration, Proliferation, Sodium/calcium exchanger type 3,
• MeSH
• apoptóza účinky léků   MeSH
• lidé MeSH
• mitochondrie metabolismus   účinky léků   MeSH
• nádorové buněčné linie MeSH
• nádory * metabolismus   patologie   genetika   MeSH
• pohyb buněk účinky léků   MeSH
• proliferace buněk účinky léků   MeSH
• pumpa pro výměnu sodíku a vápníku * metabolismus   genetika   antagonisté a inhibitory   MeSH
• vápník metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• pumpa pro výměnu sodíku a vápníku * MeSH
• SLC38A3 protein, human MeSH Prohlížeč
• vápník MeSH

BACKGROUND: The use of statistical parameter mapping (SPM) to compare gait kinematics of children at different ages seems to be a more appropriate tool to describe the differences than simply describing the maxima and minima on the curves. RESEARCH QUESTION: Does lower limb kinematic waveforms differ during gait in normally developing preschool children? METHODS: In a cross-sectional study, SPM was used to compare kinematic waveforms of typically developing preschool children at ages 2, 3, and 6 years (n = 42). RESULTS: Differences in internal rotation foot angle between 2-year-olds and 3-, 6-year-olds in 22-55 % lower in 2-year-olds but 85-100 % greater in 2-year-olds. Greater internal rotation of the knee in 2-year-olds versus 6-year-olds in 13-25 % of the stance phase. Lower knee abduction in 2-year-olds versus 6-year-olds in the first 13 % of the stance phase. SIGNIFICANCE: Comparison of the waveforms of the angle may provide a clearer understanding of the differences in gait kinematics in children at different ages.

• Klíčová slova
• Children, Develop, Gait, Kinematics, Statistical parameter mapping,
• MeSH
• biomechanika MeSH
• chůze (způsob) * fyziologie   MeSH
• dítě MeSH
• kolenní kloub * fyziologie   MeSH
• lidé MeSH
• předškolní dítě MeSH
• průřezové studie MeSH
• rozsah kloubních pohybů * fyziologie   MeSH
• věkové faktory MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Promoting healthy aging through physical activity (PA) is crucial as the global population grows older. Traditional interventions often fail to engage older adults, underlining the need for personalized, timely approaches. Smartphone-delivered PA interventions can offer personalized support during opportune moments for behavioral change. The current study examined whether the receptivity of inactive older adults influences compliance with mHealth walking suggestions after inactivity, and explored their experiences with it. Thirty healthy older adults (mean age 73.9 years) participated in the study and answered event-based EMA questionnaires via HealthReact after each 30-minute inactivity period. Emotions, physical complaints, intention, self-efficacy, perceived walking, and environmental permissiveness were assessed. Walking suggestions followed each EMA, and semi-structured interviews were conducted post-study. Multilevel logistic regressions in R were applied, and qualitative data were thematically analyzed using NVivo. Results show that higher intention, self-efficacy, and environmental permissiveness positively correlated with compliance, while higher perceived walking negatively correlated. Participants generally found the suggestions motivating and well-timed, but some reported increased alertness and pressure. Consequently, tailoring interventions to individual needs and targeting receptive moments can enhance compliance and promote healthier aging through increased PA. Future mobile interventions should consider self-efficacy, intention, prior activity, and environmental conditions to improve effectiveness.

• Klíčová slova
• elderly, event‐based EMA, physical activity, recommendations,
• MeSH
• adherence pacienta * psychologie   MeSH
• chůze * psychologie   MeSH
• chytrý telefon MeSH
• cvičení psychologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• longitudinální studie MeSH
• podpora zdraví * metody   MeSH
• sebeuplatnění MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• telemedicína MeSH
• úmysl MeSH
• zdravé stárnutí * psychologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Interferon‑induced transmembrane proteins (IFITMs) are frequently overexpressed in cancer cells, including cervical carcinoma cells, and play a role in the progression of various cancer types. However, their mechanisms of action remain incompletely understood. In the present study, by employing a combination of surface membrane protein isolation and quantitative mass spectrometry, it was comprehensively described how the IFITM1 protein influences the composition of the cervical cancer cell surfaceome. Additionally, the effects of interferon‑γ on protein expression and cell surface exposure were evaluated in the presence and absence of IFITM1. The IFITM1‑regulated membrane and membrane‑associated proteins identified are involved mainly in processes such as endocytosis and lysosomal transport, cell‑cell and cell‑extracellular matrix adhesion, antigen presentation and the immune response. To complement the proteomic data, gene expression was analyzed using reverse transcription‑quantitative PCR to distinguish whether the observed changes in protein levels were attributable to transcriptional regulation or differential protein dynamics. Furthermore, the proteomic and gene expression data are supported by functional studies demonstrating the impact of the IFITM1 and IFITM3 proteins on the adhesive, migratory and invasive capabilities of cervical cancer cells, as well as their interactions with immune cells.

• Klíčová slova
• antigen presentation, cervical cancer, immunosurveillance, interferon‑induced transmembrane proteins, invasion, mass spectrometry, membrane protein, migration, surfaceome,
• MeSH
• buněčná adheze MeSH
• diferenciační antigeny * metabolismus   genetika   MeSH
• fenotyp MeSH
• interferon gama farmakologie   metabolismus   MeSH
• lidé MeSH
• membránové proteiny * metabolismus   genetika   MeSH
• nádorové buněčné linie MeSH
• nádory děložního čípku * patologie   genetika   metabolismus   imunologie   MeSH
• pohyb buněk MeSH
• proteiny vázající RNA * metabolismus   genetika   MeSH
• proteom * MeSH
• proteomika metody   MeSH
• regulace genové exprese u nádorů MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• diferenciační antigeny * MeSH
• IFITM3 protein, human MeSH Prohlížeč
• interferon gama MeSH
• leu-13 antigen MeSH Prohlížeč
• membránové proteiny * MeSH
• proteiny vázající RNA * MeSH
• proteom * MeSH

Intracranial human brain recordings from multiple implanted depth electrodes using stereo-EEG (sEEG) technology for seizure localization provide unique local field potential signals (LFP) sampled with standard macro- and special micro-electrode contacts. Over one hundred macro- and micro-contact LFP signals localized in particular brain regions were recorded from each sEEG monitoring case as patients engaged in an automated battery of verbal memory and non-verbal gaze movement tasks. Subject eye and vocal responses in both visual and auditory task versions were automatically detected in Polish, Czech, and Slovak languages with accurate timing of the correct and incorrect verbal responses using our web-based transcription tool. The behavioral events, LFP and pupillometric signals were synchronized and stored in a standard BIDS data structure with corresponding metadata. Each dataset contains recordings from at least one battery task performed over at least one day. The same set of 180 common nouns in the three languages was used across different battery tasks and recording days to enable the analysis of selective responses to specific word stimuli.

• MeSH
• elektroencefalografie MeSH
• jazyk (prostředek komunikace) MeSH
• kognice * MeSH
• lidé MeSH
• mozek * fyziologie   MeSH
• pohyby očí MeSH
• technologie sledování pohybu očí MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• dataset MeSH